产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF7407, RRID:AB_2843847.
展开/折叠
ERK-3; ERK3; Extracellular signal regulated kinase 3; Extracellular signal regulated kinase p97; Extracellular signal-regulated kinase 3; HsT17250; MAP kinase 6; MAP kinase isoform p97; MAPK 6; MAPK6; Mitogen activated protein kinase 6; Mitogen-activated protein kinase 6; MK06_HUMAN; p97 MAPK; p97-MAPK; PRKM6; Protein kinase mitogen activated 5; Protein kinase mitogen activated 6;
抗原和靶标
Highest expression in the skeletal muscle, followed by the brain. Also found in heart, placenta, lung, liver, pancreas, kidney and skin fibroblasts.
- Q16659 MK06_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAEKFESLMNIHGFDLGSRYMDLKPLGCGGNGLVFSAVDNDCDKRVAIKKIVLTDPQSVKHALREIKIIRRLDHDNIVKVFEILGPSGSQLTDDVGSLTELNSVYIVQEYMETDLANVLEQGPLLEEHARLFMYQLLRGLKYIHSANVLHRDLKPANLFINTEDLVLKIGDFGLARIMDPHYSHKGHLSEGLVTKWYRSPRLLLSPNNYTKAIDMWAAGCIFAEMLTGKTLFAGAHELEQMQLILESIPVVHEEDRQELLSVIPVYIRNDMTEPHKPLTQLLPGISREALDFLEQILTFSPMDRLTAEEALSHPYMSIYSFPMDEPISSHPFHIEDEVDDILLMDETHSHIYNWERYHDCQFSEHDWPVHNNFDIDEVQLDPRALSDVTDEEEVQVDPRKYLDGDREKYLEDPAFDTNYSTEPCWQYSDHHENKYCDLECSHTCNYKTRSSSYLDNLVWRESEVNHYYEPKLIIDLSNWKEQSKEKSDKKGKSKCERNGLVKAQIALEEASQQLAGKEREKNQGFDFDSFIAGTIQLSSQHEPTDVVDKLNDLNSSVSQLELKSLISKSVSQEKQEKGMANLAQLEALYQSSWDSQFVSGGEDCFFINQFCEVRKDEQVEKENTYTSYLDKFFSRKEDTEMLETEPVEDGKLGERGHEEGFLNNSGEFLFNKQLESIGIPQFHSPVGSPLKSIQATLTPSAMKSSPQIPHQTYSSILKHLN
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q16659 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K4 | Ubiquitination | Uniprot | |
| T54 | Phosphorylation | Uniprot | |
| K60 | Ubiquitination | Uniprot | |
| K141 | Ubiquitination | Uniprot | |
| S145 | Phosphorylation | Uniprot | |
| K154 | Ubiquitination | Uniprot | |
| Y182 | Phosphorylation | Uniprot | |
| S189 | Phosphorylation | Q16659 (MAPK6) , Q13177 (PAK2) , O75914 (PAK3) | Uniprot |
| K195 | Ubiquitination | Uniprot | |
| Y209 | Phosphorylation | Uniprot | |
| K276 | Ubiquitination | Uniprot | |
| Y357 | Phosphorylation | Uniprot | |
| S363 | Phosphorylation | Uniprot | |
| S386 | Phosphorylation | Uniprot | |
| T389 | Phosphorylation | Uniprot | |
| K408 | Ubiquitination | Uniprot | |
| K447 | Ubiquitination | Uniprot | |
| S450 | Phosphorylation | Uniprot | |
| S452 | Phosphorylation | Uniprot | |
| Y453 | Phosphorylation | Uniprot | |
| Y467 | Phosphorylation | Uniprot | |
| Y468 | Phosphorylation | Uniprot | |
| K471 | Ubiquitination | Uniprot | |
| K480 | Ubiquitination | Uniprot | |
| S483 | Phosphorylation | Uniprot | |
| K502 | Ubiquitination | Uniprot | |
| S511 | Phosphorylation | Uniprot | |
| K517 | Ubiquitination | Uniprot | |
| S556 | Phosphorylation | Uniprot | |
| S558 | Phosphorylation | Uniprot | |
| K563 | Ubiquitination | Uniprot | |
| K568 | Ubiquitination | Uniprot | |
| K621 | Ubiquitination | Uniprot | |
| K631 | Ubiquitination | Uniprot | |
| K636 | Ubiquitination | Uniprot | |
| T644 | Phosphorylation | Uniprot | |
| K651 | Ubiquitination | Uniprot | |
| S665 | Phosphorylation | Uniprot | |
| K672 | Ubiquitination | Uniprot | |
| S684 | Phosphorylation | P06493 (CDK1) | Uniprot |
| S688 | Phosphorylation | P06493 (CDK1) | Uniprot |
| K691 | Ubiquitination | Uniprot | |
| T698 | Phosphorylation | P06493 (CDK1) | Uniprot |
| K703 | Ubiquitination | Uniprot | |
| S704 | Phosphorylation | Uniprot | |
| S705 | Phosphorylation | P06493 (CDK1) | Uniprot |
翻译修饰 - Q16659 作为激酶
研究背景
Atypical MAPK protein. Phosphorylates microtubule-associated protein 2 (MAP2) and MAPKAPK5. The precise role of the complex formed with MAPKAPK5 is still unclear, but the complex follows a complex set of phosphorylation events: upon interaction with atypical MAPKAPK5, ERK3/MAPK6 is phosphorylated at Ser-189 and then mediates phosphorylation and activation of MAPKAPK5, which in turn phosphorylates ERK3/MAPK6. May promote entry in the cell cycle (By similarity).
Phosphorylated at Ser-189 by PAK1, PAK2 and PAK3 resulting in catalytic activation. Phosphorylated by MAPKAPK5 at other sites.
Ubiquitination at Met-1 leads to degradation by the proteasome pathway.
Cytoplasm. Nucleus.
Note: Translocates to the cytoplasm following interaction with MAPKAPK5.
Highest expression in the skeletal muscle, followed by the brain. Also found in heart, placenta, lung, liver, pancreas, kidney and skin fibroblasts.
Heterodimer with ERK4/MAPK4. Interacts with (via FRIEDE motif) MAPKAPK5 (By similarity). Interacts with UBE3A; this interaction may be indirect and mediated by HERC2, possibly via HERC2 interaction with NEURL4.
In contrast to classical MAPKs, the TXY motif within the activation loop is replaced by the SEG motif, whose phosphorylation activates the MAP kinases.
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
研究领域
· Organismal Systems > Immune system > IL-17 signaling pathway. (View pathway)
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