产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF7408, RRID:AB_2843848.
展开/折叠
KIAA1108; TBC 1; TBC; TBC1 (tre 2/USP6, BUB2, cdc16) domain family, member 1; TBC1; TBC1 domain family member 1; Tbc1d1; TBCD1_HUMAN;
抗原和靶标
- Q86TI0 TBCD1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEPITFTARKHLLSNEVSVDFGLQLVGSLPVHSLTTMPMLPWVVAEVRRLSRQSTRKEPVTKQVRLCVSPSGLRCEPEPGRSQQWDPLIYSSIFECKPQRVHKLIHNSHDPSYFACLIKEDAVHRQSICYVFKADDQTKVPEIISSIRQAGKIARQEELHCPSEFDDTFSKKFEVLFCGRVTVAHKKAPPALIDECIEKFNHVSGSRGSESPRPNPPHAAPTGSQEPVRRPMRKSFSQPGLRSLAFRKELQDGGLRSSGFFSSFEESDIENHLISGHNIVQPTDIEENRTMLFTIGQSEVYLISPDTKKIALEKNFKEISFCSQGIRHVDHFGFICRESSGGGGFHFVCYVFQCTNEALVDEIMMTLKQAFTVAAVQQTAKAPAQLCEGCPLQSLHKLCERIEGMNSSKTKLELQKHLTTLTNQEQATIFEEVQKLRPRNEQRENELIISFLRCLYEEKQKEHIHIGEMKQTSQMAAENIGSELPPSATRFRLDMLKNKAKRSLTESLESILSRGNKARGLQEHSISVDLDSSLSSTLSNTSKEPSVCEKEALPISESSFKLLGSSEDLSSDSESHLPEEPAPLSPQQAFRRRANTLSHFPIECQEPPQPARGSPGVSQRKLMRYHSVSTETPHERKDFESKANHLGDSGGTPVKTRRHSWRQQIFLRVATPQKACDSSSRYEDYSELGELPPRSPLEPVCEDGPFGPPPEEKKRTSRELRELWQKAILQQILLLRMEKENQKLQASENDLLNKRLKLDYEEITPCLKEVTTVWEKMLSTPGRSKIKFDMEKMHSAVGQGVPRHHRGEIWKFLAEQFHLKHQFPSKQQPKDVPYKELLKQLTSQQHAILIDLGRTFPTHPYFSAQLGAGQLSLYNILKAYSLLDQEVGYCQGLSFVAGILLLHMSEEEAFKMLKFLMFDMGLRKQYRPDMIILQIQMYQLSRLLHDYHRDLYNHLEEHEIGPSLYAAPWFLTMFASQFPLGFVARVFDMIFLQGTEVIFKVALSLLGSHKPLILQHENLETIVDFIKSTLPNLGLVQMEKTINQVFEMDIAKQLQAYEVEYHVLQEELIDSSPLSDNQRMDKLEKTNSSLRKQNLDLLEQLQVANGRIQSLEATIEKLLSSESKLKQAMLTLELERSALLQTVEELRRRSAEPSDREPECTQPEPTGD
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q86TI0 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S69 | Phosphorylation | Uniprot | |
| Y90 | Phosphorylation | Uniprot | |
| Y113 | Phosphorylation | Uniprot | |
| K199 | Ubiquitination | Uniprot | |
| S211 | Phosphorylation | Uniprot | |
| S235 | Phosphorylation | P31749 (AKT1) | Uniprot |
| S237 | Phosphorylation | Q13131 (PRKAA1) , P54646 (PRKAA2) | Uniprot |
| K248 | Ubiquitination | Uniprot | |
| S263 | Phosphorylation | Uniprot | |
| S408 | Phosphorylation | Uniprot | |
| T410 | Phosphorylation | Uniprot | |
| K497 | Acetylation | Uniprot | |
| K501 | Acetylation | Uniprot | |
| S503 | Phosphorylation | Uniprot | |
| T505 | Phosphorylation | Uniprot | |
| S507 | Phosphorylation | Uniprot | |
| S513 | Phosphorylation | Uniprot | |
| S527 | Phosphorylation | Uniprot | |
| S559 | Phosphorylation | Uniprot | |
| S565 | Phosphorylation | Uniprot | |
| S566 | Phosphorylation | Uniprot | |
| S570 | Phosphorylation | Uniprot | |
| S571 | Phosphorylation | Uniprot | |
| S573 | Phosphorylation | Uniprot | |
| S575 | Phosphorylation | Uniprot | |
| S585 | Phosphorylation | Uniprot | |
| T596 | Phosphorylation | P31749 (AKT1) , Q13131 (PRKAA1) | Uniprot |
| S598 | Phosphorylation | Uniprot | |
| S614 | Phosphorylation | Uniprot | |
| S618 | Phosphorylation | Uniprot | |
| S627 | Phosphorylation | Uniprot | |
| S649 | Phosphorylation | Uniprot | |
| T652 | Phosphorylation | Uniprot | |
| S660 | Phosphorylation | Uniprot | |
| T671 | Phosphorylation | Uniprot | |
| S695 | Phosphorylation | Uniprot | |
| K743 | Ubiquitination | Uniprot | |
| K754 | Ubiquitination | Uniprot | |
| K768 | Ubiquitination | Uniprot | |
| K826 | Ubiquitination | Uniprot | |
| K835 | Ubiquitination | Uniprot | |
| Y880 | Phosphorylation | Uniprot | |
| K1092 | Ubiquitination | Uniprot | |
| S1137 | Phosphorylation | Uniprot |
研究背景
May act as a GTPase-activating protein for Rab family protein(s). May play a role in the cell cycle and differentiation of various tissues. Involved in the trafficking and translocation of GLUT4-containing vesicles and insulin-stimulated glucose uptake into cells (By similarity).
Insulin-stimulated phosphorylation by AKT family kinases stimulates SLC2A4/GLUT4 translocation.
Nucleus.
Interacts with APPL2 (via BAR domain); interaction is dependent of TBC1D1 phosphorylation at Ser-235; interaction diminishes the phosphorylation of TBC1D1 at Thr-596, resulting in inhibition of SLC2A4/GLUT4 translocation and glucose uptake.
研究领域
· Environmental Information Processing > Signal transduction > AMPK signaling pathway. (View pathway)
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