产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF7422, RRID:AB_2843862.
展开/折叠
3''-pyrimidine nucleotidase; 5' nucleotidase, deoxy (pyrimidine); 5' nucleotidase, deoxy (pyrimidine), cytosolic type C; 5''(3'')-deoxyribonucleotidase; 5'(3')-deoxyribonucleotidase, cytosolic type; 5', 3'-nucleotidase, cytosolic; 5-prime,3-prime-nucleotidase, cytosolic; cdN; Cytosolic 5''; cytosolic 5',3'-pyrimidine nucleotidase; cytosolic type; cytosolic type C,5',3'-nucleotidase; cytosolic,uridine 5'-monophosphate phosphohydrolase 2; Deoxy 5' nucleotidase 1; Deoxy-5''-nucleotidase 1; Deoxyribonucleotidase, cytosolic, 1; DNT; dNT-1; dNT1; Nt5c; NT5C_HUMAN; P5N2; PN-I; PN-II; Pyrimidine 5-prime nucleotidase 2; UMPH2; uridine 5'-monophosphate phosphohydrolase 2; uridine 5-prime monophosphate hydrolase 2;
抗原和靶标
- Q8TCD5 NT5C_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MARSVRVLVDMDGVLADFEAGLLRGFRRRFPEEPHVPLEQRRGFLAREQYRALRPDLADKVASVYEAPGFFLDLEPIPGALDAVREMNDLPDTQVFICTSPLLKYHHCVGEKYRWVEQHLGPQFVERIILTRDKTVVLGDLLIDDKDTVRGQEETPSWEHILFTCCHNRHLVLPPTRRRLLSWSDNWREILDSKRGAAQRE
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q8TCD5 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S63 | Phosphorylation | Uniprot | |
| Y65 | Phosphorylation | Uniprot | |
| K112 | Acetylation | Uniprot | |
| K112 | Ubiquitination | Uniprot | |
| K134 | Ubiquitination | Uniprot | |
| K146 | Acetylation | Uniprot | |
| K146 | Ubiquitination | Uniprot | |
| S182 | Phosphorylation | Uniprot | |
| S184 | Phosphorylation | Uniprot | |
| K194 | Ubiquitination | Uniprot |
研究背景
Dephosphorylates the 5' and 2'(3')-phosphates of deoxyribonucleotides, with a preference for dUMP and dTMP, intermediate activity towards dGMP, and low activity towards dCMP and dAMP.
Cytoplasm.
Detected in skeletal muscle, heart and pancreas.
Homodimer.
Belongs to the 5'(3')-deoxyribonucleotidase family.
研究领域
· Metabolism > Nucleotide metabolism > Purine metabolism.
· Metabolism > Nucleotide metabolism > Pyrimidine metabolism.
· Metabolism > Metabolism of cofactors and vitamins > Nicotinate and nicotinamide metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
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