| 产品: | 磷酸化 SGK3 (Ser486) 抗体 |
| 货号: | AF7430 |
| 描述: | Rabbit polyclonal antibody to Phospho-SGK3 (Ser486) |
| 应用: | WB IHC |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 50-60kDa; 57kD(Calculated). |
| 蛋白号: | Q96BR1 |
| RRID: | AB_2843870 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF7430, RRID:AB_2843870.
展开/折叠
CISK; Cytokine independent survival kinase; DKFZp781N0293; Serine/threonine protein kinase Sgk3; Serine/threonine-protein kinase Sgk3; Serum/glucocorticoid regulated kinase 3; Serum/glucocorticoid regulated kinase family member 3; Serum/glucocorticoid regulated kinase like; Serum/glucocorticoid-regulated kinase 3; Serum/glucocorticoid-regulated kinase-like; SGK 2; SGK 3; SGK2; Sgk3; SGK3_HUMAN; SGKL;
抗原和靶标
Expressed in most tissues with highest levels in pancreas, kidney liver, heart and brain and lower levels in lung, placenta and skeletal muscle. Expression is higher in ER-positive breast tumors than ER-negative breast tumors.
- Q96BR1 SGK3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MQRDHTMDYKESCPSVSIPSSDEHREKKKRFTVYKVLVSVGRSEWFVFRRYAEFDKLYNTLKKQFPAMALKIPAKRIFGDNFDPDFIKQRRAGLNEFIQNLVRYPELYNHPDVRAFLQMDSPKHQSDPSEDEDERSSQKLHSTSQNINLGPSGNPHAKPTDFDFLKVIGKGSFGKVLLAKRKLDGKFYAVKVLQKKIVLNRKEQKHIMAERNVLLKNVKHPFLVGLHYSFQTTEKLYFVLDFVNGGELFFHLQRERSFPEHRARFYAAEIASALGYLHSIKIVYRDLKPENILLDSVGHVVLTDFGLCKEGIAISDTTTTFCGTPEYLAPEVIRKQPYDNTVDWWCLGAVLYEMLYGLPPFYCRDVAEMYDNILHKPLSLRPGVSLTAWSILEELLEKDRQNRLGAKEDFLEIQNHPFFESLSWADLVQKKIPPPFNPNVAGPDDIRNFDTAFTEETVPYSVCVSSDYSIVNASVLEADDAFVGFSYAPPSEDLFL
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q96BR1 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T32 | Phosphorylation | Uniprot | |
| K75 | Methylation | Uniprot | |
| S121 | Phosphorylation | Uniprot | |
| S126 | Phosphorylation | Uniprot | |
| S129 | Phosphorylation | Uniprot | |
| K139 | Ubiquitination | Uniprot | |
| K158 | Ubiquitination | Uniprot | |
| K166 | Ubiquitination | Uniprot | |
| Y188 | Phosphorylation | Uniprot | |
| K191 | Ubiquitination | Uniprot | |
| K216 | Ubiquitination | Uniprot | |
| S315 | Phosphorylation | Uniprot | |
| T318 | Phosphorylation | Uniprot | |
| T320 | Phosphorylation | O15530 (PDPK1) | Uniprot |
| T324 | Phosphorylation | Uniprot | |
| K407 | Ubiquitination | Uniprot | |
| S486 | Phosphorylation | Uniprot |
翻译修饰 - Q96BR1 作为激酶
研究背景
Serine/threonine-protein kinase which is involved in the regulation of a wide variety of ion channels, membrane transporters, cell growth, proliferation, survival and migration. Up-regulates Na(+) channels: SCNN1A/ENAC and SCN5A, K(+) channels: KCNA3/KV1.3, KCNE1, KCNQ1 and KCNH2/HERG, epithelial Ca(2+) channels: TRPV5 and TRPV6, chloride channel: BSND, creatine transporter: SLC6A8, Na(+)/dicarboxylate cotransporter: SLC13A2/NADC1, Na(+)-dependent phosphate cotransporter: SLC34A2/NAPI-2B, amino acid transporters: SLC1A5/ASCT2 and SLC6A19, glutamate transporters: SLC1A3/EAAT1, SLC1A6/EAAT4 and SLC1A7/EAAT5, glutamate receptors: GRIA1/GLUR1 and GRIK2/GLUR6, Na(+)/H(+) exchanger: SLC9A3/NHE3, and the Na(+)/K(+) ATPase. Plays a role in the regulation of renal tubular phosphate transport and bone density. Phosphorylates NEDD4L and GSK3B. Positively regulates ER transcription activity through phosphorylation of FLII. Negatively regulates the function of ITCH/AIP4 via its phosphorylation and thereby prevents CXCR4 from being efficiently sorted to lysosomes.
Activated by phosphorylation on Ser-486 by an unknown kinase (may be mTORC2 but not confirmed), transforming it into a substrate for PDPK1 which then phosphorylates it on Thr-320.
Cytoplasmic vesicle. Early endosome. Recycling endosome.
Note: Endosomal localization is a prerequisite for complete kinase activity. It is essential for its colocalization with the kinase responsible for phosphorylating Ser-486 thus allowing PDPK1 phosphorylation of Thr-320 resulting in complete activation of SGK3. Localized in vesicle-like structures and in the early endosome. Colocalizes with SLC9A3/NHE3 in the recycling endosomes.
Expressed in most tissues with highest levels in pancreas, kidney liver, heart and brain and lower levels in lung, placenta and skeletal muscle. Expression is higher in ER-positive breast tumors than ER-negative breast tumors.
Interacts with GSK3B and FLII. Interacts with PDPK1 in a phosphorylation-dependent manner.
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
研究领域
· Environmental Information Processing > Signal transduction > FoxO signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway. (View pathway)
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