产品: 磷酸化 DNAJC2/MPP11 (Ser49) 抗体
货号: AF7434
描述: Rabbit polyclonal antibody to Phospho-DNAJC2/MPP11 (Ser49)
应用: WB IF/ICC
反应: Human, Mouse
预测: Pig, Bovine, Horse, Sheep, Rabbit, Chicken, Xenopus
分子量: 71kDa; 72kD(Calculated).
蛋白号: Q99543
RRID: AB_2843874

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   规格 价格 库存
 100ul RMB¥ 2800 现货
 200ul RMB¥ 3800 现货

货期: 当天发货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse
预测:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Chicken(91%), Xenopus(88%)
克隆:
Polyclonal
特异性:
Phospho-DNAJC2/MPP11 (Ser49) Antibody detects endogenous levels of DNAJC2/MPP11 only when phosphorylated at Ser49.
RRID:
AB_2843874
引用格式: Affinity Biosciences Cat# AF7434, RRID:AB_2843874.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

AU020218; DnaJ (Hsp40) homolog subfamily C member 2; DnaJ homolog subfamily C member 2; Dnajc2; DNJC2_HUMAN; M phase phosphoprotein 11; M-phase phosphoprotein 11; MGC105894; MIDA1; MPHOSPH11; MPP11; OTTMUSP00000031226; ZRF1; Zrf2; ZUO1; Zuotin related factor 1; Zuotin related factor 2; Zuotin-related factor 1;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
表达:
Q99543 DNJC2_HUMAN:

Widely expressed.

序列:
MLLLPSAADGRGTAITHALTSASTLCQVEPVGRWFEAFVKRRNRNASASFQELEDKKELSEESEDEELQLEEFPMLKTLDPKDWKNQDHYAVLGLGHVRYKATQRQIKAAHKAMVLKHHPDKRKAAGEPIKEGDNDYFTCITKAYEMLSDPVKRRAFNSVDPTFDNSVPSKSEAKDNFFEVFTPVFERNSRWSNKKNVPKLGDMNSSFEDVDIFYSFWYNFDSWREFSYLDEEEKEKAECRDERRWIEKQNRATRAQRKKEEMNRIRTLVDNAYSCDPRIKKFKEEEKAKKEAEKKAKAEAKRKEQEAKEKQRQAELEAARLAKEKEEEEVRQQALLAKKEKDIQKKAIKKERQKLRNSCKTWNHFSDNEAERVKMMEEVEKLCDRLELASLQCLNETLTSCTKEVGKAALEKQIEEINEQIRKEKEEAEARMRQASKNTEKSTGGGGNGSKNWSEDDLQLLIKAVNLFPAGTNSRWEVIANYMNIHSSSGVKRTAKDVIGKAKSLQKLDPHQKDDINKKAFDKFKKEHGVVPQADNATPSERFEGPYTDFTPWTTEEQKLLEQALKTYPVNTPERWEKIAEAVPGRTKKDCMKRYKELVEMVKAKKAAQEQVLNASRAKK

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Rabbit
100
Chicken
91
Xenopus
88
Dog
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q99543 作为底物

Site PTM Type Enzyme
M1 Acetylation
K40 Ubiquitination
S47 Phosphorylation
S49 Phosphorylation
S60 Phosphorylation
S63 Phosphorylation
Y90 Phosphorylation
Y100 Phosphorylation
K112 Ubiquitination
K131 Ubiquitination
Y137 Phosphorylation
T142 Phosphorylation
K143 Ubiquitination
K153 Ubiquitination
K171 Ubiquitination
K175 Ubiquitination
T183 Phosphorylation
K249 Ubiquitination
Y274 Phosphorylation
S367 Phosphorylation
K404 Ubiquitination
K413 Ubiquitination
K504 Ubiquitination
K508 Ubiquitination
T539 Phosphorylation
Y548 Phosphorylation
K567 Ubiquitination
T573 Phosphorylation
K606 Methylation
K607 Methylation
S617 Phosphorylation

研究背景

功能:

Acts both as a chaperone in the cytosol and as a chromatin regulator in the nucleus. When cytosolic, acts as a molecular chaperone: component of the ribosome-associated complex (RAC), a complex involved in folding or maintaining nascent polypeptides in a folding-competent state. In the RAC complex, stimulates the ATPase activity of the ribosome-associated pool of Hsp70-type chaperones HSPA14 that bind to the nascent polypeptide chain. When nuclear, mediates the switching from polycomb-repressed genes to an active state: specifically recruited at histone H2A ubiquitinated at 'Lys-119' (H2AK119ub), and promotes the displacement of the polycomb PRC1 complex from chromatin, thereby facilitating transcription activation. Specifically binds DNA sequence 5'-GTCAAGC-3'.

翻译修饰:

Phosphorylated in M (mitotic) phase.

细胞定位:

Nucleus. Cytoplasm>Cytosol.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Widely expressed.

亚基结构:

Interacts (via ZRF1-UBD region) with ID1 (By similarity). Component of ribosome-associated complex (RAC), a heterodimer composed of Hsp70/DnaK-type chaperone HSPA14 and Hsp40/DnaJ-type chaperone DNAJC2.

蛋白家族:

The ZRF1-UBD region specifically recognizes and binds H2AK119ub. The ZRF1-UBD region is also involved in protein-protein interactions with other proteins, suggesting that it may be masked by some regulator, thereby preventing its association with H2AK119ub.

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