Phospho-DNAJC2/MPP11 (Ser49) Antibody - #AF7434
| 产品: | 磷酸化 DNAJC2/MPP11 (Ser49) 抗体 |
| 货号: | AF7434 |
| 描述: | Rabbit polyclonal antibody to Phospho-DNAJC2/MPP11 (Ser49) |
| 应用: | WB IF/ICC |
| 反应: | Human, Mouse |
| 预测: | Pig, Bovine, Horse, Sheep, Rabbit, Chicken, Xenopus |
| 分子量: | 71kDa; 72kD(Calculated). |
| 蛋白号: | Q99543 |
| RRID: | AB_2843874 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF7434, RRID:AB_2843874.
展开/折叠
AU020218; DnaJ (Hsp40) homolog subfamily C member 2; DnaJ homolog subfamily C member 2; Dnajc2; DNJC2_HUMAN; M phase phosphoprotein 11; M-phase phosphoprotein 11; MGC105894; MIDA1; MPHOSPH11; MPP11; OTTMUSP00000031226; ZRF1; Zrf2; ZUO1; Zuotin related factor 1; Zuotin related factor 2; Zuotin-related factor 1;
抗原和靶标
- Q99543 DNJC2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MLLLPSAADGRGTAITHALTSASTLCQVEPVGRWFEAFVKRRNRNASASFQELEDKKELSEESEDEELQLEEFPMLKTLDPKDWKNQDHYAVLGLGHVRYKATQRQIKAAHKAMVLKHHPDKRKAAGEPIKEGDNDYFTCITKAYEMLSDPVKRRAFNSVDPTFDNSVPSKSEAKDNFFEVFTPVFERNSRWSNKKNVPKLGDMNSSFEDVDIFYSFWYNFDSWREFSYLDEEEKEKAECRDERRWIEKQNRATRAQRKKEEMNRIRTLVDNAYSCDPRIKKFKEEEKAKKEAEKKAKAEAKRKEQEAKEKQRQAELEAARLAKEKEEEEVRQQALLAKKEKDIQKKAIKKERQKLRNSCKTWNHFSDNEAERVKMMEEVEKLCDRLELASLQCLNETLTSCTKEVGKAALEKQIEEINEQIRKEKEEAEARMRQASKNTEKSTGGGGNGSKNWSEDDLQLLIKAVNLFPAGTNSRWEVIANYMNIHSSSGVKRTAKDVIGKAKSLQKLDPHQKDDINKKAFDKFKKEHGVVPQADNATPSERFEGPYTDFTPWTTEEQKLLEQALKTYPVNTPERWEKIAEAVPGRTKKDCMKRYKELVEMVKAKKAAQEQVLNASRAKK
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q99543 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| K40 | Ubiquitination | Uniprot | |
| S47 | Phosphorylation | Uniprot | |
| S49 | Phosphorylation | Uniprot | |
| S60 | Phosphorylation | Uniprot | |
| S63 | Phosphorylation | Uniprot | |
| Y90 | Phosphorylation | Uniprot | |
| Y100 | Phosphorylation | Uniprot | |
| K112 | Ubiquitination | Uniprot | |
| K131 | Ubiquitination | Uniprot | |
| Y137 | Phosphorylation | Uniprot | |
| T142 | Phosphorylation | Uniprot | |
| K143 | Ubiquitination | Uniprot | |
| K153 | Ubiquitination | Uniprot | |
| K171 | Ubiquitination | Uniprot | |
| K175 | Ubiquitination | Uniprot | |
| T183 | Phosphorylation | Uniprot | |
| K249 | Ubiquitination | Uniprot | |
| Y274 | Phosphorylation | Uniprot | |
| S367 | Phosphorylation | Uniprot | |
| K404 | Ubiquitination | Uniprot | |
| K413 | Ubiquitination | Uniprot | |
| K504 | Ubiquitination | Uniprot | |
| K508 | Ubiquitination | Uniprot | |
| T539 | Phosphorylation | Uniprot | |
| Y548 | Phosphorylation | Uniprot | |
| K567 | Ubiquitination | Uniprot | |
| T573 | Phosphorylation | Uniprot | |
| K606 | Methylation | Uniprot | |
| K607 | Methylation | Uniprot | |
| S617 | Phosphorylation | Uniprot |
研究背景
Acts both as a chaperone in the cytosol and as a chromatin regulator in the nucleus. When cytosolic, acts as a molecular chaperone: component of the ribosome-associated complex (RAC), a complex involved in folding or maintaining nascent polypeptides in a folding-competent state. In the RAC complex, stimulates the ATPase activity of the ribosome-associated pool of Hsp70-type chaperones HSPA14 that bind to the nascent polypeptide chain. When nuclear, mediates the switching from polycomb-repressed genes to an active state: specifically recruited at histone H2A ubiquitinated at 'Lys-119' (H2AK119ub), and promotes the displacement of the polycomb PRC1 complex from chromatin, thereby facilitating transcription activation. Specifically binds DNA sequence 5'-GTCAAGC-3'.
Phosphorylated in M (mitotic) phase.
Nucleus. Cytoplasm>Cytosol.
Widely expressed.
Interacts (via ZRF1-UBD region) with ID1 (By similarity). Component of ribosome-associated complex (RAC), a heterodimer composed of Hsp70/DnaK-type chaperone HSPA14 and Hsp40/DnaJ-type chaperone DNAJC2.
The ZRF1-UBD region specifically recognizes and binds H2AK119ub. The ZRF1-UBD region is also involved in protein-protein interactions with other proteins, suggesting that it may be masked by some regulator, thereby preventing its association with H2AK119ub.
限制条款
产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。
产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。
Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。产品仅供科学研究使用。不用于诊断和治疗。
产品未经授权不得转售。
Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.