产品: | 磷酸化 CDCP1 (Tyr734) 抗体 |
货号: | AF7437 |
描述: | Rabbit polyclonal antibody to Phospho-CDCP1 (Tyr734) |
应用: | WB IF/ICC |
反应: | Human |
预测: | Pig, Horse, Sheep, Rabbit, Chicken, Xenopus |
分子量: | 90kDa,120kDa; 93kD(Calculated). |
蛋白号: | Q9H5V8 |
RRID: | AB_2843877 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF7437, RRID:AB_2843877.
展开/折叠
9030022E12Rik; AA409659; CD 318; CD_antigen=CD318; CD318; CD318 antigen; CDCP 1; Cdcp1; CDCP1_HUMAN; CUB domain containing protein 1; CUB domain-containing protein 1; E030027H19Rik; FLJ13772; FLJ22375; FLJ22969; Membrane glycoprotein gp140; MGC31813; OTTHUMP00000164120; OTTHUMP00000209568; RGD1305578; SIMA 135; SIMA135; Subtractive immunization M plus HEp3 associated 135 kDa protein; Subtractive immunization M plus HEp3-associated 135 kDa protein; Transmembrane and associated with src kinases; TRASK;
抗原和靶标
Highly expressed in mitotic cells with low expression during interphase. Detected at highest levels in skeletal muscle and colon with lower levels in kidney, small intestine, placenta and lung. Up-regulated in a number of human tumor cell lines, as well as in colorectal cancer, breast carcinoma and lung cancer. Also expressed in cells with phenotypes reminiscent of mesenchymal stem cells and neural stem cells.
- Q9H5V8 CDCP1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAGLNCGVSIALLGVLLLGAARLPRGAEAFEIALPRESNITVLIKLGTPTLLAKPCYIVISKRHITMLSIKSGERIVFTFSCQSPENHFVIEIQKNIDCMSGPCPFGEVQLQPSTSLLPTLNRTFIWDVKAHKSIGLELQFSIPRLRQIGPGESCPDGVTHSISGRIDATVVRIGTFCSNGTVSRIKMQEGVKMALHLPWFHPRNVSGFSIANRSSIKRLCIIESVFEGEGSATLMSANYPEGFPEDELMTWQFVVPAHLRASVSFLNFNLSNCERKEERVEYYIPGSTTNPEVFKLEDKQPGNMAGNFNLSLQGCDQDAQSPGILRLQFQVLVQHPQNESNKIYVVDLSNERAMSLTIEPRPVKQSRKFVPGCFVCLESRTCSSNLTLTSGSKHKISFLCDDLTRLWMNVEKTISCTDHRYCQRKSYSLQVPSDILHLPVELHDFSWKLLVPKDRLSLVLVPAQKLQQHTHEKPCNTSFSYLVASAIPSQDLYFGSFCPGGSIKQIQVKQNISVTLRTFAPSFQQEASRQGLTVSFIPYFKEEGVFTVTPDTKSKVYLRTPNWDRGLPSLTSVSWNISVPRDQVACLTFFKERSGVVCQTGRAFMIIQEQRTRAEEIFSLDEDVLPKPSFHHHSFWVNISNCSPTSGKQLDLLFSVTLTPRTVDLTVILIAAVGGGVLLLSALGLIICCVKKKKKKTNKGPAVGIYNDNINTEMPRQPKKFQKGRKDNDSHVYAVIEDTMVYGHLLQDSSGSFLQPEVDTYRPFQGTMGVCPPSPPTICSRAPTAKLATEEPPPRSPPESESEPYTFSHPNNGDVSSKDTDIPLLNTQEPMEPAE
种属预测
score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9H5V8 作为底物
Site | PTM Type | Enzyme | Source |
---|---|---|---|
S9 | Phosphorylation | Uniprot | |
K71 | Ubiquitination | Uniprot | |
S142 | Phosphorylation | Uniprot | |
S164 | Phosphorylation | Uniprot | |
T170 | Phosphorylation | Uniprot | |
S184 | Phosphorylation | Uniprot | |
S210 | Phosphorylation | Uniprot | |
Y283 | Phosphorylation | Uniprot | |
Y284 | Phosphorylation | Uniprot | |
K296 | Ubiquitination | Uniprot | |
K343 | Ubiquitination | Uniprot | |
S458 | Phosphorylation | Uniprot | |
K542 | Ubiquitination | Uniprot | |
K554 | Ubiquitination | Uniprot | |
S575 | Phosphorylation | Uniprot | |
S579 | Phosphorylation | Uniprot | |
T660 | Phosphorylation | Uniprot | |
K700 | Ubiquitination | Uniprot | |
Y707 | Phosphorylation | Uniprot | |
S731 | Phosphorylation | Uniprot | |
Y734 | Phosphorylation | Q05397 (PTK2) , P12931 (SRC) | Uniprot |
Y743 | Phosphorylation | P12931 (SRC) | Uniprot |
Y762 | Phosphorylation | P12931 (SRC) | Uniprot |
S775 | Phosphorylation | Uniprot | |
K787 | Ubiquitination | Uniprot | |
S797 | Phosphorylation | Uniprot | |
S801 | Phosphorylation | Uniprot | |
S803 | Phosphorylation | Uniprot | |
Y806 | Phosphorylation | Uniprot | |
T807 | Phosphorylation | Uniprot | |
S809 | Phosphorylation | Uniprot |
研究背景
May be involved in cell adhesion and cell matrix association. May play a role in the regulation of anchorage versus migration or proliferation versus differentiation via its phosphorylation. May be a novel marker for leukemia diagnosis and for immature hematopoietic stem cell subsets. Belongs to the tetraspanin web involved in tumor progression and metastasis.
Phosphorylated on tyrosine by kinases of the SRC family such as SRC and YES as well as by the protein kinase C gamma/PRKCG. Dephosphorylated by phosphotyrosine phosphatases. Also phosphorylated by suramin, a heparin analog. Tyrosine phosphorylated in response to dissociation of integrin alpha-6 beta-4 from laminin-5.
N-glycosylated.
A soluble form may also be produced by proteolytic cleavage at the cell surface (shedding). Another peptide of 80 kDa (p80) is present in cultured keratinocytes probably due to tryptic cleavage at an unidentified site on its N-terminal side. Converted to p80 by plasmin, a trypsin-like protease.
Cell membrane>Single-pass membrane protein.
Note: Shedding may also lead to a soluble peptide.
Secreted.
Highly expressed in mitotic cells with low expression during interphase. Detected at highest levels in skeletal muscle and colon with lower levels in kidney, small intestine, placenta and lung. Up-regulated in a number of human tumor cell lines, as well as in colorectal cancer, breast carcinoma and lung cancer. Also expressed in cells with phenotypes reminiscent of mesenchymal stem cells and neural stem cells.
Interacts with CDH2/N-cadherin, CDH3/P-cadherin, SDC1/syndecan-1, SDC4/syndecan-4 and the serine protease ST14/MT-SP1. Also interacts with SRC and PRKCG/protein kinase C gamma.
限制条款
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