产品: 磷酸化 Twist1 (Ser68) 抗体
货号: AF7445
描述: Rabbit polyclonal antibody to Phospho-Twist1 (Ser68)
应用: WB IF/ICC
反应: Human
预测: Pig, Bovine, Sheep, Chicken
分子量: 21kDa; 21kD(Calculated).
蛋白号: Q15672
RRID: AB_2843885

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   规格 价格 库存
 100ul RMB¥ 2800 现货
 200ul RMB¥ 3800 现货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human
预测:
Pig(100%), Bovine(100%), Sheep(100%), Chicken(83%)
克隆:
Polyclonal
特异性:
Phospho-Twist1 (Ser68) Antibody detects endogenous levels of Twist1 only when phosphorylated at Ser68.
RRID:
AB_2843885
引用格式: Affinity Biosciences Cat# AF7445, RRID:AB_2843885.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

ACS3; B-HLH DNA binding protein; bHLHa38; BPES2; BPES3; Class A basic helix-loop-helix protein 38; CRS; CRS1; CSO; H-twist; OTTHUMP00000116043; SCS; TWIST; Twist basic helix loop helix transcription factor 1; Twist family bHLH transcription factor 1; Twist homolog 1 (Drosophila); Twist homolog 1; TWIST homolog of drosophila; Twist related protein 1; Twist-related protein 1; TWIST1; TWST1_HUMAN;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
表达:
Q15672 TWST1_HUMAN:

Subset of mesodermal cells.

序列:
MMQDVSSSPVSPADDSLSNSEEEPDRQQPPSGKRGGRKRRSSRRSAGGGAGPGGAAGGGVGGGDEPGSPAQGKRGKKSAGCGGGGGAGGGGGSSSGGGSPQSYEELQTQRVMANVRERQRTQSLNEAFAALRKIIPTLPSDKLSKIQTLKLAARYIDFLYQVLQSDELDSKMASCSYVAHERLSYAFSVWRMEGAWSMSASH

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Chicken
83
Horse
0
Dog
0
Xenopus
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q15672 作为底物

Site PTM Type Enzyme
S6 Phosphorylation P28482 (MAPK1)
S18 Phosphorylation P68400 (CSNK2A1)
S20 Phosphorylation P68400 (CSNK2A1)
K38 Ubiquitination
S42 Phosphorylation P31749 (AKT1)
S45 Phosphorylation
S68 Phosphorylation Q15759 (MAPK11) , P45983 (MAPK8) , Q16539 (MAPK14) , P28482 (MAPK1) , P27361 (MAPK3)
K73 Acetylation
S102 Phosphorylation
Y103 Phosphorylation
T121 Phosphorylation
S123 Phosphorylation P31749 (AKT1)
T137 Phosphorylation
K142 Ubiquitination
S144 Phosphorylation
K145 Ubiquitination

研究背景

功能:

Acts as a transcriptional regulator. Inhibits myogenesis by sequestrating E proteins, inhibiting trans-activation by MEF2, and inhibiting DNA-binding by MYOD1 through physical interaction. This interaction probably involves the basic domains of both proteins. Also represses expression of proinflammatory cytokines such as TNFA and IL1B. Regulates cranial suture patterning and fusion. Activates transcription as a heterodimer with E proteins. Regulates gene expression differentially, depending on dimer composition. Homodimers induce expression of FGFR2 and POSTN while heterodimers repress FGFR2 and POSTN expression and induce THBS1 expression. Heterodimerization is also required for osteoblast differentiation. Represses the activity of the circadian transcriptional activator: NPAS2-ARNTL/BMAL1 heterodimer (By similarity).

细胞定位:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Subset of mesodermal cells.

亚基结构:

Efficient DNA binding requires dimerization with another bHLH protein. Homodimer or heterodimer with E proteins such as TCF3. ID1 binds preferentially to TCF3 but does not interact efficiently with TWIST1 so ID1 levels control the amount of TCF3 available to dimerize with TWIST1 and thus determine the type of dimer formed (By similarity).

研究领域

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

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