| 产品: | 磷酸化 CDC20 (Thr70) 抗体 |
| 货号: | AF7446 |
| 描述: | Rabbit polyclonal antibody to Phospho-CDC20 (Thr70) |
| 应用: | WB |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Bovine, Horse, Sheep, Rabbit, Dog |
| 分子量: | 50KD; 55kD(Calculated). |
| 蛋白号: | Q12834 |
| RRID: | AB_2843886 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF7446, RRID:AB_2843886.
展开/折叠
bA276H19.3; Cdc 20; CDC20; CDC20 cell division cycle 20 homolog; CDC20_HUMAN; CDC20A; Cell division cycle 20; Cell division cycle 20 homolog (S. cerevisiae); Cell division cycle 20 homolog; Cell division cycle protein 20 homolog; fizzy; MGC102824; p55CDC;
抗原和靶标
- Q12834 CDC20_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAQFAFESDLHSLLQLDAPIPNAPPARWQRKAKEAAGPAPSPMRAANRSHSAGRTPGRTPGKSSSKVQTTPSKPGGDRYIPHRSAAQMEVASFLLSKENQPENSQTPTKKEHQKAWALNLNGFDVEEAKILRLSGKPQNAPEGYQNRLKVLYSQKATPGSSRKTCRYIPSLPDRILDAPEIRNDYYLNLVDWSSGNVLAVALDNSVYLWSASSGDILQLLQMEQPGEYISSVAWIKEGNYLAVGTSSAEVQLWDVQQQKRLRNMTSHSARVGSLSWNSYILSSGSRSGHIHHHDVRVAEHHVATLSGHSQEVCGLRWAPDGRHLASGGNDNLVNVWPSAPGEGGWVPLQTFTQHQGAVKAVAWCPWQSNVLATGGGTSDRHIRIWNVCSGACLSAVDAHSQVCSILWSPHYKELISGHGFAQNQLVIWKYPTMAKVAELKGHTSRVLSLTMSPDGATVASAAADETLRLWRCFELDPARRREREKASAAKSSLIHQGIR
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q12834 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Ubiquitination | Uniprot | ||
| K33 | Methylation | Uniprot | |
| S41 | Phosphorylation | PR:O43683 (hBUB1) , O43683 (BUB1) | Uniprot |
| S49 | Phosphorylation | Uniprot | |
| S51 | Phosphorylation | Uniprot | |
| T55 | Phosphorylation | Uniprot | |
| T59 | Phosphorylation | Uniprot | |
| K62 | Ubiquitination | Uniprot | |
| S63 | Phosphorylation | Uniprot | |
| S64 | Phosphorylation | Uniprot | |
| K66 | Acetylation | Uniprot | |
| K66 | Ubiquitination | Uniprot | |
| T69 | Phosphorylation | Uniprot | |
| T70 | Phosphorylation | Uniprot | |
| S72 | Phosphorylation | PR:O43683 (hBUB1) , O43683 (BUB1) | Uniprot |
| K73 | Ubiquitination | Uniprot | |
| Y79 | Phosphorylation | Uniprot | |
| S84 | Phosphorylation | Uniprot | |
| S92 | Phosphorylation | O43683 (BUB1) , PR:O43683 (hBUB1) | Uniprot |
| S96 | Phosphorylation | Uniprot | |
| K97 | Ubiquitination | Uniprot | |
| T106 | Phosphorylation | Uniprot | |
| K109 | Sumoylation | Uniprot | |
| K109 | Ubiquitination | Uniprot | |
| K114 | Ubiquitination | Uniprot | |
| K129 | Ubiquitination | Uniprot | |
| S134 | Phosphorylation | Uniprot | |
| K136 | Ubiquitination | Uniprot | |
| K149 | Ubiquitination | Uniprot | |
| Y152 | Phosphorylation | Uniprot | |
| S153 | Phosphorylation | O43683 (BUB1) , PR:O43683 (hBUB1) | Uniprot |
| K155 | Ubiquitination | Uniprot | |
| T157 | Phosphorylation | O43683 (BUB1) , PR:O43683 (hBUB1) | Uniprot |
| S160 | Phosphorylation | Uniprot | |
| S161 | Phosphorylation | PR:O43683 (hBUB1) , O43683 (BUB1) | Uniprot |
| T164 | Phosphorylation | Uniprot | |
| S170 | Phosphorylation | P53350 (PLK1) | Uniprot |
| R174 | Methylation | Uniprot | |
| K259 | Ubiquitination | Uniprot | |
| S285 | Phosphorylation | Uniprot | |
| K435 | Ubiquitination | Uniprot | |
| K440 | Ubiquitination | Uniprot | |
| S448 | Phosphorylation | Uniprot | |
| T457 | Phosphorylation | Uniprot | |
| T466 | Phosphorylation | Uniprot | |
| K485 | Ubiquitination | Uniprot | |
| S487 | Phosphorylation | Uniprot | |
| K490 | Sumoylation | Uniprot | |
| K490 | Ubiquitination | Uniprot | |
| S492 | Phosphorylation | Uniprot |
研究背景
Required for full ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) and may confer substrate specificity upon the complex. Is regulated by MAD2L1: in metaphase the MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the CDC20-APC/C binary complex is active in degrading substrates. The CDC20-APC/C complex positively regulates the formation of synaptic vesicle clustering at active zone to the presynaptic membrane in postmitotic neurons. CDC20-APC/C-induced degradation of NEUROD2 induces presynaptic differentiation.
Acetylated. Deacetylated at Lys-66 by SIRT2; deacetylation enhances the interaction of CDC20 with CDC27, leading to activation of anaphase promoting complex/cyclosome (APC/C).
Phosphorylated during mitosis, probably by maturation promoting factor (MPF). Phosphorylated by BUB1 at Ser-41; Ser-72; Ser-92; Ser-153; Thr-157 and Ser-161. Phosphorylated by NEK2.
Dephosphorylated by CTDP1.
Ubiquitinated and degraded by the proteasome during spindle assembly checkpoint. Deubiquitinated by USP44, leading to stabilize the MAD2L1-CDC20-APC/C ternary complex, thereby preventing premature activation of the APC/C. Ubiquitinated at Lys-490 during prometaphase. Ubiquitination at Lys-485 and Lys-490 has no effect on its ability to bind the APC/C complex.
Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Cytoplasm>Cytoskeleton>Spindle pole.
Found in a complex with CDC20, CDC27, SPATC1 and TUBG1. Interacts with NEUROD2 and SPATC1 (By similarity). Interacts with MAD2L1 and BUB1B. The phosphorylated form interacts with APC/C. Interacts with NINL. May interact with MAD2L2. Interacts with CDK5RAP2 and SIRT2. Interacts with isoform 1 of NEK2. Interacts with HSF1 (via phosphorylated form); this interaction occurs in mitosis in a MAD2L1-dependent manner and prevents PLK1-stimulated degradation of HSF1 by blocking the recruitment of the SCF(BTRC) ubiquitin ligase complex. Interacts (via the N-terminal substrate-binding domain) with FBXO5 (By similarity).
Belongs to the WD repeat CDC20/Fizzy family.
研究领域
· Cellular Processes > Cell growth and death > Cell cycle. (View pathway)
· Cellular Processes > Cell growth and death > Oocyte meiosis. (View pathway)
· Genetic Information Processing > Folding, sorting and degradation > Ubiquitin mediated proteolysis. (View pathway)
· Human Diseases > Infectious diseases: Viral > HTLV-I infection.
· Human Diseases > Cancers: Overview > Viral carcinogenesis.
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