Phospho-Cyclin D3 (Thr283) Antibody - #AF3251

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产品: 磷酸化 Cyclin D3 (Thr283) 抗体
货号: AF3251
描述: Rabbit polyclonal antibody to Phospho-Cyclin D3 (Thr283)
应用: WB IHC IF/ICC
文献验证: WB
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
分子量: 31kDa; 33kD(Calculated).
蛋白号: P30281
RRID: AB_2834677

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实验方案
WB Handbook
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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
预测:
Pig(100%), Bovine(%), Horse(%), Sheep(%), Rabbit(%), Dog(%), Chicken(%)
克隆:
Polyclonal
特异性:
Phospho-Cyclin D3 (Thr283) Antibody detects endogenous levels of Cyclin D3 only when phosphorylated at Threonine 283.
RRID:
AB_2834677
引用格式: Affinity Biosciences Cat# AF3251, RRID:AB_2834677.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

CCND 3; Ccnd3; CCND3_HUMAN; CyclinD3; D3 type cyclin; G1 S specific cyclin D3; G1/S specific cyclin D3; G1/S-specific cyclin-D3;

抗原和靶标

免疫原:

A synthesized peptide derived from human Cyclin D3 around the phosphorylation site of Thr283.

Uniprot:

P30281(CCND3_HUMAN) >>Visit HPA database.

基因/基因ID:
CCND3(896)
描述:
CCND3 Regulatory component of the cyclin D3-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition.
序列:
MELLCCEGTRHAPRAGPDPRLLGDQRVLQSLLRLEERYVPRASYFQCVQREIKPHMRKMLAYWMLEVCEEQRCEEEVFPLAMNYLDRYLSCVPTRKAQLQLLGAVCMLLASKLRETTPLTIEKLCIYTDHAVSPRQLRDWEVLVLGKLKWDLAAVIAHDFLAFILHRLSLPRDRQALVKKHAQTFLALCATDYTFAMYPPSMIATGSIGAAVQGLGACSMSGDELTELLAGITGTEVDCLRACQEQIEAALRESLREASQTSSSPAPKAPRGSSSQGPSQTSTPTDVTAIHL

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

功能:

Regulatory component of the cyclin D3-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D3/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.

翻译修饰:

Polyubiquitinated by the SCF(FBXL2) complex, leading to proteasomal degradation.

细胞定位:

Nucleus. Cytoplasm. Membrane.
Note: Cyclin D-CDK4 complexes accumulate at the nuclear membrane and are then translocated to the nucleus through interaction with KIP/CIP family members.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
蛋白家族:

Belongs to the cyclin family. Cyclin D subfamily.

研究领域

· Cellular Processes > Cell growth and death > Cell cycle.   (View pathway)

· Cellular Processes > Cell growth and death > p53 signaling pathway.   (View pathway)

· Cellular Processes > Cell growth and death > Cellular senescence.   (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Focal adhesion.   (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Wnt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Hippo signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Viral > Measles.

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

文献引用

1). Mechanism of D-type cyclin recognition by the AMBRA1 E3 ligase receptor. Science advances, 2025 (PubMed: 40408472) [IF=11.7]

Application: WB    Species: human    Sample:

Fig. 5. AMBRA1 mediates the ubiquitination of D-type cyclins. (A) Ubiquitination assay of AMBRA1WD40 mediated the polyubiquitination of TSF–cyclin D1, T286A mutant, and C-terminal truncation. The experiment was repeated independently three times with similar results. (B) Ubiquitination assay of AMBRA1WD40 mediated the polyubiquitination of TSF–cyclin D2/cyclin D2T280A and TSF–cyclin D3/cyclin D3T283A. The experiment was repeated independently three times with similar results. (C) In vitro pull-down experiment of GST–cyclin D1 with MBP-AMBRA1FL-His and H75A/R96A/W99A mutant. The cells were cotransfected with GST–cyclin D1, CDK4EE-His6, His6-DDB1, and MBP-AMBRA1FL-His or MBP-AMBRA1H75A/R96A/W99A-His mutant. The experiment was repeated at least three times and visualized by Western blotting. (D) Ubiquitination assay of AMBRA1FL mediated the polyubiquitination of TSF–D-type cyclins and threonine phosphorylation site mutants. The experiment was repeated independently three times with similar results. (E) Ubiquitination assay of AMBRA1FL mediated the polyubiquitination of CDK4 was detected. The experiment was repeated independently three times with similar results. (F) Ubiquitination assay of AMBRA1FL mediated the polyubiquitination of GST–cyclin D1 C terminus, the hemagglutinin (HA)–ubiquitin, and K269R mutant as negative control. The experiment was repeated independently three times with similar results. (G) Ubiquitination assay of AMBRA1 mediated the polyubiquitination of GST–cyclin D1 C termini. Asterisk indicates the nonspecific band. The experiment was repeated independently three times with similar results. (H) Ubiquitination assay of AMBRA1WD40 WT and key binding site mutants mediated the polyubiquitination of cyclin D1. The results were analyzed by Western blotting. The experiment was repeated independently three times with similar results.

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