Phospho-ADD1 (Ser726) Antibody - #AF3276

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产品: 磷酸化 ADD1 (Ser726) 抗体
货号: AF3276
描述: Rabbit polyclonal antibody to Phospho-ADD1 (Ser726)
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Zebrafish, Bovine, Horse, Dog, Xenopus
分子量: 80kDa; 81kD(Calculated).
蛋白号: P35611 | P35612
RRID: AB_2834699

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MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Zebrafish(100%), Bovine(100%), Horse(100%), Dog(100%), Xenopus(100%)
克隆:
Polyclonal
特异性:
Phospho-ADD1 (Ser726) Antibody detects endogenous levels of ADD1 only when phosphorylated at Serine 726.
RRID:
AB_2834699
引用格式: Affinity Biosciences Cat# AF3276, RRID:AB_2834699.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

ADD A; ADD1; ADDA; ADDA_HUMAN; Adducin 1 (alpha); Alpha adducin; Alpha-adducin; Erythrocyte adducin alpha subunit; Erythrocyte adducin subunit alpha; MGC3339; MGC44427; OTTHUMP00000151163; OTTHUMP00000151164; OTTHUMP00000151165; OTTHUMP00000218664; OTTHUMP00000218665; ADD1; ADD2; ADD3; ADDA; ADDB; ADDL; Adducin 1 (alpha); Adducin 1; Adducin 2 (beta); Adducin 2; Adducin 3 (gamma); Adducin 3; Adducin like; Alpha adducin; Beta adducin;

抗原和靶标

免疫原:
Uniprot:

P35611(ADDA_HUMAN) >>Visit HPA database.

P35612(ADDB_HUMAN) >>Visit HPA database.

基因/基因ID:
ADD1(118) | ADD2(119)
表达:
P35611 ADDA_HUMAN:

Expressed in all tissues. Found in much higher levels in reticulocytes than the beta subunit.

P35612 ADDB_HUMAN:

Expressed mainly in brain, spleen, kidney cortex and medulla, and heart. Also expressed in human umbilical vein endothelial cells, human vascular smooth muscle cells, kidney tubular cells and K-562 cell line.

描述:
ADD2 a cytoskeletal protein that promotes the assembly of the spectrin-actin network. Adducin is a heterodimeric protein that consists of related subunits. Alpha- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region.
序列:
MNGDSRAAVVTSPPPTTAPHKERYFDRVDENNPEYLRERNMAPDLRQDFNMMEQKKRVSMILQSPAFCEELESMIQEQFKKGKNPTGLLALQQIADFMTTNVPNVYPAAPQGGMAALNMSLGMVTPVNDLRGSDSIAYDKGEKLLRCKLAAFYRLADLFGWSQLIYNHITTRVNSEQEHFLIVPFGLLYSEVTASSLVKINLQGDIVDRGSTNLGVNQAGFTLHSAIYAARPDVKCVVHIHTPAGAAVSAMKCGLLPISPEALSLGEVAYHDYHGILVDEEEKVLIQKNLGPKSKVLILRNHGLVSVGESVEEAFYYIHNLVVACEIQVRTLASAGGPDNLVLLNPEKYKAKSRSPGSPVGEGTGSPPKWQIGEQEFEALMRMLDNLGYRTGYPYRYPALREKSKKYSDVEVPASVTGYSFASDGDSGTCSPLRHSFQKQQREKTRWLNSGRGDEASEEGQNGSSPKSKTKWTKEDGHRTSTSAVPNLFVPLNTNPKEVQEMRNKIREQNLQDIKTAGPQSQVLCGVVMDRSLVQGELVTASKAIIEKEYQPHVIVSTTGPNPFTTLTDRELEEYRREVERKQKGSEENLDEAREQKEKSPPDQPAVPHPPPSTPIKLEEDLVPEPTTGDDSDAATFKPTLPDLSPDEPSEALGFPMLEKEEEAHRPPSPTEAPTEASPEPAPDPAPVAEEAAPSAVEEGAAADPGSDGSPGKSPSKKKKKFRTPSFLKKSKKKSDS

MSEETVPEAASPPPPQGQPYFDRFSEDDPEYMRLRNRAADLRQDFNLMEQKKRVTMILQSPSFREELEGLIQEQMKKGNNSSNIWALRQIADFMASTSHAVFPTSSMNVSMMTPINDLHTADSLNLAKGERLMRCKISSVYRLLDLYGWAQLSDTYVTLRVSKEQDHFLISPKGVSCSEVTASSLIKVNILGEVVEKGSSCFPVDTTGFCLHSAIYAARPDVRCIIHLHTPATAAVSAMKWGLLPVSHNALLVGDMAYYDFNGEMEQEADRINLQKCLGPTCKILVLRNHGVVALGDTVEEAFYKIFHLQAACEIQVSALSSAGGVENLILLEQEKHRPHEVGSVQWAGSTFGPMQKSRLGEHEFEALMRMLDNLGYRTGYTYRHPFVQEKTKHKSEVEIPATVTAFVFEEDGAPVPALRQHAQKQQKEKTRWLNTPNTYLRVNVADEVQRSMGSPRPKTTWMKADEVEKSSSGMPIRIENPNQFVPLYTDPQEVLEMRNKIREQNRQDVKSAGPQSQLLASVIAEKSRSPSTESQLMSKGDEDTKDDSEETVPNPFSQLTDQELEEYKKEVERKKLELDGEKETAPEEPGSPAKSAPASPVQSPAKEAETKSPLVSPSKSLEEGTKKTETSKAATTEPETTQPEGVVVNGREEEQTAEEILSKGLSQMTTSADTDVDTSKDKTESVTSGPMSPEGSPSKSPSKKKKKFRTPSFLKKSKKKEKVES

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Horse
100
Bovine
100
Dog
100
Xenopus
100
Zebrafish
100
Pig
0
Sheep
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P35611/P35612 作为底物

Site PTM Type Enzyme
M1 Acetylation
S5 Phosphorylation
T11 Phosphorylation
S12 Phosphorylation P06493 (CDK1)
T16 Phosphorylation
K21 Ubiquitination
Y24 Phosphorylation
R27 Methylation
Y35 Phosphorylation
K55 Ubiquitination
S59 Phosphorylation
S64 Phosphorylation
K140 Acetylation
K140 Ubiquitination
K143 Ubiquitination
K148 Ubiquitination
K288 Ubiquitination
T331 Phosphorylation
S334 Phosphorylation
K348 Ubiquitination
S353 Phosphorylation
S355 Phosphorylation P06493 (CDK1)
S358 Phosphorylation
T364 Phosphorylation
S366 Phosphorylation
Y393 Phosphorylation
Y395 Phosphorylation
K406 Ubiquitination
S408 Phosphorylation P17612 (PRKACA)
S423 Phosphorylation
S427 Phosphorylation
T429 Phosphorylation
S431 Phosphorylation
S436 Phosphorylation P17612 (PRKACA)
K439 Ubiquitination
T445 Phosphorylation Q9Y5S2 (CDC42BPB) , Q13464 (ROCK1) , O75116 (ROCK2)
S457 Phosphorylation
S464 Phosphorylation
S465 Phosphorylation
T480 Phosphorylation Q13464 (ROCK1) , O75116 (ROCK2)
S481 Phosphorylation P17612 (PRKACA)
T482 Phosphorylation
S483 Phosphorylation
K515 Ubiquitination
C525 S-Nitrosylation
R531 Methylation
S532 Phosphorylation
Y550 Phosphorylation
T559 Phosphorylation
Y575 Phosphorylation
S586 Phosphorylation
S600 Phosphorylation
S613 Phosphorylation P24941 (CDK2)
T614 Phosphorylation P24941 (CDK2)
S645 Phosphorylation
S669 Phosphorylation
T671 Phosphorylation
S678 Phosphorylation
S707 Phosphorylation
S710 Phosphorylation
S714 Phosphorylation
S716 Phosphorylation P17252 (PRKCA)
T724 Phosphorylation
S726 Phosphorylation Q05513 (PRKCZ) , O14757 (CHEK1) , P17252 (PRKCA) , Q05655 (PRKCD) , P17612 (PRKACA)
S731 Phosphorylation
S735 Phosphorylation
S737 Phosphorylation
Site PTM Type Enzyme
S2 Phosphorylation
T5 Phosphorylation
S11 Phosphorylation
Y31 Phosphorylation
T55 Phosphorylation
S60 Phosphorylation
S62 Phosphorylation
Y377 Phosphorylation
S396 Phosphorylation
Y440 Phosphorylation
S455 Phosphorylation
K464 Acetylation
S472 Phosphorylation
Y489 Phosphorylation P06241 (FYN)
S512 Phosphorylation
S522 Phosphorylation
S528 Phosphorylation
S530 Phosphorylation
S532 Phosphorylation
T533 Phosphorylation
S535 Phosphorylation
K576 Acetylation
K583 Acetylation
T585 Phosphorylation
S592 Phosphorylation
K595 Acetylation
S596 Phosphorylation
S600 Phosphorylation
S604 Phosphorylation Q00535 (CDK5)
T611 Phosphorylation
S613 Phosphorylation P49841 (GSK3B)
S617 Phosphorylation Q00535 (CDK5)
S619 Phosphorylation
S621 Phosphorylation
T626 Phosphorylation
T631 Phosphorylation
S632 Phosphorylation
T675 Phosphorylation
K681 Acetylation
K683 Acetylation
T684 Phosphorylation
S693 Phosphorylation P49841 (GSK3B)
S697 Phosphorylation
S699 Phosphorylation
S701 Phosphorylation Q00535 (CDK5)
S703 Phosphorylation
T711 Phosphorylation
S713 Phosphorylation O14757 (CHEK1) , P17612 (PRKACA) , Q05513 (PRKCZ) , P17252 (PRKCA)
S718 Phosphorylation
S726 Phosphorylation P17252 (PRKCA)

研究背景

功能:

Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin.

细胞定位:

Cytoplasm>Cytoskeleton. Cell membrane>Peripheral membrane protein>Cytoplasmic side.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed in all tissues. Found in much higher levels in reticulocytes than the beta subunit.

亚基结构:

Heterodimer of an alpha and a beta subunit or an alpha and a gamma subunit.

蛋白家族:

Each subunit is comprised of three regions: a NH2-terminal protease-resistant globular head region, a short connecting subdomain, and a protease-sensitive tail region.

Belongs to the aldolase class II family. Adducin subfamily.

功能:

Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to the erythrocyte membrane receptor SLC2A1/GLUT1 and may therefore provide a link between the spectrin cytoskeleton to the plasma membrane. Binds to calmodulin. Calmodulin binds preferentially to the beta subunit.

翻译修饰:

The N-terminus is blocked.

细胞定位:

Cytoplasm>Cytoskeleton. Cell membrane>Peripheral membrane protein>Cytoplasmic side.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed mainly in brain, spleen, kidney cortex and medulla, and heart. Also expressed in human umbilical vein endothelial cells, human vascular smooth muscle cells, kidney tubular cells and K-562 cell line.

亚基结构:

Heterodimer of an alpha and a beta subunit. Found in a complex with ADD2, DMTN and SLC2A1. Interacts with SLC2A1.

蛋白家族:

Each subunit is comprised of three regions: a NH2-terminal protease-resistant globular head region, a short connecting subdomain, and a protease-sensitive tail region.

Belongs to the aldolase class II family. Adducin subfamily.

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