Phospho-HNF4 alpha (Ser313) Antibody - #AF3297
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF3297, RRID:AB_2834716.
展开/折叠
FLJ39654; FRTS4; Hepatic nuclear factor 4 alpha; Hepatocyte nuclear factor 4 alpha; Hepatocyte nuclear factor 4; Hepatocyte nuclear factor 4-alpha; HNF 4 alpha; HNF 4; HNF-4-alpha; HNF4; HNF4A; HNF4A_HUMAN; HNF4a7; HNF4a8; HNF4a9; Hnf4alpha; HNF4alpha10/11/12; MODY 1; MODY; MODY1; NR2A1; NR2A21; Nuclear receptor subfamily 2 group A member 1; OTTHUMP00000031060; OTTHUMP00000031062; TCF 14; TCF; TCF-14; TCF14; Tcf4; Transcription factor 14, hepatic nuclear factor; Transcription factor 14; Transcription factor HNF 4; Transcription factor HNF-4; Transcription factor HNF4;
抗原和靶标
- P41235 HNF4A_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MRLSKTLVDMDMADYSAALDPAYTTLEFENVQVLTMGNDTSPSEGTNLNAPNSLGVSALCAICGDRATGKHYGASSCDGCKGFFRRSVRKNHMYSCRFSRQCVVDKDKRNQCRYCRLKKCFRAGMKKEAVQNERDRISTRRSSYEDSSLPSINALLQAEVLSRQITSPVSGINGDIRAKKIASIADVCESMKEQLLVLVEWAKYIPAFCELPLDDQVALLRAHAGEHLLLGATKRSMVFKDVLLLGNDYIVPRHCPELAEMSRVSIRILDELVLPFQELQIDDNEYAYLKAIIFFDPDAKGLSDPGKIKRLRSQVQVSLEDYINDRQYDSRGRFGELLLLLPTLQSITWQMIEQIQFIKLFGMAKIDNLLQEMLLGGSPSDAPHAHHPLHPHLMQEHMGTNVIVANTMPTHLSNGQMCEWPRPRGQAATPETPQPSPPGGSGSEPYKLLPGAVATIVKPLSAIPQPTITKQEVI
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P41235 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y23 | Phosphorylation | P12931 (SRC) | Uniprot |
| Y72 | Phosphorylation | Uniprot | |
| S75 | Phosphorylation | Uniprot | |
| S76 | Phosphorylation | Uniprot | |
| S95 | Phosphorylation | Uniprot | |
| S99 | Phosphorylation | Uniprot | |
| K106 | Acetylation | Uniprot | |
| K108 | Acetylation | Uniprot | |
| K126 | Acetylation | Uniprot | |
| K127 | Acetylation | Uniprot | |
| S138 | Phosphorylation | Uniprot | |
| T139 | Phosphorylation | Uniprot | |
| S142 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| S143 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| Y144 | Phosphorylation | Uniprot | |
| S147 | Phosphorylation | Uniprot | |
| S148 | Phosphorylation | Uniprot | |
| S151 | Phosphorylation | Uniprot | |
| T166 | Phosphorylation | Uniprot | |
| S167 | Phosphorylation | Q16539 (MAPK14) , Q15759 (MAPK11) | Uniprot |
| S170 | Phosphorylation | Uniprot | |
| S190 | Phosphorylation | Uniprot | |
| K234 | Ubiquitination | Uniprot | |
| S262 | Phosphorylation | Uniprot | |
| S265 | Phosphorylation | Uniprot | |
| Y286 | Phosphorylation | P12931 (SRC) | Uniprot |
| Y288 | Phosphorylation | P12931 (SRC) | Uniprot |
| S303 | Phosphorylation | Q13131 (PRKAA1) | Uniprot |
| K307 | Ubiquitination | Uniprot | |
| K309 | Ubiquitination | Uniprot | |
| S313 | Phosphorylation | Q13131 (PRKAA1) | Uniprot |
| S318 | Phosphorylation | Uniprot | |
| S378 | Phosphorylation | Uniprot | |
| T429 | Phosphorylation | Uniprot | |
| T432 | Phosphorylation | Uniprot | |
| S436 | Phosphorylation | Uniprot | |
| S441 | Phosphorylation | Uniprot | |
| S443 | Phosphorylation | Uniprot | |
| T455 | Phosphorylation | Uniprot | |
| K458 | Acetylation | Uniprot | |
| S461 | Phosphorylation | Uniprot | |
| T467 | Phosphorylation | Uniprot | |
| T469 | Phosphorylation | Uniprot |
研究背景
Transcriptional regulator which controls the expression of hepatic genes during the transition of endodermal cells to hepatic progenitor cells, facilitating the recruitment of RNA pol II to the promoters of target genes. Activates the transcription of CYP2C38 (By similarity). Represses the CLOCK-ARNTL/BMAL1 transcriptional activity and is essential for circadian rhythm maintenance and period regulation in the liver and colon cells.
Phosphorylated on tyrosine residue(s); phosphorylation is important for its DNA-binding activity. Phosphorylation may directly or indirectly play a regulatory role in the subnuclear distribution. Phosphorylation at Ser-313 by AMPK reduces the ability to form homodimers and bind DNA.
Acetylation at Lys-458 lowers transcriptional activation by about two-fold.
Nucleus.
Homodimerization is required for HNF4-alpha to bind to its recognition site. Interacts with CLOCK, ARNTL, CRY1, CRY2, PER1 and PER2. Interacts with NR0B2/SHP; the resulting heterodimer is transcriptionnally inactive. Interacts with DDX3X; this interaction disrupts the interaction between HNF4 and NR0B2 that forms inactive heterodimers and enhances the formation of active HNF4 homodimers.
The 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.
Belongs to the nuclear hormone receptor family. NR2 subfamily.
研究领域
· Environmental Information Processing > Signal transduction > AMPK signaling pathway. (View pathway)
· Human Diseases > Endocrine and metabolic diseases > Maturity onset diabetes of the young.
限制条款
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