产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF4303, RRID:AB_2844382.
展开/折叠
coiled-coil-containing protein kinase 1; coiled-coil-containing protein kinase I; MGC131603; MGC43611; p160 Rhoassociated coiled coil-forming protein kinase; p160 ROCK-1; p160 ROCK1; p160ROCK; PRO0435; Renal carcinoma antigen NY REN 35; Renal carcinoma antigen NY-REN-35; Rho associated coiled coil containing protein kinase 1; Rho associated protein kinase 1; Rho kinase; Rho-alpha kinase; Rho-associated; Rho-associated protein kinase 1; ROCK I; ROCK-I; ROCK1; ROCK1_HUMAN; Rok; rokalpha;
抗原和靶标
- Q13464 ROCK1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSTGDSFETRFEKMDNLLRDPKSEVNSDCLLDGLDALVYDLDFPALRKNKNIDNFLSRYKDTINKIRDLRMKAEDYEVVKVIGRGAFGEVQLVRHKSTRKVYAMKLLSKFEMIKRSDSAFFWEERDIMAFANSPWVVQLFYAFQDDRYLYMVMEYMPGGDLVNLMSNYDVPEKWARFYTAEVVLALDAIHSMGFIHRDVKPDNMLLDKSGHLKLADFGTCMKMNKEGMVRCDTAVGTPDYISPEVLKSQGGDGYYGRECDWWSVGVFLYEMLVGDTPFYADSLVGTYSKIMNHKNSLTFPDDNDISKEAKNLICAFLTDREVRLGRNGVEEIKRHLFFKNDQWAWETLRDTVAPVVPDLSSDIDTSNFDDLEEDKGEEETFPIPKAFVGNQLPFVGFTYYSNRRYLSSANPNDNRTSSNADKSLQESLQKTIYKLEEQLHNEMQLKDEMEQKCRTSNIKLDKIMKELDEEGNQRRNLESTVSQIEKEKMLLQHRINEYQRKAEQENEKRRNVENEVSTLKDQLEDLKKVSQNSQLANEKLSQLQKQLEEANDLLRTESDTAVRLRKSHTEMSKSISQLESLNRELQERNRILENSKSQTDKDYYQLQAILEAERRDRGHDSEMIGDLQARITSLQEEVKHLKHNLEKVEGERKEAQDMLNHSEKEKNNLEIDLNYKLKSLQQRLEQEVNEHKVTKARLTDKHQSIEEAKSVAMCEMEKKLKEEREAREKAENRVVQIEKQCSMLDVDLKQSQQKLEHLTGNKERMEDEVKNLTLQLEQESNKRLLLQNELKTQAFEADNLKGLEKQMKQEINTLLEAKRLLEFELAQLTKQYRGNEGQMRELQDQLEAEQYFSTLYKTQVKELKEEIEEKNRENLKKIQELQNEKETLATQLDLAETKAESEQLARGLLEEQYFELTQESKKAASRNRQEITDKDHTVSRLEEANSMLTKDIEILRRENEELTEKMKKAEEEYKLEKEEEISNLKAAFEKNINTERTLKTQAVNKLAEIMNRKDFKIDRKKANTQDLRKKEKENRKLQLELNQEREKFNQMVVKHQKELNDMQAQLVEECAHRNELQMQLASKESDIEQLRAKLLDLSDSTSVASFPSADETDGNLPESRIEGWLSVPNRGNIKRYGWKKQYVVVSSKKILFYNDEQDKEQSNPSMVLDIDKLFHVRPVTQGDVYRAETEEIPKIFQILYANEGECRKDVEMEPVQQAEKTNFQNHKGHEFIPTLYHFPANCDACAKPLWHVFKPPPALECRRCHVKCHRDHLDKKEDLICPCKVSYDVTSARDMLLLACSQDEQKKWVTHLVKKIPKNPPSGFVRASPRTLSTRSTANQSFRKVVKNTSGKTS
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q13464 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Acetylation | Uniprot | |
| S2 | Phosphorylation | Uniprot | |
| T3 | Phosphorylation | Uniprot | |
| K13 | Ubiquitination | Uniprot | |
| K65 | Ubiquitination | Uniprot | |
| K80 | Ubiquitination | Uniprot | |
| K109 | Ubiquitination | Uniprot | |
| K200 | Ubiquitination | Uniprot | |
| K225 | Ubiquitination | Uniprot | |
| Y255 | Phosphorylation | Uniprot | |
| S282 | Phosphorylation | Uniprot | |
| K294 | Ubiquitination | Uniprot | |
| K307 | Ubiquitination | Uniprot | |
| K339 | Ubiquitination | Uniprot | |
| T398 | Phosphorylation | Uniprot | |
| Y405 | Phosphorylation | Uniprot | |
| S407 | Phosphorylation | Uniprot | |
| K430 | Ubiquitination | Uniprot | |
| T455 | Phosphorylation | Uniprot | |
| S456 | Phosphorylation | Uniprot | |
| S533 | Phosphorylation | Uniprot | |
| K539 | Ubiquitination | Uniprot | |
| S567 | Phosphorylation | Uniprot | |
| T569 | Phosphorylation | Uniprot | |
| S572 | Phosphorylation | Uniprot | |
| S574 | Phosphorylation | Uniprot | |
| S576 | Phosphorylation | Uniprot | |
| Y603 | Phosphorylation | Uniprot | |
| Y604 | Phosphorylation | Uniprot | |
| K647 | Acetylation | Uniprot | |
| S679 | Phosphorylation | Uniprot | |
| S710 | Phosphorylation | Uniprot | |
| K718 | Acetylation | Uniprot | |
| S742 | Phosphorylation | Uniprot | |
| K782 | Ubiquitination | Uniprot | |
| K857 | Ubiquitination | Uniprot | |
| T887 | Phosphorylation | Uniprot | |
| T890 | Phosphorylation | Uniprot | |
| T897 | Phosphorylation | Uniprot | |
| K898 | Ubiquitination | Uniprot | |
| Y913 | Phosphorylation | Uniprot | |
| T917 | Phosphorylation | Uniprot | |
| K921 | Ubiquitination | Uniprot | |
| K950 | Ubiquitination | Uniprot | |
| T1024 | Phosphorylation | P41743 (PRKCI) | Uniprot |
| K1057 | Ubiquitination | Uniprot | |
| K1093 | Ubiquitination | Uniprot | |
| S1098 | Phosphorylation | Uniprot | |
| S1100 | Phosphorylation | Uniprot | |
| T1101 | Phosphorylation | Uniprot | |
| S1102 | Phosphorylation | Uniprot | |
| S1105 | Phosphorylation | Uniprot | |
| S1108 | Phosphorylation | Uniprot | |
| T1112 | Phosphorylation | Uniprot | |
| S1119 | Phosphorylation | Uniprot | |
| Y1153 | Phosphorylation | Uniprot | |
| T1180 | Phosphorylation | Uniprot | |
| T1189 | Phosphorylation | Uniprot | |
| K1194 | Acetylation | Uniprot | |
| Y1287 | Phosphorylation | Uniprot | |
| S1322 | Phosphorylation | Uniprot | |
| S1328 | Phosphorylation | Uniprot | |
| T1331 | Phosphorylation | Uniprot | |
| S1333 | Phosphorylation | P41743 (PRKCI) | Uniprot |
| T1334 | Phosphorylation | P41743 (PRKCI) | Uniprot |
| S1336 | Phosphorylation | Uniprot | |
| T1337 | Phosphorylation | P41743 (PRKCI) | Uniprot |
| S1341 | Phosphorylation | P41743 (PRKCI) | Uniprot |
翻译修饰 - Q13464 作为激酶
| Substrate | Site | Source |
|---|---|---|
| O00429 (DNM1L) | S637 | Uniprot |
| O14649 (KCNK3) | S336 | Uniprot |
| O14649 (KCNK3) | S393 | Uniprot |
| O14950 (MYL12B) | T19 | Uniprot |
| O14950 (MYL12B) | S20 | Uniprot |
| O14974 (PPP1R12A) | T696 | Uniprot |
| O14974 (PPP1R12A) | S852 | Uniprot |
| O14974 (PPP1R12A) | T853 | Uniprot |
| O43293 (DAPK3) | T265 | Uniprot |
| O43293 (DAPK3) | T299 | Uniprot |
| O60237 (PPP1R12B) | T646 | Uniprot |
| P04637 (TP53) | S15 | Uniprot |
| P07737 (PFN1) | S138 | Uniprot |
| P08670 (VIM) | S72 | Uniprot |
| P14136-1 (GFAP) | T7 | Uniprot |
| P14136 (GFAP) | S13 | Uniprot |
| P14136-1 (GFAP) | S17 | Uniprot |
| P14136 (GFAP) | S38 | Uniprot |
| P15311 (EZR) | T567 | Uniprot |
| P17661 (DES) | S12 | Uniprot |
| P17661 (DES) | T17 | Uniprot |
| P17661 (DES) | S60 | Uniprot |
| P17661 (DES) | T76 | Uniprot |
| P17661 (DES) | T77 | Uniprot |
| P19105 (MYL12A) | T18 | Uniprot |
| P19105 (MYL12A) | S19 | Uniprot |
| P19634 (SLC9A1) | T653 | Uniprot |
| P24844 (MYL9) | T19 | Uniprot |
| P24844 (MYL9) | S20 | Uniprot |
| P26038 (MSN) | T558 | Uniprot |
| P29966 (MARCKS) | S159 | Uniprot |
| P35241-1 (RDX) | T564 | Uniprot |
| P35241-1 (RDX) | T573 | Uniprot |
| P35611-1 (ADD1) | T445 | Uniprot |
| P35611-1 (ADD1) | T480 | Uniprot |
| P35611-3 (ADD1) | T511 | Uniprot |
| P48436 (SOX9) | S181 | Uniprot |
| P49815 (TSC2) | T1203 | Uniprot |
| P53667 (LIMK1) | T508 | Uniprot |
| P53671 (LIMK2) | T505 | Uniprot |
| P53671-1 (LIMK2) | T526 | Uniprot |
| P60484 (PTEN) | S229 | Uniprot |
| P60484 (PTEN) | T232 | Uniprot |
| P61587 (RND3) | S7 | Uniprot |
| P61587 (RND3) | S11 | Uniprot |
| P61587 (RND3) | S218 | Uniprot |
| P61587 (RND3) | S240 | Uniprot |
| Q05397 (PTK2) | S732 | Uniprot |
| Q14160 (SCRIB) | S1378 | Uniprot |
| Q14160 (SCRIB) | S1508 | Uniprot |
| Q16555-1 (DPYSL2) | T555 | Uniprot |
| Q8N264 (ARHGAP24) | S391 | Uniprot |
| Q8N264 (ARHGAP24) | S402 | Uniprot |
| Q8N264 (ARHGAP24) | S413 | Uniprot |
| Q8N264 (ARHGAP24) | S415 | Uniprot |
| Q8N264-1 (ARHGAP24) | S437 | Uniprot |
| Q8N264 (ARHGAP24) | T452 | Uniprot |
| Q8N264 (ARHGAP24) | S573 | Uniprot |
| Q8N264 (ARHGAP24) | S574 | Uniprot |
| Q8N264-1 (ARHGAP24) | T575 | Uniprot |
| Q8N264 (ARHGAP24) | T576 | Uniprot |
| Q8N264 (ARHGAP24) | T577 | Uniprot |
| Q9NRY4 (ARHGAP35) | S1150 | Uniprot |
| Q9NRY4 (ARHGAP35) | T1173 | Uniprot |
| Q9NRY4 (ARHGAP35) | S1174 | Uniprot |
| Q9NRY4 (ARHGAP35) | T1226 | Uniprot |
| Q9NRY4 (ARHGAP35) | S1236 | Uniprot |
| Q9UPT6 (MAPK8IP3) | S314 | Uniprot |
| Q9UPT6 (MAPK8IP3) | S364 | Uniprot |
| Q9UPT6 (MAPK8IP3) | S365 | Uniprot |
| Q9Y613 (FHOD1) | S1131 | Uniprot |
| Q9Y613 (FHOD1) | S1137 | Uniprot |
| Q9Y613 (FHOD1) | T1141 | Uniprot |
研究背景
Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of DAPK3, GFAP, LIMK1, LIMK2, MYL9/MLC2, TPPP, PFN1 and PPP1R12A. Phosphorylates FHOD1 and acts synergistically with it to promote SRC-dependent non-apoptotic plasma membrane blebbing. Phosphorylates JIP3 and regulates the recruitment of JNK to JIP3 upon UVB-induced stress. Acts as a suppressor of inflammatory cell migration by regulating PTEN phosphorylation and stability (By similarity). Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Required for centrosome positioning and centrosome-dependent exit from mitosis (By similarity). Plays a role in terminal erythroid differentiation. May regulate closure of the eyelids and ventral body wall by inducing the assembly of actomyosin bundles (By similarity). Promotes keratinocyte terminal differentiation. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization (By similarity).
Autophosphorylated on serine and threonine residues.
Cleaved by caspase-3 during apoptosis. This leads to constitutive activation of the kinase and membrane blebbing.
Cytoplasm. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome>Centriole. Golgi apparatus membrane>Peripheral membrane protein. Cell projection>Bleb. Cytoplasm>Cytoskeleton. Cell membrane. Cell projection>Lamellipodium. Cell projection>Ruffle.
Note: Associated with the mother centriole and an intercentriolar linker. Colocalizes with ITGB1BP1 and ITGB1 at the cell membrane predominantly in lamellipodia and membrane ruffles, but also in retraction fibers. Localizes at the cell membrane in an ITGB1BP1-dependent manner (By similarity). A small proportion is associated with Golgi membranes.
Detected in blood platelets.
Homodimer. Interacts with RHOB, RHOC, MYLC2B and PTEN. Interacts with ITGB1BP1 (via N-terminus and PTB domain) (By similarity). Interacts with RHOA (activated by GTP), CHORDC1, DAPK3, GEM, JIP3, RHOE, PPP1R12A, PFN1, LIMK1, LIMK2 and TSG101. Interacts with FHOD1 in a Src-dependent manner. Interacts with SHROOM3.
The C-terminal auto-inhibitory domain interferes with kinase activity. RHOA binding leads to a conformation change and activation of the kinase. Truncated ROCK1 is constitutively activated.
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
研究领域
· Cellular Processes > Cellular community - eukaryotes > Focal adhesion. (View pathway)
· Cellular Processes > Cellular community - eukaryotes > Tight junction. (View pathway)
· Cellular Processes > Cell motility > Regulation of actin cytoskeleton. (View pathway)
· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > cAMP signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Sphingolipid signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > TGF-beta signaling pathway. (View pathway)
· Human Diseases > Infectious diseases: Bacterial > Pathogenic Escherichia coli infection.
· Human Diseases > Infectious diseases: Bacterial > Shigellosis.
· Human Diseases > Infectious diseases: Bacterial > Salmonella infection.
· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)
· Human Diseases > Cancers: Overview > Proteoglycans in cancer.
· Human Diseases > Cancers: Overview > MicroRNAs in cancer.
· Organismal Systems > Immune system > Chemokine signaling pathway. (View pathway)
· Organismal Systems > Circulatory system > Vascular smooth muscle contraction. (View pathway)
· Organismal Systems > Development > Axon guidance. (View pathway)
· Organismal Systems > Immune system > Platelet activation. (View pathway)
· Organismal Systems > Immune system > Leukocyte transendothelial migration. (View pathway)
· Organismal Systems > Endocrine system > Oxytocin signaling pathway.
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