| 产品: | 磷酸化 KIBRA (Ser947) 抗体 |
| 货号: | AF4317 |
| 描述: | Rabbit polyclonal antibody to Phospho-KIBRA (Ser947) |
| 应用: | WB |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 135kDa; 125kD(Calculated). |
| 蛋白号: | Q8IX03 |
| RRID: | AB_2844396 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF4317, RRID:AB_2844396.
展开/折叠
FLJ10865; FLJ23369; HBeAg-binding protein 3; HBEBP3; HBEBP36; KIAA0869; KIBRA; Kidney and brain protein; Protein KIBRA; Protein WWC1; WW and C2 domain containing 1; WW C2 and coiled-coil domain containing 1; WW domain-containing protein 1; WWC1;
抗原和靶标
Expressed in mammary epithelial cells and breast cancer cell lines. Found in the luminal epithelium surrounding the ducts in the normal breast. In the brain, expressed in somatodendritic compartment of neurons in the cortex and hippocampus and in the cerebellum it is found in the Purkinje cells and some granule cells (at protein level). Detected in brain, heart, colon and kidney. In the kidney, expressed in glomerular podocytes, in some tubules and in the collecting duct.
- Q8IX03 KIBRA_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MPRPELPLPEGWEEARDFDGKVYYIDHTNRTTSWIDPRDRYTKPLTFADCISDELPLGWEEAYDPQVGDYFIDHNTKTTQIEDPRVQWRREQEHMLKDYLVVAQEALSAQKEIYQVKQQRLELAQQEYQQLHAVWEHKLGSQVSLVSGSSSSSKYDPEILKAEIATAKSRVNKLKREMVHLQHELQFKERGFQTLKKIDKKMSDAQGSYKLDEAQAVLRETKAIKKAITCGEKEKQDLIKSLAMLKDGFRTDRGSHSDLWSSSSSLESSSFPLPKQYLDVSSQTDISGSFGINSNNQLAEKVRLRLRYEEAKRRIANLKIQLAKLDSEAWPGVLDSERDRLILINEKEELLKEMRFISPRKWTQGEVEQLEMARKRLEKDLQAARDTQSKALTERLKLNSKRNQLVRELEEATRQVATLHSQLKSLSSSMQSLSSGSSPGSLTSSRGSLVASSLDSSTSASFTDLYYDPFEQLDSELQSKVEFLLLEGATGFRPSGCITTIHEDEVAKTQKAEGGGRLQALRSLSGTPKSMTSLSPRSSLSSPSPPCSPLMADPLLAGDAFLNSLEFEDPELSATLCELSLGNSAQERYRLEEPGTEGKQLGQAVNTAQGCGLKVACVSAAVSDESVAGDSGVYEASVQRLGASEAAAFDSDESEAVGATRIQIALKYDEKNKQFAILIIQLSNLSALLQQQDQKVNIRVAVLPCSESTTCLFRTRPLDASDTLVFNEVFWVSMSYPALHQKTLRVDVCTTDRSHLEECLGGAQISLAEVCRSGERSTRWYNLLSYKYLKKQSRELKPVGVMAPASGPASTDAVSALLEQTAVELEKRQEGRSSTQTLEDSWRYEETSENEAVAEEEEEEVEEEEGEEDVFTEKASPDMDGYPALKVDKETNTETPAPSPTVVRPKDRRVGTPSQGPFLRGSTIIRSKTFSPGPQSQYVCRLNRSDSDSSTLSKKPPFVRNSLERRSVRMKRPSSVKSLRSERLIRTSLDLELDLQATRTWHSQLTQEISVLKELKEQLEQAKSHGEKELPQWLREDERFRLLLRMLEKRQMDRAEHKGELQTDKMMRAAAKDVHRLRGQSCKEPPEVQSFREKMAFFTRPRMNIPALSADDV
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q8IX03 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K117 | Ubiquitination | Uniprot | |
| Y128 | Phosphorylation | Uniprot | |
| S141 | Phosphorylation | Uniprot | |
| K154 | Acetylation | Uniprot | |
| K161 | Ubiquitination | Uniprot | |
| S208 | Phosphorylation | Uniprot | |
| S241 | Phosphorylation | Uniprot | |
| S255 | Phosphorylation | Uniprot | |
| K324 | Ubiquitination | Uniprot | |
| K347 | Ubiquitination | Uniprot | |
| K379 | Ubiquitination | Uniprot | |
| S427 | Phosphorylation | Uniprot | |
| S438 | Phosphorylation | Uniprot | |
| S523 | Phosphorylation | Uniprot | |
| S525 | Phosphorylation | Uniprot | |
| T527 | Phosphorylation | Uniprot | |
| S530 | Phosphorylation | Uniprot | |
| T532 | Phosphorylation | Uniprot | |
| S533 | Phosphorylation | Uniprot | |
| S535 | Phosphorylation | Uniprot | |
| S539 | Phosphorylation | Q96GD4 (AURKB) , O14965 (AURKA) | Uniprot |
| S542 | Phosphorylation | P28482 (MAPK1) , P27361 (MAPK3) , P06493 (CDK1) | Uniprot |
| S548 | Phosphorylation | P28482 (MAPK1) , P27361 (MAPK3) | Uniprot |
| S623 | Phosphorylation | Uniprot | |
| S626 | Phosphorylation | Uniprot | |
| S651 | Phosphorylation | Uniprot | |
| Y781 | Phosphorylation | Uniprot | |
| Y786 | Phosphorylation | Uniprot | |
| Y788 | Phosphorylation | Uniprot | |
| S815 | Phosphorylation | Uniprot | |
| S834 | Phosphorylation | Uniprot | |
| T837 | Phosphorylation | Uniprot | |
| S841 | Phosphorylation | Uniprot | |
| S876 | Phosphorylation | Uniprot | |
| T893 | Phosphorylation | Uniprot | |
| T895 | Phosphorylation | Uniprot | |
| S899 | Phosphorylation | Uniprot | |
| T901 | Phosphorylation | Uniprot | |
| T912 | Phosphorylation | Uniprot | |
| S922 | Phosphorylation | Uniprot | |
| T923 | Phosphorylation | Uniprot | |
| S927 | Phosphorylation | Uniprot | |
| T929 | Phosphorylation | Q15418 (RPS6KA1) , P51812 (RPS6KA3) | Uniprot |
| S931 | Phosphorylation | P06493 (CDK1) | Uniprot |
| Y938 | Phosphorylation | Uniprot | |
| S945 | Phosphorylation | Uniprot | |
| S947 | Phosphorylation | Q15418 (RPS6KA1) , P51812 (RPS6KA3) | Uniprot |
| S962 | Phosphorylation | Uniprot | |
| S975 | Phosphorylation | Q05513 (PRKCZ) | Uniprot |
| S978 | Phosphorylation | Q05513 (PRKCZ) | Uniprot |
| T1006 | Phosphorylation | Uniprot | |
| S1081 | Phosphorylation | Uniprot |
研究背景
Probable regulator of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway, a signaling pathway that plays a pivotal role in tumor suppression by restricting proliferation and promoting apoptosis. Along with NF2 can synergistically induce the phosphorylation of LATS1 and LATS2 and can probably function in the regulation of the Hippo/SWH (Sav/Wts/Hpo) signaling pathway. Acts as a transcriptional coactivator of ESR1 which plays an essential role in DYNLL1-mediated ESR1 transactivation. Regulates collagen-stimulated activation of the ERK/MAPK cascade. Modulates directional migration of podocytes. Acts as a substrate for PRKCZ. Plays a role in cognition and memory performance.
Phosphorylation at Ser-542 and Ser-931 by CDK1 in response to spindle damage stress regulates mitotic exit, these two sites are dephosphorylated by CDC14B.
Cytoplasm. Cytoplasm>Perinuclear region. Nucleus. Cell projection>Ruffle membrane.
Note: Colocalizes with PRKCZ in the perinuclear region.
Expressed in mammary epithelial cells and breast cancer cell lines. Found in the luminal epithelium surrounding the ducts in the normal breast. In the brain, expressed in somatodendritic compartment of neurons in the cortex and hippocampus and in the cerebellum it is found in the Purkinje cells and some granule cells (at protein level). Detected in brain, heart, colon and kidney. In the kidney, expressed in glomerular podocytes, in some tubules and in the collecting duct.
Homodimer. Interacts with DDN. Interacts with DYNLL1 and histone H3. The interaction with DYNLL1 is mandatory for the recruitment and transactivation functions of ESR1 or DYNLL1 to the target chromatin and the interaction with histone H3 ensures proper regulatory interaction of WWC1-DYNLL1-ESR1 complexes with target chromatin. Interacts (via WW domains) with DDR1 (via PPxY motif) in a collagen-regulated manner. Interacts with PRKCZ (via the protein kinase domain). Forms a tripartite complex with DDR1 and PRKCZ, but predominantly in the absence of collagen. Interacts (via the ADDV motif) with PATJ (via PDZ domain 8). Interacts (via WW domains) with SYNPO (via PPxY motifs). Interacts with NF2 and SNX4.
The C2-domain mediates homodimerization. It is a calcium-sensitive lipid-binding domain with preference for PI(3)P.
Belongs to the WWC family. KIBRA subfamily.
研究领域
· Environmental Information Processing > Signal transduction > Hippo signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Hippo signaling pathway - multiple species. (View pathway)
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