| 产品: | 磷酸化 HPK1 (Ser421) 抗体 |
| 货号: | AF4328 |
| 描述: | Rabbit polyclonal antibody to Phospho-HPK1 (Ser421) |
| 应用: | WB |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Sheep, Rabbit, Dog |
| 分子量: | 97kDa; 91kD(Calculated). |
| 蛋白号: | Q92918 |
| RRID: | AB_2844407 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF4328, RRID:AB_2844407.
展开/折叠
Hematopoietic progenitor kinase 1; Hematopoietic progenitor kinase; HPK1; Human Hematopoietic Progenitor Kinase; M4K1_HUMAN; Map4k1; MAPK/ERK kinase kinase kinase 1; MEK kinase kinase 1; MEKKK 1; Mitogen activated protein kinase kinase kinase kinase 1; Mitogen-activated protein kinase kinase kinase kinase 1;
抗原和靶标
Expressed primarily in hematopoietic organs, including bone marrow, spleen and thymus. Also expressed at very low levels in lung, kidney, mammary glands and small intestine.
- Q92918 M4K1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDVVDPDIFNRDPRDHYDLLQRLGGGTYGEVFKARDKVSGDLVALKMVKMEPDDDVSTLQKEILILKTCRHANIVAYHGSYLWLQKLWICMEFCGAGSLQDIYQVTGSLSELQISYVCREVLQGLAYLHSQKKIHRDIKGANILINDAGEVRLADFGISAQIGATLARRLSFIGTPYWMAPEVAAVALKGGYNELCDIWSLGITAIELAELQPPLFDVHPLRVLFLMTKSGYQPPRLKEKGKWSAAFHNFIKVTLTKSPKKRPSATKMLSHQLVSQPGLNRGLILDLLDKLKNPGKGPSIGDIEDEEPELPPAIPRRIRSTHRSSSLGIPDADCCRRHMEFRKLRGMETRPPANTARLQPPRDLRSSSPRKQLSESSDDDYDDVDIPTPAEDTPPPLPPKPKFRSPSDEGPGSMGDDGQLSPGVLVRCASGPPPNSPRPGPPPSTSSPHLTAHSEPSLWNPPSRELDKPPLLPPKKEKMKRKGCALLVKLFNGCPLRIHSTAAWTHPSTKDQHLLLGAEEGIFILNRNDQEATLEMLFPSRTTWVYSINNVLMSLSGKTPHLYSHSILGLLERKETRAGNPIAHISPHRLLARKNMVSTKIQDTKGCRACCVAEGASSGGPFLCGALETSVVLLQWYQPMNKFLLVRQVLFPLPTPLSVFALLTGPGSELPAVCIGVSPGRPGKSVLFHTVRFGALSCWLGEMSTEHRGPVQVTQVEEDMVMVLMDGSVKLVTPEGSPVRGLRTPEIPMTEAVEAVAMVGGQLQAFWKHGVQVWALGSDQLLQELRDPTLTFRLLGSPRLECSGTISPHCNLLLPGSSNSPASASRVAGITGL
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q92918 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y28 | Phosphorylation | Uniprot | |
| K33 | Ubiquitination | Uniprot | |
| K37 | Ubiquitination | Uniprot | |
| K46 | Ubiquitination | Uniprot | |
| K49 | Ubiquitination | Uniprot | |
| T165 | Phosphorylation | Q92918 (MAP4K1) | Uniprot |
| S171 | Phosphorylation | Q92918 (MAP4K1) , Q15139 (PRKD1) | Uniprot |
| T175 | Phosphorylation | Q92918 (MAP4K1) | Uniprot |
| Y177 | Phosphorylation | Uniprot | |
| S230 | Phosphorylation | Uniprot | |
| Y232 | Phosphorylation | P00519 (ABL1) | Uniprot |
| S258 | Phosphorylation | Uniprot | |
| K296 | Ubiquitination | Uniprot | |
| S320 | Phosphorylation | Uniprot | |
| S324 | Phosphorylation | Uniprot | |
| S325 | Phosphorylation | Uniprot | |
| S326 | Phosphorylation | Uniprot | |
| T349 | Phosphorylation | Uniprot | |
| T355 | Phosphorylation | Q92918 (MAP4K1) | Uniprot |
| S366 | Phosphorylation | Uniprot | |
| S368 | Phosphorylation | Uniprot | |
| S374 | Phosphorylation | Uniprot | |
| S376 | Phosphorylation | Uniprot | |
| S377 | Phosphorylation | Uniprot | |
| Y381 | Phosphorylation | P43405 (SYK) | Uniprot |
| S405 | Phosphorylation | Uniprot | |
| S407 | Phosphorylation | Uniprot | |
| S413 | Phosphorylation | Uniprot | |
| S421 | Phosphorylation | Uniprot | |
| S430 | Phosphorylation | Uniprot | |
| S436 | Phosphorylation | Uniprot | |
| S444 | Phosphorylation | Uniprot | |
| S446 | Phosphorylation | Uniprot | |
| S447 | Phosphorylation | Uniprot | |
| T451 | Phosphorylation | Uniprot | |
| S454 | Phosphorylation | Uniprot | |
| S586 | Phosphorylation | Uniprot | |
| K594 | Ubiquitination | Uniprot | |
| S598 | Phosphorylation | Uniprot | |
| T599 | Phosphorylation | Uniprot | |
| K600 | Ubiquitination | Uniprot | |
| S737 | Phosphorylation | Uniprot |
翻译修饰 - Q92918 作为激酶
| Substrate | Site | Source |
|---|---|---|
| Q13094 (LCP2) | S376 | Uniprot |
| Q16584 (MAP3K11) | S281 | Uniprot |
| Q92918 (MAP4K1) | T165 | Uniprot |
| Q92918 (MAP4K1) | S171 | Uniprot |
| Q92918 (MAP4K1) | T175 | Uniprot |
| Q92918 (MAP4K1) | T355 | Uniprot |
| Q92918-2 (MAP4K1) | S376 | Uniprot |
| Q92918-2 (MAP4K1) | S377 | Uniprot |
| Q92918-2 (MAP4K1) | Y381 | Uniprot |
| Q92918-2 (MAP4K1) | S407 | Uniprot |
| Q92918-1 (MAP4K1) | S421 | Uniprot |
| Q92918-1 (MAP4K1) | S586 | Uniprot |
| Q9BXL7 (CARD11) | S556 | Uniprot |
| Q9BXL7 (CARD11) | S558 | Uniprot |
研究背景
Serine/threonine-protein kinase, which may play a role in the response to environmental stress. Appears to act upstream of the JUN N-terminal pathway. May play a role in hematopoietic lineage decisions and growth regulation. Able to autophosphorylate. Together with CLNK, it enhances CD3-triggered activation of T-cells and subsequent IL2 production (By similarity).
Autophosphorylates: phosphorylation promotes ubiquitination by the Cul7-RING(FBXW8) ubiquitin-protein ligase complex, leading to its degradation by the proteasome.
Tyrosine-phosphorylated after activation of hemopoietic cells.
Ubiquitinated by the Cul7-RING(FBXW8) ubiquitin-protein ligase complex following autophosphorylation, leading to its degradation by the proteasome.
Expressed primarily in hematopoietic organs, including bone marrow, spleen and thymus. Also expressed at very low levels in lung, kidney, mammary glands and small intestine.
Interacts with MAP3K1. Interacts with FBXW8. Interacts with CLNK (via its SH2 domain) (By similarity).
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
研究领域
· Environmental Information Processing > Signal transduction > MAPK signaling pathway. (View pathway)
限制条款
产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。
产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。
Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。产品仅供科学研究使用。不用于诊断和治疗。
产品未经授权不得转售。
Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.