Phospho-ATP citrate lyase (Ser455/Ser457) Antibody - #AF4368
产品: | 磷酸化 ATP citrate lyase (Ser455/Ser457) 抗体 |
货号: | AF4368 |
描述: | Rabbit polyclonal antibody to Phospho-ATP citrate lyase (Ser455/Ser457) |
应用: | WB |
反应: | Human, Mouse, Rat, Monkey |
预测: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
分子量: | 125kDa; 121kD(Calculated). |
蛋白号: | P53396 |
RRID: | AB_2844433 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF4368, RRID:AB_2844433.
展开/折叠
ACL; Acly; ACLY_HUMAN; ATP citrate (pro-S) lyase; ATP citrate lyase; ATP citrate synthase; ATP-citrate (pro-S-)-lyase; ATP-citrate synthase; ATPcitrate synthase; ATPCL; Citrate cleavage enzyme; CLATP; OTTHUMP00000164773;
抗原和靶标
- P53396 ACLY_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSAKAISEQTGKELLYKFICTTSAIQNRFKYARVTPDTDWARLLQDHPWLLSQNLVVKPDQLIKRRGKLGLVGVNLTLDGVKSWLKPRLGQEATVGKATGFLKNFLIEPFVPHSQAEEFYVCIYATREGDYVLFHHEGGVDVGDVDAKAQKLLVGVDEKLNPEDIKKHLLVHAPEDKKEILASFISGLFNFYEDLYFTYLEINPLVVTKDGVYVLDLAAKVDATADYICKVKWGDIEFPPPFGREAYPEEAYIADLDAKSGASLKLTLLNPKGRIWTMVAGGGASVVYSDTICDLGGVNELANYGEYSGAPSEQQTYDYAKTILSLMTREKHPDGKILIIGGSIANFTNVAATFKGIVRAIRDYQGPLKEHEVTIFVRRGGPNYQEGLRVMGEVGKTTGIPIHVFGTETHMTAIVGMALGHRPIPNQPPTAAHTANFLLNASGSTSTPAPSRTASFSESRADEVAPAKKAKPAMPQDSVPSPRSLQGKSTTLFSRHTKAIVWGMQTRAVQGMLDFDYVCSRDEPSVAAMVYPFTGDHKQKFYWGHKEILIPVFKNMADAMRKHPEVDVLINFASLRSAYDSTMETMNYAQIRTIAIIAEGIPEALTRKLIKKADQKGVTIIGPATVGGIKPGCFKIGNTGGMLDNILASKLYRPGSVAYVSRSGGMSNELNNIISRTTDGVYEGVAIGGDRYPGSTFMDHVLRYQDTPGVKMIVVLGEIGGTEEYKICRGIKEGRLTKPIVCWCIGTCATMFSSEVQFGHAGACANQASETAVAKNQALKEAGVFVPRSFDELGEIIQSVYEDLVANGVIVPAQEVPPPTVPMDYSWARELGLIRKPASFMTSICDERGQELIYAGMPITEVFKEEMGIGGVLGLLWFQKRLPKYSCQFIEMCLMVTADHGPAVSGAHNTIICARAGKDLVSSLTSGLLTIGDRFGGALDAAAKMFSKAFDSGIIPMEFVNKMKKEGKLIMGIGHRVKSINNPDMRVQILKDYVRQHFPATPLLDYALEVEKITTSKKPNLILNVDGLIGVAFVDMLRNCGSFTREEADEYIDIGALNGIFVLGRSMGFIGHYLDQKRLKQGLYRHPWDDISYVLPEHMSM
种属预测
score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P53396 作为底物
Site | PTM Type | Enzyme | Source |
---|---|---|---|
K4 | Ubiquitination | Uniprot | |
K12 | Ubiquitination | Uniprot | |
K17 | Ubiquitination | Uniprot | |
C20 | S-Nitrosylation | Uniprot | |
S23 | Phosphorylation | Uniprot | |
T38 | Phosphorylation | Uniprot | |
K58 | Ubiquitination | Uniprot | |
K64 | Acetylation | Uniprot | |
K64 | Ubiquitination | Uniprot | |
K68 | Ubiquitination | Uniprot | |
K82 | Ubiquitination | Uniprot | |
S83 | Phosphorylation | Uniprot | |
K86 | Acetylation | Uniprot | |
K86 | Ubiquitination | Uniprot | |
T94 | Phosphorylation | Uniprot | |
K97 | Ubiquitination | Uniprot | |
T99 | Phosphorylation | Uniprot | |
Y131 | Phosphorylation | Uniprot | |
K148 | Ubiquitination | Uniprot | |
K151 | Acetylation | Uniprot | |
K151 | Ubiquitination | Uniprot | |
K159 | Acetylation | Uniprot | |
K159 | Ubiquitination | Uniprot | |
K166 | Ubiquitination | Uniprot | |
K167 | Ubiquitination | Uniprot | |
Y213 | Phosphorylation | Uniprot | |
C229 | S-Nitrosylation | Uniprot | |
K230 | Ubiquitination | Uniprot | |
K232 | Ubiquitination | Uniprot | |
Y247 | Phosphorylation | Uniprot | |
Y252 | Phosphorylation | Uniprot | |
K259 | Acetylation | Uniprot | |
K259 | Ubiquitination | Uniprot | |
S260 | Phosphorylation | Uniprot | |
S263 | Phosphorylation | Uniprot | |
K265 | Ubiquitination | Uniprot | |
K272 | Ubiquitination | Uniprot | |
Y317 | Phosphorylation | Uniprot | |
T322 | Phosphorylation | Uniprot | |
S325 | Phosphorylation | Uniprot | |
Y364 | Phosphorylation | Uniprot | |
K369 | Acetylation | Uniprot | |
K369 | Ubiquitination | Uniprot | |
R379 | Methylation | Uniprot | |
Y384 | Phosphorylation | Uniprot | |
R389 | Methylation | Uniprot | |
S442 | Phosphorylation | Uniprot | |
S444 | Phosphorylation | Uniprot | |
T445 | Phosphorylation | Uniprot | |
S446 | Phosphorylation | Uniprot | |
T447 | Phosphorylation | Uniprot | |
S451 | Phosphorylation | Uniprot | |
T453 | Phosphorylation | Uniprot | |
S455 | Phosphorylation | Uniprot | |
S457 | Phosphorylation | Uniprot | |
S459 | Phosphorylation | Uniprot | |
K468 | Ubiquitination | Uniprot | |
K471 | Ubiquitination | Uniprot | |
S478 | Phosphorylation | Uniprot | |
S481 | Phosphorylation | Uniprot | |
S484 | Phosphorylation | Uniprot | |
K488 | Ubiquitination | Uniprot | |
K498 | Ubiquitination | Uniprot | |
Y531 | Phosphorylation | Uniprot | |
K538 | Acetylation | Uniprot | |
K538 | Ubiquitination | Uniprot | |
K540 | Acetylation | Uniprot | |
K540 | Ubiquitination | Uniprot | |
K546 | Acetylation | Uniprot | |
K546 | Ubiquitination | Uniprot | |
K554 | Acetylation | Uniprot | |
K554 | Ubiquitination | Uniprot | |
K562 | Ubiquitination | Uniprot | |
S574 | Phosphorylation | Uniprot | |
S581 | Phosphorylation | Uniprot | |
T582 | Phosphorylation | Uniprot | |
Y588 | Phosphorylation | Uniprot | |
T593 | Phosphorylation | Uniprot | |
T606 | Phosphorylation | Uniprot | |
K616 | Ubiquitination | Uniprot | |
K630 | Acetylation | Uniprot | |
K630 | Ubiquitination | Uniprot | |
K635 | Acetylation | Uniprot | |
K635 | Ubiquitination | Uniprot | |
T639 | Phosphorylation | Uniprot | |
S649 | Phosphorylation | Uniprot | |
K650 | Ubiquitination | Uniprot | |
Y652 | Phosphorylation | Uniprot | |
S656 | Phosphorylation | Uniprot | |
Y659 | Phosphorylation | Uniprot | |
S663 | Phosphorylation | Uniprot | |
S667 | Phosphorylation | Uniprot | |
S675 | Phosphorylation | Uniprot | |
T677 | Phosphorylation | Uniprot | |
Y682 | Phosphorylation | Uniprot | |
R691 | Methylation | Uniprot | |
Y692 | Phosphorylation | Uniprot | |
T707 | Phosphorylation | Uniprot | |
H760 | Phosphorylation | Uniprot | |
K780 | Ubiquitination | Uniprot | |
K836 | Ubiquitination | Uniprot | |
S839 | Phosphorylation | Uniprot | |
Y854 | Phosphorylation | Uniprot | |
K918 | Ubiquitination | Uniprot | |
T930 | Phosphorylation | Uniprot | |
K944 | Ubiquitination | Uniprot | |
K948 | Acetylation | Uniprot | |
K948 | Ubiquitination | Uniprot | |
S952 | Phosphorylation | Uniprot | |
K962 | Acetylation | Uniprot | |
K962 | Ubiquitination | Uniprot | |
K964 | Methylation | Uniprot | |
K965 | Methylation | Uniprot | |
K968 | Acetylation | Uniprot | |
K968 | Ubiquitination | Uniprot | |
K978 | Acetylation | Uniprot | |
K978 | Ubiquitination | Uniprot | |
S979 | Phosphorylation | Uniprot | |
R986 | Methylation | Uniprot | |
K991 | Ubiquitination | Uniprot | |
T1001 | Phosphorylation | Uniprot | |
K1012 | Ubiquitination | Uniprot | |
Y1073 | Phosphorylation | Uniprot | |
K1077 | Acetylation | Uniprot | |
K1077 | Ubiquitination | Uniprot | |
K1080 | Ubiquitination | Uniprot | |
Y1084 | Phosphorylation | Uniprot | |
S1100 | Phosphorylation | Uniprot |
研究背景
Catalyzes the cleavage of citrate into oxaloacetate and acetyl-CoA, the latter serving as common substrate for de novo cholesterol and fatty acid synthesis.
Phosphorylated by PKA and GSK3 in a sequential manner; phosphorylation results in activation of its activity. Phosphorylation on Thr-447 and Ser-451 depends on the phosphorylation state of Ser-455 (By similarity). Phosphorylation on Ser-455 is decreased by prior phosphorylation on the other 2 residues (By similarity).
ISGylated.
Acetylated at Lys-540, Lys-546 and Lys-554 by KAT2B/PCAF. Acetylation is promoted by glucose and stabilizes the protein, probably by preventing ubiquitination at the same sites. Acetylation promotes de novo lipid synthesis. Deacetylated by SIRT2.
Ubiquitinated at Lys-540, Lys-546 and Lys-554 by UBR4, leading to its degradation. Ubiquitination is probably inhibited by acetylation at same site (Probable).
Cytoplasm>Cytosol.
Homotetramer.
In the N-terminal section; belongs to the succinate/malate CoA ligase beta subunit family.
In the C-terminal section; belongs to the succinate/malate CoA ligase alpha subunit family.
研究领域
· Metabolism > Carbohydrate metabolism > Citrate cycle (TCA cycle).
· Metabolism > Global and overview maps > Metabolic pathways.
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