产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF4381, RRID:AB_2844446.
展开/折叠
Alpha endosulfine; ARPP 19e; Endosulfine alpha;
抗原和靶标
Widely expressed with high levels in skeletal muscle and brain and lower levels in the pancreas.
- O43768 ENSA_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSQKQEEENPAEETGEEKQDTQEKEGILPERAEEAKLKAKYPSLGQKPGGSDFLMKRLQKGQKYFDSGDYNMAKAKMKNKQLPSAGPDKNLVTGDHIPTPQDLPQRKSSLVTSKLAGGQVE
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - O43768 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Acetylation | Uniprot | |
| S2 | Phosphorylation | Uniprot | |
| T14 | Phosphorylation | Uniprot | |
| T21 | Phosphorylation | Uniprot | |
| Y41 | Phosphorylation | Uniprot | |
| S43 | Phosphorylation | Uniprot | |
| K47 | Acetylation | Uniprot | |
| K47 | Ubiquitination | Uniprot | |
| S51 | Phosphorylation | Uniprot | |
| K56 | Methylation | Uniprot | |
| K56 | Ubiquitination | Uniprot | |
| K63 | Acetylation | Uniprot | |
| K63 | Ubiquitination | Uniprot | |
| Y64 | Phosphorylation | Uniprot | |
| S67 | Phosphorylation | Uniprot | |
| Y70 | Phosphorylation | Uniprot | |
| K74 | Ubiquitination | Uniprot | |
| K76 | Ubiquitination | Uniprot | |
| K80 | Ubiquitination | Uniprot | |
| S84 | Phosphorylation | Uniprot | |
| K89 | Acetylation | Uniprot | |
| K89 | Ubiquitination | Uniprot | |
| T93 | O-Glycosylation | Uniprot | |
| T99 | O-Glycosylation | Uniprot | |
| T99 | Phosphorylation | Uniprot | |
| K107 | Ubiquitination | Uniprot | |
| S108 | Phosphorylation | Uniprot | |
| S109 | Phosphorylation | Uniprot | |
| T112 | Phosphorylation | Uniprot | |
| S113 | Phosphorylation | Uniprot | |
| K114 | Ubiquitination | Uniprot |
研究背景
Protein phosphatase inhibitor that specifically inhibits protein phosphatase 2A (PP2A) during mitosis. When phosphorylated at Ser-67 during mitosis, specifically interacts with PPP2R2D (PR55-delta) and inhibits its activity, leading to inactivation of PP2A, an essential condition to keep cyclin-B1-CDK1 activity high during M phase (By similarity). Also acts as a stimulator of insulin secretion by interacting with sulfonylurea receptor (ABCC8), thereby preventing sulfonylurea from binding to its receptor and reducing K(ATP) channel currents.
Phosphorylation at Ser-67 by GWL during mitosis is essential for interaction with PPP2R2D (PR55-delta) and subsequent inactivation of PP2A (By similarity). Phosphorylated by PKA.
Cytoplasm.
Widely expressed with high levels in skeletal muscle and brain and lower levels in the pancreas.
Interacts (when phosphorylated at Ser-67) with PPP2R2D (By similarity). Interacts with ABCC8. Interacts with SNCA; interaction is disrupted when phosphorylated at Ser-109.
Belongs to the endosulfine family.
限制条款
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