Phospho-DOK2 (Tyr351)[Tyr345] Antibody - #AF4383

>>买2送1 进行中>>
>>满百送 进行中>>
>>赠送JD卡 进行中>>
>>3倍积分 进行中>>
(1)  
产品: 磷酸化 DOK2 (Tyr351)[Tyr345] 抗体
货号: AF4383
描述: Rabbit polyclonal antibody to Phospho-DOK2 (Tyr351)[Tyr345]
应用: WB
反应: Human, Mouse
预测: Pig, Zebrafish, Bovine, Horse, Dog
分子量: 56kDa; 45kD(Calculated).
蛋白号: O60496
RRID: AB_2844448

浏览相似产品>>

   规格 价格 库存
 100ul RMB¥ 2800 现货
 200ul RMB¥ 3800 现货

货期: 当天发货

联系销售
相关下载
MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse
预测:
Pig(100%), Zebrafish(82%), Bovine(92%), Horse(92%), Dog(92%)
克隆:
Polyclonal
特异性:
Phospho-DOK2 (Tyr351) Antibody detects endogenous levels of DOK2 only when phosphorylated at Tyr345, which site historically referenced as Tyr351.
RRID:
AB_2844448
引用格式: Affinity Biosciences Cat# AF4383, RRID:AB_2844448.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Docking protein 2 56kDa; Docking protein 2; DOK 2; DOK R; DOK2; DOK2_HUMAN; Downstream of tyrosine kinase 2; OTTHUMP00000161710; OTTHUMP00000224923; p56(dok 2); p56(dok-2); p56dok 2; p56DOK; p56dok2;

抗原和靶标

免疫原:
Uniprot:

O60496(DOK2_HUMAN) >>Visit HPA database.

基因/基因ID:
DOK2(9046)
表达:
O60496 DOK2_HUMAN:

Highly expressed in peripheral blood leukocytes, lymph nodes and spleen. Lower expression in thymus, bone marrow and fetal liver.

序列:
MGDGAVKQGFLYLQQQQTFGKKWRRFGASLYGGSDCALARLELQEGPEKPRRCEAARKVIRLSDCLRVAEAGGEASSPRDTSAFFLETKERLYLLAAPAAERGDWVQAICLLAFPGQRKELSGPEGKQSRPCMEENELYSSAVTVGPHKEFAVTMRPTEASERCHLRGSYTLRAGESALELWGGPEPGTQLYDWPYRFLRRFGRDKVTFSFEAGRRCVSGEGNFEFETRQGNEIFLALEEAISAQKNAAPATPQPQPATIPASLPRPDSPYSRPHDSLPPPSPTTPVPAPRPRGQEGEYAVPFDAVARSLGKNFRGILAVPPQLLADPLYDSIEETLPPRPDHIYDEPEGVAALSLYDSPQEPRGEAWRRQATADRDPAGLQHVQPAGQDFSASGWQPGTEYDNVVLKKGPK

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
92
Bovine
92
Dog
92
Zebrafish
82
Xenopus
50
Sheep
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - O60496 作为底物

Site PTM Type Enzyme
K7 Ubiquitination
Y12 Phosphorylation
S34 Phosphorylation
K49 Ubiquitination
S63 Phosphorylation
S77 Phosphorylation
S122 Phosphorylation
S129 Phosphorylation
Y139 Phosphorylation
S140 Phosphorylation
S141 Phosphorylation
T144 Phosphorylation
S169 Phosphorylation
Y170 Phosphorylation
T189 Phosphorylation
Y192 Phosphorylation
S269 Phosphorylation
Y271 Phosphorylation P08631 (HCK) , P07948 (LYN) , P12931 (SRC)
S277 Phosphorylation
S282 Phosphorylation
Y299 Phosphorylation P08631 (HCK) , P07948 (LYN) , P12931 (SRC)
S309 Phosphorylation
K312 Ubiquitination
Y330 Phosphorylation
S332 Phosphorylation
T336 Phosphorylation
Y345 Phosphorylation P07948 (LYN) , P08631 (HCK) , P12931 (SRC)
Y357 Phosphorylation
Y402 Phosphorylation

研究背景

功能:

DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK2 may modulate the cellular proliferation induced by IL-4, as well as IL-2 and IL-3. May be involved in modulating Bcr-Abl signaling. Attenuates EGF-stimulated MAP kinase activation (By similarity).

翻译修饰:

On immunoreceptor stimulation, phosphorylated on C-terminal tyrosine residues. Phosphorylation on Tyr-345 is required for binding to the SH2 domain of NCK. Phosphorylation on both Tyr-271 and Tyr-299 is required for interaction with RASGAP. Phosphorylated on tyrosine residues by TEK/TIE2 (By similarity).

组织特异性:

Highly expressed in peripheral blood leukocytes, lymph nodes and spleen. Lower expression in thymus, bone marrow and fetal liver.

亚基结构:

Interacts with phosphorylated RASGAP and EGFR. Interacts with RET and NCK. Interacts (via PH domain) with TEK/TIE2 (tyrosine phosphorylated) (By similarity).

(Microbial infection) Interacts with Herpes simplex virus 1 (HHV-1) protein UL46; this interaction induces DOK2 phosphorylation and subsequent degradation.

蛋白家族:

PTB domain mediates receptor interaction.

Belongs to the DOK family. Type A subfamily.

限制条款

产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）

产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。

产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。

Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。

产品仅供科学研究使用。不用于诊断和治疗。 

产品未经授权不得转售。

Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.