产品: 磷酸化 SRF (Ser103) 抗体
货号: AF4403
描述: Rabbit polyclonal antibody to Phospho-SRF (Ser103)
应用: WB IHC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Rabbit, Dog
分子量: 67kDa; 52kD(Calculated).
蛋白号: P11831
RRID: AB_2844468

浏览相似产品>>

   规格 价格 库存
 50ul RMB¥ 1500 现货
 100ul RMB¥ 2800 现货
 200ul RMB¥ 3800 现货

货期: 当天发货

联系销售

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Bovine(100%), Rabbit(89%), Dog(100%)
克隆:
Polyclonal
特异性:
Phospho-SRF (Ser103) Antibody detects endogenous levels of SRF only when phosphorylated at Ser103.
RRID:
AB_2844468
引用格式: Affinity Biosciences Cat# AF4403, RRID:AB_2844468.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

c fos serum response element binding factor; c fos serum response element binding transcription factor; ELK3; ERP; MCM 1; MCM1; OTTHUMP00000039820; SAP2; Serum response factor; SRF; SRF serum response factor c fos serum response element binding transcription factor; SRF_HUMAN;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
序列:
MLPTQAGAAAALGRGSALGGSLNRTPTGRPGGGGGTRGANGGRVPGNGAGLGPGRLEREAAAAAATTPAPTAGALYSGSEGDSESGEEEELGAERRGLKRSLSEMEIGMVVGGPEASAAATGGYGPVSGAVSGAKPGKKTRGRVKIKMEFIDNKLRRYTTFSKRKTGIMKKAYELSTLTGTQVLLLVASETGHVYTFATRKLQPMITSETGKALIQTCLNSPDSPPRSDPTTDQRMSATGFEETDLTYQVSESDSSGETKDTLKPAFTVTNLPGTTSTIQTAPSTSTTMQVSSGPSFPITNYLAPVSASVSPSAVSSANGTVLKSTGSGPVSSGGLMQLPTSFTLMPGGAVAQQVPVQAIQVHQAPQQASPSRDSSTDLTQTSSSGTVTLPATIMTSSVPTTVGGHMMYPSPHAVMYAPTSGLGDGSLTVLNAFSQAPSTMQVSHSQVQEPGGVPQVFLTASSGTVQIPVSAVQLHQMAVIGQQAGSSSNLTELQVVNLDTAHSTKSE

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Pig
100
Bovine
100
Dog
100
Rabbit
89
Horse
0
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P11831 作为底物

Site PTM Type Enzyme
T4 Phosphorylation
R14 Methylation
S16 Phosphorylation
S21 Phosphorylation
R24 Methylation
T27 Phosphorylation
R37 Methylation
T71 Phosphorylation
Y76 Phosphorylation
S77 Phosphorylation P68400 (CSNK2A1)
S79 Phosphorylation P68400 (CSNK2A1)
S83 Phosphorylation P68400 (CSNK2A1)
S85 Phosphorylation P49137 (MAPKAPK2) , P68400 (CSNK2A1)
S101 Phosphorylation
S103 Phosphorylation Q15418 (RPS6KA1) , O75582 (RPS6KA5) , Q9UQM7 (CAMK2A) , P49137 (MAPKAPK2)
K147 Sumoylation
T159 Phosphorylation P17612 (PRKACA) , Q13976 (PRKG1) , Q09013 (DMPK) , P17252 (PRKCA)
T160 Phosphorylation Q9UQM7 (CAMK2A) , Q05655 (PRKCD)
S162 Phosphorylation P17252 (PRKCA)
S176 Phosphorylation
T177 Phosphorylation
T199 Phosphorylation
S221 Phosphorylation
S224 Phosphorylation
S228 Phosphorylation
S251 Phosphorylation
S253 Phosphorylation
S277 O-Glycosylation
S307 O-Glycosylation
S309 O-Glycosylation
S313 O-Glycosylation
S316 O-Glycosylation
S370 Phosphorylation
S383 O-Glycosylation
T401 O-Glycosylation
S435 Phosphorylation P78527 (PRKDC)
S446 Phosphorylation P78527 (PRKDC)

研究背景

功能:

SRF is a transcription factor that binds to the serum response element (SRE), a short sequence of dyad symmetry located 300 bp to the 5' of the site of transcription initiation of some genes (such as FOS). Together with MRTFA transcription coactivator, controls expression of genes regulating the cytoskeleton during development, morphogenesis and cell migration. The SRF-MRTFA complex activity responds to Rho GTPase-induced changes in cellular globular actin (G-actin) concentration, thereby coupling cytoskeletal gene expression to cytoskeletal dynamics. Required for cardiac differentiation and maturation.

翻译修饰:

Phosphorylated by PRKDC.

细胞定位:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Binds DNA as a multimer, probably a dimer. Interacts with MRTFA, forming the SRF-MRTFA nuclear complex which binds the 5'-CArG-3' consensus motif (CArG box) on DNA via SRF. Forms a nuclear ternary complex with MRTFA and SCAI. Interacts with MRTFB. Interacts with MLLT7/FOXO4, NKX3A and SSRP1. Interacts with ARID2 (By similarity). Interacts with SRFBP1 (By similarity). Interacts with FOXK1. Interacts with LPXN. Interacts with OLFM2; the interaction promotes dissociation of SRF from the transcriptional repressor HEY2, facilitates binding of SRF to target genes and promotes smooth muscle differentiation.

研究领域

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

限制条款

产品的规格、报价、验证数据请以官网为准,官网链接:www.affbiotech.com | www.affbiotech.cn(简体中文)| www.affbiotech.jp(日本語)

产品的数据信息为Affinity所有,未经授权不得收集Affinity官网数据或资料用于商业用途,对抄袭产品数据的行为我们将保留诉诸法律的权利。

产品相关数据会因产品批次、产品检测情况随时调整,如您已订购该产品,请以订购时随货说明书为准,否则请以官网内容为准,官网内容有改动时恕不另行通知。

Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时,请您在Affinity官方渠道提交售后申请。

产品仅供科学研究使用。不用于诊断和治疗。 

产品未经授权不得转售。

Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.