产品: 磷酸化 BMX (Tyr40) 抗体
货号: AF4444
描述: Rabbit polyclonal antibody to Phospho-BMX (Tyr40)
应用: WB
反应: Human, Mouse
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 76kDa; 78kD(Calculated).
蛋白号: P51813
RRID: AB_2844508

浏览相似产品>>

   规格 价格 库存
 50ul RMB¥ 1500 现货
 100ul RMB¥ 2800 现货
 200ul RMB¥ 3800 现货

货期: 当天发货

联系销售

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse
预测:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
克隆:
Polyclonal
特异性:
Phospho-BMX (Tyr40) Antibody detects endogenous levels of BMX only when phosphorylated at Tyr40.
RRID:
AB_2844508
引用格式: Affinity Biosciences Cat# AF4444, RRID:AB_2844508.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Bmx; BMX non receptor tyrosine kinase; BMX_HUMAN; BONE MARROW KINASE, X-LINKED; Bone marrow tyrosine kinase gene in chromosome X protein; Cytoplasmic tyrosine protein kinase BMX; Cytoplasmic tyrosine-protein kinase BMX; Epithelial and endothelial tyrosine kinase; ETK; NKT38; NTK 38; NTK38; Protein tyrosine kinase BMX; PSCTK 2; PSCTK 3; PSCTK2; PSCTK3;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
表达:
P51813 BMX_HUMAN:

Highly expressed in cells with great migratory potential, including endothelial cells and metastatic carcinoma cell lines.

序列:
MDTKSILEELLLKRSQQKKKMSPNNYKERLFVLTKTNLSYYEYDKMKRGSRKGSIEIKKIRCVEKVNLEEQTPVERQYPFQIVYKDGLLYVYASNEESRSQWLKALQKEIRGNPHLLVKYHSGFFVDGKFLCCQQSCKAAPGCTLWEAYANLHTAVNEEKHRVPTFPDRVLKIPRAVPVLKMDAPSSSTTLAQYDNESKKNYGSQPPSSSTSLAQYDSNSKKIYGSQPNFNMQYIPREDFPDWWQVRKLKSSSSSEDVASSNQKERNVNHTTSKISWEFPESSSSEEEENLDDYDWFAGNISRSQSEQLLRQKGKEGAFMVRNSSQVGMYTVSLFSKAVNDKKGTVKHYHVHTNAENKLYLAENYCFDSIPKLIHYHQHNSAGMITRLRHPVSTKANKVPDSVSLGNGIWELKREEITLLKELGSGQFGVVQLGKWKGQYDVAVKMIKEGSMSEDEFFQEAQTMMKLSHPKLVKFYGVCSKEYPIYIVTEYISNGCLLNYLRSHGKGLEPSQLLEMCYDVCEGMAFLESHQFIHRDLAARNCLVDRDLCVKVSDFGMTRYVLDDQYVSSVGTKFPVKWSAPEVFHYFKYSSKSDVWAFGILMWEVFSLGKQPYDLYDNSQVVLKVSQGHRLYRPHLASDTIYQIMYSCWHELPEKRPTFQQLLSSIEPLREKDKH

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P51813 作为底物

Site PTM Type Enzyme
Y40 Phosphorylation Q05397 (PTK2)
K172 Acetylation
K181 Acetylation
S186 Phosphorylation
Y216 Phosphorylation Q08881 (ITK) , Q06187 (BTK) , P51813 (BMX) , P42680 (TEC)
Y224 Phosphorylation Q08881 (ITK) , P42680 (TEC) , P51813 (BMX) , Q06187 (BTK)
S226 Phosphorylation
S254 Phosphorylation
S260 Phosphorylation
S261 Phosphorylation
S324 Phosphorylation
Y330 Phosphorylation
K337 Acetylation
K343 Acetylation
K347 Acetylation
Y365 Phosphorylation
T394 Phosphorylation
K395 Acetylation
K413 Ubiquitination
S425 Phosphorylation
Y440 Phosphorylation
Y566 Phosphorylation P12931 (SRC) , P51813 (BMX)
S568 Phosphorylation

翻译修饰 - P51813 作为激酶

Substrate Site Source
P08581 (MET) Y1235 Uniprot
P11362 (FGFR1) Y653 Uniprot
P11362 (FGFR1) Y654 Uniprot
P51813 (BMX) Y216 Uniprot
P51813 (BMX) Y224 Uniprot
P51813 (BMX) Y566 Uniprot
P56945 (BCAR1) Y12 Uniprot
Q05397 (PTK2) Y194 Uniprot
Q05397 (PTK2) Y441 Uniprot
Q05397 (PTK2) Y576 Uniprot
Q05397 (PTK2) Y577 Uniprot
Q05397 (PTK2) Y742 Uniprot
Q05397 (PTK2) Y861 Uniprot
Q05397 (PTK2) Y898 Uniprot
Q05397 (PTK2) Y925 Uniprot
Q05397 (PTK2) Y1007 Uniprot
Q07912 (TNK2) Y859 Uniprot
Q07912 (TNK2) Y860 Uniprot
Q16611 (BAK1) Y108 Uniprot
Q96T51 (RUFY1) Y389 Uniprot
Q96T51 (RUFY1) Y400 Uniprot

研究背景

功能:

Non-receptor tyrosine kinase that plays central but diverse modulatory roles in various signaling processes involved in the regulation of actin reorganization, cell migration, cell proliferation and survival, cell adhesion, and apoptosis. Participates in signal transduction stimulated by growth factor receptors, cytokine receptors, G-protein coupled receptors, antigen receptors and integrins. Induces tyrosine phosphorylation of BCAR1 in response to integrin regulation. Activation of BMX by integrins is mediated by PTK2/FAK1, a key mediator of integrin signaling events leading to the regulation of actin cytoskeleton and cell motility. Plays a critical role in TNF-induced angiogenesis, and implicated in the signaling of TEK and FLT1 receptors, 2 important receptor families essential for angiogenesis. Required for the phosphorylation and activation of STAT3, a transcription factor involved in cell differentiation. Also involved in interleukin-6 (IL6) induced differentiation. Plays also a role in programming adaptive cytoprotection against extracellular stress in different cell systems, salivary epithelial cells, brain endothelial cells, and dermal fibroblasts. May be involved in regulation of endocytosis through its interaction with an endosomal protein RUFY1. May also play a role in the growth and differentiation of hematopoietic cells; as well as in signal transduction in endocardial and arterial endothelial cells.

翻译修饰:

Phosphorylated in response to protein I/II and to LPS. Phosphorylation at Tyr-566 by SRC and by autocatalysis leads to activation and is required for STAT3 phosphorylation by BMX.

细胞定位:

Cytoplasm.
Note: Localizes to the edges of spreading cells when complexed with BCAR1.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Highly expressed in cells with great migratory potential, including endothelial cells and metastatic carcinoma cell lines.

亚基结构:

Interacts with BCAR1, CAV1, MYD88, PTK2/FAK1, RUFY1, RUFY2, STAT3, TIRAP and TNFRSF1B.

蛋白家族:

SH2 domain mediates interaction with RUFY1.

Belongs to the protein kinase superfamily. Tyr protein kinase family. TEC subfamily.

限制条款

产品的规格、报价、验证数据请以官网为准,官网链接:www.affbiotech.com | www.affbiotech.cn(简体中文)| www.affbiotech.jp(日本語)

产品的数据信息为Affinity所有,未经授权不得收集Affinity官网数据或资料用于商业用途,对抄袭产品数据的行为我们将保留诉诸法律的权利。

产品相关数据会因产品批次、产品检测情况随时调整,如您已订购该产品,请以订购时随货说明书为准,否则请以官网内容为准,官网内容有改动时恕不另行通知。

Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时,请您在Affinity官方渠道提交售后申请。

产品仅供科学研究使用。不用于诊断和治疗。 

产品未经授权不得转售。

Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.