| 产品: | 磷酸化 PSMC5 (Ser120) 抗体 |
| 货号: | AF4452 |
| 描述: | Rabbit polyclonal antibody to Phospho-PSMC5 (Ser120) |
| 应用: | WB |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 46kDa; 46kD(Calculated). |
| 蛋白号: | P62195 |
| RRID: | AB_2844511 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF4452, RRID:AB_2844511.
展开/折叠
26S protease regulatory subunit 8; 26S proteasome AAA-ATPase subunit RPT6; Cim3; MSUG1 protein; p45; p45/SUG; Proteasome 26S ATPase subunit 5; Proteasome 26S subunit ATPase 5; Proteasome prosome macropain 26S subunit ATPase 5; Proteasome subunit p45; PRS8_HUMAN; PSMC5; Rpt6; S8; SUG1; Tat binding protein homolog 10; TBP10; Thyroid hormone receptor interacting protein 1; Thyroid hormone receptor-interacting protein 1; Thyroid receptor interactor 1; TRIP1; TRIP1(SUG1);
抗原和靶标
- P62195 PRS8_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MALDGPEQMELEEGKAGSGLRQYYLSKIEELQLIVNDKSQNLRRLQAQRNELNAKVRLLREELQLLQEQGSYVGEVVRAMDKKKVLVKVHPEGKFVVDVDKNIDINDVTPNCRVALRNDSYTLHKILPNKVDPLVSLMMVEKVPDSTYEMIGGLDKQIKEIKEVIELPVKHPELFEALGIAQPKGVLLYGPPGTGKTLLARAVAHHTDCTFIRVSGSELVQKFIGEGARMVRELFVMAREHAPSIIFMDEIDSIGSSRLEGGSGGDSEVQRTMLELLNQLDGFEATKNIKVIMATNRIDILDSALLRPGRIDRKIEFPPPNEEARLDILKIHSRKMNLTRGINLRKIAELMPGASGAEVKGVCTEAGMYALRERRVHVTQEDFEMAVAKVMQKDSEKNMSIKKLWK
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P62195 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| K15 | Ubiquitination | Uniprot | |
| R21 | Methylation | Uniprot | |
| Y24 | Phosphorylation | Uniprot | |
| S26 | Phosphorylation | Uniprot | |
| K27 | Ubiquitination | Uniprot | |
| K38 | Ubiquitination | Uniprot | |
| K55 | Ubiquitination | Uniprot | |
| Y72 | Phosphorylation | Uniprot | |
| K88 | Ubiquitination | Uniprot | |
| K94 | Acetylation | Uniprot | |
| K94 | Ubiquitination | Uniprot | |
| K101 | Ubiquitination | Uniprot | |
| T109 | Phosphorylation | Uniprot | |
| C112 | S-Nitrosylation | Uniprot | |
| S120 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| Y121 | Phosphorylation | Uniprot | |
| K125 | Ubiquitination | Uniprot | |
| K130 | Ubiquitination | Uniprot | |
| S136 | Phosphorylation | Uniprot | |
| K142 | Ubiquitination | Uniprot | |
| T147 | Phosphorylation | Uniprot | |
| Y148 | Phosphorylation | Uniprot | |
| K156 | Ubiquitination | Uniprot | |
| K162 | Ubiquitination | Uniprot | |
| K170 | Ubiquitination | Uniprot | |
| K184 | Ubiquitination | Uniprot | |
| Y189 | Phosphorylation | Uniprot | |
| K196 | Ubiquitination | Uniprot | |
| T197 | Phosphorylation | Uniprot | |
| S217 | Phosphorylation | Uniprot | |
| K222 | Acetylation | Uniprot | |
| K222 | Ubiquitination | Uniprot | |
| S244 | Phosphorylation | Uniprot | |
| S256 | Phosphorylation | Uniprot | |
| S257 | Phosphorylation | Uniprot | |
| S263 | Phosphorylation | Uniprot | |
| K287 | Ubiquitination | Uniprot | |
| K290 | Ubiquitination | Uniprot | |
| S303 | Phosphorylation | Uniprot | |
| K314 | Ubiquitination | Uniprot | |
| K330 | Ubiquitination | Uniprot | |
| K346 | Ubiquitination | Uniprot | |
| K360 | Ubiquitination | Uniprot | |
| T364 | Phosphorylation | Uniprot | |
| K389 | Ubiquitination | Uniprot | |
| K397 | Ubiquitination | Uniprot | |
| K402 | Ubiquitination | Uniprot | |
| K403 | Ubiquitination | Uniprot |
研究背景
Component of the 26S proteasome, a multiprotein complex involved in the ATP-dependent degradation of ubiquitinated proteins. This complex plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins, which could impair cellular functions, and by removing proteins whose functions are no longer required. Therefore, the proteasome participates in numerous cellular processes, including cell cycle progression, apoptosis, or DNA damage repair. PSMC5 belongs to the heterohexameric ring of AAA (ATPases associated with diverse cellular activities) proteins that unfolds ubiquitinated target proteins that are concurrently translocated into a proteolytic chamber and degraded into peptides.
Cytoplasm. Nucleus.
Component of the 19S proteasome regulatory particle complex. The 26S proteasome consists of a 20S core particle (CP) and two 19S regulatory subunits (RP). The regulatory particle is made of a lid composed of 9 subunits, a base containing 6 ATPases including PSMC5 and few additional components. Component of a complex with USP49 and RUVBL1. Interacts with PRPF19. Interacts with TRIM5. Interacts with NDC80. Interacts with PAAF1. Interacts, in vitro, with the thyroid hormone receptor (in a thyroid hormone T3-dependent manner) and with retinoid X receptor (RXR) (By similarity). Interacts with ERCC6.
Belongs to the AAA ATPase family.
研究领域
· Genetic Information Processing > Folding, sorting and degradation > Proteasome.
· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.
限制条款
产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。
产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。
Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。产品仅供科学研究使用。不用于诊断和治疗。
产品未经授权不得转售。
Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.