| 产品: | 磷酸化 CDC20 (Ser51) 抗体 |
| 货号: | AF4459 |
| 描述: | Rabbit polyclonal antibody to Phospho-CDC20 (Ser51) |
| 应用: | WB |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Bovine, Horse, Rabbit, Dog |
| 分子量: | 50kDa; 55kD(Calculated). |
| 蛋白号: | Q12834 |
| RRID: | AB_2844516 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF4459, RRID:AB_2844516.
展开/折叠
bA276H19.3; Cdc 20; CDC20; CDC20 cell division cycle 20 homolog; CDC20_HUMAN; CDC20A; Cell division cycle 20; Cell division cycle 20 homolog (S. cerevisiae); Cell division cycle 20 homolog; Cell division cycle protein 20 homolog; fizzy; MGC102824; p55CDC;
抗原和靶标
- Q12834 CDC20_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAQFAFESDLHSLLQLDAPIPNAPPARWQRKAKEAAGPAPSPMRAANRSHSAGRTPGRTPGKSSSKVQTTPSKPGGDRYIPHRSAAQMEVASFLLSKENQPENSQTPTKKEHQKAWALNLNGFDVEEAKILRLSGKPQNAPEGYQNRLKVLYSQKATPGSSRKTCRYIPSLPDRILDAPEIRNDYYLNLVDWSSGNVLAVALDNSVYLWSASSGDILQLLQMEQPGEYISSVAWIKEGNYLAVGTSSAEVQLWDVQQQKRLRNMTSHSARVGSLSWNSYILSSGSRSGHIHHHDVRVAEHHVATLSGHSQEVCGLRWAPDGRHLASGGNDNLVNVWPSAPGEGGWVPLQTFTQHQGAVKAVAWCPWQSNVLATGGGTSDRHIRIWNVCSGACLSAVDAHSQVCSILWSPHYKELISGHGFAQNQLVIWKYPTMAKVAELKGHTSRVLSLTMSPDGATVASAAADETLRLWRCFELDPARRREREKASAAKSSLIHQGIR
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q12834 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Ubiquitination | Uniprot | ||
| K33 | Methylation | Uniprot | |
| S41 | Phosphorylation | PR:O43683 (hBUB1) , O43683 (BUB1) | Uniprot |
| S49 | Phosphorylation | Uniprot | |
| S51 | Phosphorylation | Uniprot | |
| T55 | Phosphorylation | Uniprot | |
| T59 | Phosphorylation | Uniprot | |
| K62 | Ubiquitination | Uniprot | |
| S63 | Phosphorylation | Uniprot | |
| S64 | Phosphorylation | Uniprot | |
| K66 | Acetylation | Uniprot | |
| K66 | Ubiquitination | Uniprot | |
| T69 | Phosphorylation | Uniprot | |
| T70 | Phosphorylation | Uniprot | |
| S72 | Phosphorylation | PR:O43683 (hBUB1) , O43683 (BUB1) | Uniprot |
| K73 | Ubiquitination | Uniprot | |
| Y79 | Phosphorylation | Uniprot | |
| S84 | Phosphorylation | Uniprot | |
| S92 | Phosphorylation | O43683 (BUB1) , PR:O43683 (hBUB1) | Uniprot |
| S96 | Phosphorylation | Uniprot | |
| K97 | Ubiquitination | Uniprot | |
| T106 | Phosphorylation | Uniprot | |
| K109 | Sumoylation | Uniprot | |
| K109 | Ubiquitination | Uniprot | |
| K114 | Ubiquitination | Uniprot | |
| K129 | Ubiquitination | Uniprot | |
| S134 | Phosphorylation | Uniprot | |
| K136 | Ubiquitination | Uniprot | |
| K149 | Ubiquitination | Uniprot | |
| Y152 | Phosphorylation | Uniprot | |
| S153 | Phosphorylation | O43683 (BUB1) , PR:O43683 (hBUB1) | Uniprot |
| K155 | Ubiquitination | Uniprot | |
| T157 | Phosphorylation | O43683 (BUB1) , PR:O43683 (hBUB1) | Uniprot |
| S160 | Phosphorylation | Uniprot | |
| S161 | Phosphorylation | PR:O43683 (hBUB1) , O43683 (BUB1) | Uniprot |
| T164 | Phosphorylation | Uniprot | |
| S170 | Phosphorylation | P53350 (PLK1) | Uniprot |
| R174 | Methylation | Uniprot | |
| K259 | Ubiquitination | Uniprot | |
| S285 | Phosphorylation | Uniprot | |
| K435 | Ubiquitination | Uniprot | |
| K440 | Ubiquitination | Uniprot | |
| S448 | Phosphorylation | Uniprot | |
| T457 | Phosphorylation | Uniprot | |
| T466 | Phosphorylation | Uniprot | |
| K485 | Ubiquitination | Uniprot | |
| S487 | Phosphorylation | Uniprot | |
| K490 | Sumoylation | Uniprot | |
| K490 | Ubiquitination | Uniprot | |
| S492 | Phosphorylation | Uniprot |
研究背景
Required for full ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) and may confer substrate specificity upon the complex. Is regulated by MAD2L1: in metaphase the MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the CDC20-APC/C binary complex is active in degrading substrates. The CDC20-APC/C complex positively regulates the formation of synaptic vesicle clustering at active zone to the presynaptic membrane in postmitotic neurons. CDC20-APC/C-induced degradation of NEUROD2 induces presynaptic differentiation.
Acetylated. Deacetylated at Lys-66 by SIRT2; deacetylation enhances the interaction of CDC20 with CDC27, leading to activation of anaphase promoting complex/cyclosome (APC/C).
Phosphorylated during mitosis, probably by maturation promoting factor (MPF). Phosphorylated by BUB1 at Ser-41; Ser-72; Ser-92; Ser-153; Thr-157 and Ser-161. Phosphorylated by NEK2.
Dephosphorylated by CTDP1.
Ubiquitinated and degraded by the proteasome during spindle assembly checkpoint. Deubiquitinated by USP44, leading to stabilize the MAD2L1-CDC20-APC/C ternary complex, thereby preventing premature activation of the APC/C. Ubiquitinated at Lys-490 during prometaphase. Ubiquitination at Lys-485 and Lys-490 has no effect on its ability to bind the APC/C complex.
Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Cytoplasm>Cytoskeleton>Spindle pole.
Found in a complex with CDC20, CDC27, SPATC1 and TUBG1. Interacts with NEUROD2 and SPATC1 (By similarity). Interacts with MAD2L1 and BUB1B. The phosphorylated form interacts with APC/C. Interacts with NINL. May interact with MAD2L2. Interacts with CDK5RAP2 and SIRT2. Interacts with isoform 1 of NEK2. Interacts with HSF1 (via phosphorylated form); this interaction occurs in mitosis in a MAD2L1-dependent manner and prevents PLK1-stimulated degradation of HSF1 by blocking the recruitment of the SCF(BTRC) ubiquitin ligase complex. Interacts (via the N-terminal substrate-binding domain) with FBXO5 (By similarity).
Belongs to the WD repeat CDC20/Fizzy family.
研究领域
· Cellular Processes > Cell growth and death > Cell cycle. (View pathway)
· Cellular Processes > Cell growth and death > Oocyte meiosis. (View pathway)
· Genetic Information Processing > Folding, sorting and degradation > Ubiquitin mediated proteolysis. (View pathway)
· Human Diseases > Infectious diseases: Viral > HTLV-I infection.
· Human Diseases > Cancers: Overview > Viral carcinogenesis.
限制条款
产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。
产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。
Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。产品仅供科学研究使用。不用于诊断和治疗。
产品未经授权不得转售。
Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.