Phospho-Scribble (Ser1220) Antibody - #AF4471

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产品: 磷酸化 Scribble (Ser1220) 抗体
货号: AF4471
描述: Rabbit polyclonal antibody to Phospho-Scribble (Ser1220)
应用: WB
反应: Human, Mouse
预测: Pig, Bovine, Horse
分子量: 240kDa; 175kD(Calculated).
蛋白号: Q14160
RRID: AB_2844525

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse
预测:
Pig(83%), Bovine(83%), Horse(92%)
克隆:
Polyclonal
特异性:
Phospho-Scribble (Ser1220) Antibody detects endogenous levels of Scribble only when phosphorylated at Ser1220.
RRID:
AB_2844525
引用格式: Affinity Biosciences Cat# AF4471, RRID:AB_2844525.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

CRIB 1; CRIB1; hScrib; LAP 4; LAP4; PDZ domain protein scribble; Protein LAP4; Protein scribble homolog; SCRB 1; SCRB1; SCRIB 1; Scrib; SCRIB_HUMAN; SCRIB1; Scribble; Scribble homolog 1; scribbled homolog (Drosophila); Vartul;

抗原和靶标

免疫原:
Uniprot:

Q14160(SCRIB_HUMAN) >>Visit HPA database.

基因/基因ID:
SCRIB(23513)
表达:
Q14160 SCRIB_HUMAN:

Expressed in kidney, skeletal muscles, liver, lung, breast, intestine, placenta and skin mainly in epithelial cells (at protein level).

序列:
MLKCIPLWRCNRHVESVDKRHCSLQAVPEEIYRYSRSLEELLLDANQLRELPKPFFRLLNLRKLGLSDNEIQRLPPEVANFMQLVELDVSRNDIPEIPESIKFCKALEIADFSGNPLSRLPDGFTQLRSLAHLALNDVSLQALPGDVGNLANLVTLELRENLLKSLPASLSFLVKLEQLDLGGNDLEVLPDTLGALPNLRELWLDRNQLSALPPELGNLRRLVCLDVSENRLEELPAELGGLVLLTDLLLSQNLLRRLPDGIGQLKQLSILKVDQNRLCEVTEAIGDCENLSELILTENLLMALPRSLGKLTKLTNLNVDRNHLEALPPEIGGCVALSVLSLRDNRLAVLPPELAHTTELHVLDVAGNRLQSLPFALTHLNLKALWLAENQAQPMLRFQTEDDARTGEKVLTCYLLPQQPPPSLEDAGQQGSLSETWSDAPPSRVSVIQFLEAPIGDEDAEEAAAEKRGLQRRATPHPSELKVMKRSIEGRRSEACPCQPDSGSPLPAEEEKRLSAESGLSEDSRPSASTVSEAEPEGPSAEAQGGSQQEATTAGGEEDAEEDYQEPTVHFAEDALLPGDDREIEEGQPEAPWTLPGGRQRLIRKDTPHYKKHFKISKLPQPEAVVALLQGMQPDGEGPVAPGGWHNGPHAPWAPRAQKEEEEEEEGSPQEEEVEEEEENRAEEEEASTEEEDKEGAVVSAPSVKGVSFDQANNLLIEPARIEEEELTLTILRQTGGLGISIAGGKGSTPYKGDDEGIFISRVSEEGPAARAGVRVGDKLLEVNGVALQGAEHHEAVEALRGAGTAVQMRVWRERMVEPENAVTITPLRPEDDYSPRERRGGGLRLPLLPPESPGPLRQRHVACLARSERGLGFSIAGGKGSTPYRAGDAGIFVSRIAEGGAAHRAGTLQVGDRVLSINGVDVTEARHDHAVSLLTAASPTIALLLEREAGGPLPPSPLPHSSPPTAAVATTSITTATPGVPGLPSLAPSLLAAALEGPYPVEEIRLPRAGGPLGLSIVGGSDHSSHPFGVQEPGVFISKVLPRGLAARSGLRVGDRILAVNGQDVRDATHQEAVSALLRPCLELSLLVRRDPAPPGLRELCIQKAPGERLGISIRGGARGHAGNPRDPTDEGIFISKVSPTGAAGRDGRLRVGLRLLEVNQQSLLGLTHGEAVQLLRSVGDTLTVLVCDGFEASTDAALEVSPGVIANPFAAGIGHRNSLESISSIDRELSPEGPGKEKELPGQTLHWGPEATEAAGRGLQPLKLDYRALAAVPSAGSVQRVPSGAAGGKMAESPCSPSGQQPPSPPSPDELPANVKQAYRAFAAVPTSHPPEDAPAQPPTPGPAASPEQLSFRERQKYFELEVRVPQAEGPPKRVSLVGADDLRKMQEEEARKLQQKRAQMLREAAEAGAEARLALDGETLGEEEQEDEQPPWASPSPTSRQSPASPPPLGGGAPVRTAKAERRHQERLRVQSPEPPAPERALSPAELRALEAEKRALWRAARMKSLEQDALRAQMVLSRSQEGRGTRGPLERLAEAPSPAPTPSPTPVEDLGPQTSTSPGRLSPDFAEELRSLEPSPSPGPQEEDGEVALVLLGRPSPGAVGPEDVALCSSRRPVRPGRRGLGPVPS

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Horse
92
Pig
83
Bovine
83
Dog
75
Zebrafish
67
Sheep
55
Xenopus
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q14160 作为底物

Site PTM Type Enzyme
K19 Ubiquitination
C22 S-Nitrosylation
S23 Phosphorylation
Y32 Phosphorylation
Y34 Phosphorylation
S35 Phosphorylation
S37 Phosphorylation
K53 Ubiquitination
K63 Ubiquitination
S67 Phosphorylation
K102 Ubiquitination
K105 Ubiquitination
K164 Ubiquitination
S165 Phosphorylation
K266 Ubiquitination
K272 Ubiquitination
K313 Ubiquitination
T378 Phosphorylation
S432 Phosphorylation
S446 Phosphorylation
K467 Ubiquitination
T475 Phosphorylation
S487 Phosphorylation
S493 Phosphorylation
S504 Phosphorylation
K512 Ubiquitination
S515 Phosphorylation
T553 Phosphorylation P27361 (MAPK3)
Y564 Phosphorylation
T607 Phosphorylation
Y610 Phosphorylation
S668 Phosphorylation
S688 Phosphorylation
T689 Phosphorylation
S703 Phosphorylation
K705 Ubiquitination
S708 Phosphorylation
T728 Phosphorylation
T730 Phosphorylation
T735 Phosphorylation
S741 Phosphorylation
S748 Phosphorylation
T749 Phosphorylation
K752 Ubiquitination
S764 Phosphorylation
T824 Phosphorylation
T826 Phosphorylation
Y834 Phosphorylation
S835 Phosphorylation
S853 Phosphorylation
S868 Phosphorylation
S875 Phosphorylation
K880 Ubiquitination
T883 Phosphorylation
R886 Methylation
S917 Phosphorylation
S939 Phosphorylation
T941 Phosphorylation
Y1000 Phosphorylation
S1017 Phosphorylation
K1040 Ubiquitination
S1050 Phosphorylation
K1105 Ubiquitination
S1114 Phosphorylation
R1116 Methylation
R1120 Methylation
K1138 Ubiquitination
S1140 Phosphorylation
S1220 Phosphorylation
S1223 Phosphorylation
S1225 Phosphorylation
S1226 Phosphorylation
S1232 Phosphorylation
K1240 Ubiquitination
R1259 Methylation
K1265 Ubiquitination
Y1268 Phosphorylation
S1276 Phosphorylation
S1279 Phosphorylation
S1285 Phosphorylation
S1295 Phosphorylation
S1298 Phosphorylation
S1300 Phosphorylation
S1306 Phosphorylation
S1309 Phosphorylation
T1342 Phosphorylation
S1348 Phosphorylation
S1353 Phosphorylation
Y1360 Phosphorylation
S1378 Phosphorylation Q13464 (ROCK1)
S1437 Phosphorylation
S1439 Phosphorylation
S1442 Phosphorylation
S1445 Phosphorylation
S1448 Phosphorylation P27361 (MAPK3)
T1460 Phosphorylation
S1475 Phosphorylation
S1486 Phosphorylation
K1507 Methylation
S1508 Phosphorylation Q13464 (ROCK1)
S1521 Phosphorylation
S1523 Phosphorylation
R1527 Methylation
T1529 Phosphorylation
S1541 Phosphorylation
T1545 Phosphorylation
S1547 Phosphorylation
T1549 Phosphorylation
T1558 Phosphorylation
S1559 Phosphorylation
T1560 Phosphorylation
S1561 Phosphorylation
S1566 Phosphorylation
S1579 Phosphorylation
S1581 Phosphorylation
S1600 Phosphorylation
S1613 Phosphorylation
S1630 Phosphorylation

研究背景

功能:

Scaffold protein involved in different aspects of polarized cells differentiation regulating epithelial and neuronal morphogenesis. Most probably functions in the establishment of apico-basal cell polarity. May function in cell proliferation regulating progression from G1 to S phase and as a positive regulator of apoptosis for instance during acinar morphogenesis of the mammary epithelium. May also function in cell migration and adhesion and hence regulate cell invasion through MAPK signaling. May play a role in exocytosis and in the targeting synaptic vesicles to synapses. Functions as an activator of Rac GTPase activity.

翻译修饰:

Ubiquitinated; targeted for UBE3A-dependent multiubiquitination in the presence of high-risk HPV E6 proteins and degraded.

细胞定位:

Cell membrane>Peripheral membrane protein. Cell junction>Adherens junction. Cell projection>Lamellipodium. Cytoplasm. Cell junction>Synapse>Postsynapse. Cell junction>Synapse>Presynapse.
Note: Targeting to cell-cell junctions which is CDH1-dependent is required for the pro-apoptotic activity.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed in kidney, skeletal muscles, liver, lung, breast, intestine, placenta and skin mainly in epithelial cells (at protein level).

亚基结构:

Interacts (via PDZ domains) with VANGL2. Interacts with CTNNB1 and MAPK12 (By similarity). Interacts with UBE3A and HPV E6. Interacts with PAK1 and PAK2. Interacts with ARHGEF7 and GIT1; interacts directly with ARHGEF7. Interacts (via PDZ domains) with LPP and TRIP6; the interaction is direct. Interacts (via PDZ domains) with TJP2. Interacts (via PDZ domains) with APC; may mediate APC targeting to adherens junctions of epithelial cells. Interacts (via PDZ domains) with TSHR; regulates TSHR trafficking and function. Interacts (via PDZ domains 1 and 3) with MCC. Interacts (via fourth PDZ domain) with tick-borne encephalitis virus NS5 protein; this interaction inhibits SCRIB and downstream STAT1 phosphorylation, thereby preventing activation of JAK-STAT signaling pathway. Interacts with DLG5. Interacts with STK4/MST1 and LATS1 in the presence of DLG5.

(Microbial infection) Interacts with tick-borne encephalitis virus RNA-directed RNA polymerase NS5; this interaction targets viral NS5 to the cell membrane periphery and nucleus, thereby allowing efficient host nuclear STAT1 inhibition.

蛋白家族:

Belongs to the LAP (LRR and PDZ) protein family.

研究领域

· Cellular Processes > Cellular community - eukaryotes > Tight junction.   (View pathway)

· Environmental Information Processing > Signal transduction > Hippo signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

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