Phospho-IRF4 (Tyr122/Tyr125) Antibody - #AF4476
| 产品: | 磷酸化 IRF4 (Tyr122/Tyr125) 抗体 |
| 货号: | AF4476 |
| 描述: | Rabbit polyclonal antibody to Phospho-IRF4 (Tyr122/Tyr125) |
| 应用: | WB IHC |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Bovine, Sheep, Rabbit, Dog |
| 分子量: | 51kDa; 52kD(Calculated). |
| 蛋白号: | Q15306 |
| RRID: | AB_2844529 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF4476, RRID:AB_2844529.
展开/折叠
Interferon regulatory factor 4; IRF 4; IRF-4; Irf4; IRF4_HUMAN; LSIRF; Lymphocyte specific interferon regulatory factor; Lymphocyte specific IRF; Lymphocyte-specific interferon regulatory factor; Multiple myeloma oncogene 1; MUM 1; MUM1; NF EM5; NF-EM5; NFEM5; PU.1 interaction partner; Sfpi1/PU.1 interaction partner; Transcriptional activator PIP;
抗原和靶标
- Q15306 IRF4_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MNLEGGGRGGEFGMSAVSCGNGKLRQWLIDQIDSGKYPGLVWENEEKSIFRIPWKHAGKQDYNREEDAALFKAWALFKGKFREGIDKPDPPTWKTRLRCALNKSNDFEELVERSQLDISDPYKVYRIVPEGAKKGAKQLTLEDPQMSMSHPYTMTTPYPSLPAQQVHNYMMPPLDRSWRDYVPDQPHPEIPYQCPMTFGPRGHHWQGPACENGCQVTGTFYACAPPESQAPGVPTEPSIRSAEALAFSDCRLHICLYYREILVKELTTSSPEGCRISHGHTYDASNLDQVLFPYPEDNGQRKNIEKLLSHLERGVVLWMAPDGLYAKRLCQSRIYWDGPLALCNDRPNKLERDQTCKLFDTQQFLSELQAFAHHGRSLPRFQVTLCFGEEFPDPQRQRKLITAHVEPLLARQLYYFAQQNSGHFLRGYDLPEHISNPEDYHRSIRHSSIQE
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q15306 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K36 | Ubiquitination | Uniprot | |
| Y37 | Phosphorylation | Uniprot | |
| S48 | Phosphorylation | Uniprot | |
| K59 | Ubiquitination | Uniprot | |
| Y62 | Phosphorylation | Uniprot | |
| K103 | Acetylation | Uniprot | |
| K103 | Ubiquitination | Uniprot | |
| Y122 | Phosphorylation | Uniprot | |
| K123 | Ubiquitination | Uniprot | |
| Y125 | Phosphorylation | Uniprot | |
| S177 | Phosphorylation | Uniprot | |
| Y181 | Phosphorylation | Uniprot | |
| Y192 | Phosphorylation | Uniprot | |
| R201 | Methylation | Uniprot | |
| K264 | Ubiquitination | Uniprot | |
| K306 | Ubiquitination | Uniprot | |
| Y325 | Phosphorylation | Uniprot | |
| K327 | Ubiquitination | Uniprot | |
| K349 | Ubiquitination | Uniprot | |
| K357 | Ubiquitination | Uniprot | |
| K399 | Ubiquitination | Uniprot | |
| Y428 | Phosphorylation | Uniprot | |
| Y440 | Phosphorylation | Uniprot | |
| S447 | Phosphorylation | O75116 (ROCK2) | Uniprot |
| S448 | Phosphorylation | O75116 (ROCK2) | Uniprot |
研究背景
Transcriptional activator. Binds to the interferon-stimulated response element (ISRE) of the MHC class I promoter. Binds the immunoglobulin lambda light chain enhancer, together with PU.1. Probably plays a role in ISRE-targeted signal transduction mechanisms specific to lymphoid cells. Involved in CD8(+) dendritic cell differentiation by forming a complex with the BATF-JUNB heterodimer in immune cells, leading to recognition of AICE sequence (5'-TGAnTCA/GAAA-3'), an immune-specific regulatory element, followed by cooperative binding of BATF and IRF4 and activation of genes (By similarity).
Phosphorylation by ROCK2 regulates IL-17 and IL-21 production.
Nucleus.
Lymphoid cells.
Interacts with the BATF-JUNB heterodimer. Interacts with BATF (via bZIP domain); the interaction is direct (By similarity). Interacts with SPIB and DEF6. Directly interacts with NLRP3 in the nucleus of Th2 cells; this interaction enhances IRF4 ability to bind to the IL4 promoter and is required for optimal IRF4-dependent IL4 transcription (By similarity).
Belongs to the IRF family.
研究领域
· Organismal Systems > Immune system > Th17 cell differentiation. (View pathway)
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