| 产品: | 磷酸化 GKAP (Ser25) 抗体 |
| 货号: | AF4479 |
| 描述: | Rabbit polyclonal antibody to Phospho-GKAP (Ser25) |
| 应用: | WB IHC |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Bovine, Horse, Sheep, Rabbit, Dog |
| 分子量: | 90-150kDa; 42kD(Calculated). |
| 蛋白号: | Q5VSY0 |
| RRID: | AB_2844530 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF4479, RRID:AB_2844530.
展开/折叠
cGMP dependent protein kinase anchoring protein 42kDa; cGMP dependent protein kinase anchoring protein of 42 kDa; cGMP-dependent protein kinase-anchoring protein of 42 kDa; FKSG21; G kinase anchoring protein 1; G kinase-anchoring protein 1; gkap1; GKAP1_HUMAN; GKAP42; Protein kinase anchoring protein GKAP42;
抗原和靶标
- Q5VSY0 GKAP1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MASAVLSSVPTTASRFALLQVDSGSGSDSEPGKGKGRNTGKSQTLGSKSTTNEKKREKRRKKKEQQQSEANELRNLAFKKIPQKSSHAVCNAQHDLPLSNPVQKDSREENWQEWRQRDEQLTSEMFEADLEKALLLSKLEYEEHKKEYEDAENTSTQSKVMNKKDKRKNHQGKDRPLTVSLKDFHSEDHISKKTEELSSSQTLSHDGGFFNRLEDDVHKILIREKRREQLTEYNGTDNCTAHEHNQEVVLKDGRIERLKLELERKDAEIQKLKNVITQWEAKYKEVKARNAQLLKMLQEGEMKDKAEILLQVDESQSIKNELTIQVTSLHAALEQERSKVKVLQAELAKYQGGRKGKRNSESDQCR
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q5VSY0 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S3 | Phosphorylation | Uniprot | |
| S8 | Phosphorylation | Uniprot | |
| T12 | Phosphorylation | Uniprot | |
| S23 | Phosphorylation | Uniprot | |
| S25 | Phosphorylation | Uniprot | |
| S27 | Phosphorylation | Uniprot | |
| S29 | Phosphorylation | Uniprot | |
| T39 | Phosphorylation | Uniprot | |
| S42 | Phosphorylation | Uniprot | |
| T44 | Phosphorylation | Uniprot | |
| K58 | Acetylation | Uniprot | |
| K79 | Ubiquitination | Uniprot | |
| K104 | Ubiquitination | Uniprot | |
| Y141 | Phosphorylation | Uniprot | |
| Y148 | Phosphorylation | Uniprot | |
| T156 | Phosphorylation | Uniprot | |
| S158 | Phosphorylation | Uniprot | |
| K219 | Ubiquitination | Uniprot | |
| K259 | Ubiquitination | Uniprot | |
| K271 | Ubiquitination | Uniprot | |
| K282 | Ubiquitination | Uniprot | |
| K295 | Acetylation | Uniprot | |
| K303 | Acetylation | Uniprot | |
| T323 | Phosphorylation | Uniprot | |
| T327 | Phosphorylation | Uniprot | |
| S328 | Phosphorylation | Uniprot | |
| S360 | Phosphorylation | Uniprot |
研究背景
Regulates insulin-dependent IRS1 tyrosine phosphorylation in adipocytes by modulating the availability of IRS1 to IR tyrosine kinase. Its association with IRS1 is required for insulin-induced translocation of SLC2A4 to the cell membrane. Involved in TNF-induced impairment of insulin-dependent IRS1 tyrosine phosphorylation.
Golgi apparatus.
Interacts with PRKG1 and IRS1.
Belongs to the GKAP1 family.
限制条款
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