Phospho-BACH1/BRIP1 (Thr1133) Antibody - #AF4489

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产品: 磷酸化 BACH1/BRIP1 (Thr1133) 抗体
货号: AF4489
描述: Rabbit polyclonal antibody to Phospho-BACH1/BRIP1 (Thr1133)
应用: WB
反应: Human
预测: Pig, Bovine, Dog, Chicken
分子量: 145kDa; 141kD(Calculated).
蛋白号: Q9BX63
RRID: AB_2844540

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human
预测:
Pig(100%), Bovine(100%), Dog(92%), Chicken(91%)
克隆:
Polyclonal
特异性:
Phospho-BACH1/BRIP1 (Thr1133) Antibody detects endogenous levels of BACH1/BRIP1 only when phosphorylated at Thr1133.
RRID:
AB_2844540
引用格式: Affinity Biosciences Cat# AF4489, RRID:AB_2844540.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

ATP dependent RNA helicase BRIP1; ATP-dependent RNA helicase BRIP1; BACH 1; BRAC 1 Associated C Terminal Helicase 1; BRCA 1 Interacting Protein 1; BRCA1 binding helicase like protein BACH1; BRCA1 interacting protein C terminal helicase 1; BRCA1-associated C-terminal helicase 1; BRCA1-interacting protein 1; BRCA1-interacting protein C-terminal helicase 1; BRCA1/BRCA2 associated helicase 1; BRIP 1; BRIP1; FANCJ; FANCJ_HUMAN; Fanconi anemia group J protein; FLJ90232; MGC126521; MGC126523; OF; Protein FACJ;

抗原和靶标

免疫原:
Uniprot:

Q9BX63(FANCJ_HUMAN) >>Visit HPA database.

基因/基因ID:
BRIP1(83990)
表达:
Q9BX63 FANCJ_HUMAN:

Ubiquitously expressed, with highest levels in testis.

序列:
MSSMWSEYTIGGVKIYFPYKAYPSQLAMMNSILRGLNSKQHCLLESPTGSGKSLALLCSALAWQQSLSGKPADEGVSEKAEVQLSCCCACHSKDFTNNDMNQGTSRHFNYPSTPPSERNGTSSTCQDSPEKTTLAAKLSAKKQASIYRDENDDFQVEKKRIRPLETTQQIRKRHCFGTEVHNLDAKVDSGKTVKLNSPLEKINSFSPQKPPGHCSRCCCSTKQGNSQESSNTIKKDHTGKSKIPKIYFGTRTHKQIAQITRELRRTAYSGVPMTILSSRDHTCVHPEVVGNFNRNEKCMELLDGKNGKSCYFYHGVHKISDQHTLQTFQGMCKAWDIEELVSLGKKLKACPYYTARELIQDADIIFCPYNYLLDAQIRESMDLNLKEQVVILDEAHNIEDCARESASYSVTEVQLRFARDELDSMVNNNIRKKDHEPLRAVCCSLINWLEANAEYLVERDYESACKIWSGNEMLLTLHKMGITTATFPILQGHFSAVLQKEEKISPIYGKEEAREVPVISASTQIMLKGLFMVLDYLFRQNSRFADDYKIAIQQTYSWTNQIDISDKNGLLVLPKNKKRSRQKTAVHVLNFWCLNPAVAFSDINGKVQTIVLTSGTLSPMKSFSSELGVTFTIQLEANHIIKNSQVWVGTIGSGPKGRNLCATFQNTETFEFQDEVGALLLSVCQTVSQGILCFLPSYKLLEKLKERWLSTGLWHNLELVKTVIVEPQGGEKTNFDELLQVYYDAIKYKGEKDGALLVAVCRGKVSEGLDFSDDNARAVITIGIPFPNVKDLQVELKRQYNDHHSKLRGLLPGRQWYEIQAYRALNQALGRCIRHRNDWGALILVDDRFRNNPSRYISGLSKWVRQQIQHHSTFESALESLAEFSKKHQKVLNVSIKDRTNIQDNESTLEVTSLKYSTSPYLLEAASHLSPENFVEDEAKICVQELQCPKIITKNSPLPSSIISRKEKNDPVFLEEAGKAEKIVISRSTSPTFNKQTKRVSWSSFNSLGQYFTGKIPKATPELGSSENSASSPPRFKTEKMESKTVLPFTDKCESSNLTVNTSFGSCPQSETIISSLKIDATLTRKNHSEHPLCSEEALDPDIELSLVSEEDKQSTSNRDFETEAEDESIYFTPELYDPEDTDEEKNDLAETDRGNRLANNSDCILAKDLFEIRTIKEVDSAREVKAEDCIDTKLNGILHIEESKIDDIDGNVKTTWINELELGKTHEIEIKNFKPSPSKNKGMFPGFK

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Bovine
100
Dog
92
Chicken
91
Zebrafish
43
Horse
0
Sheep
0
Xenopus
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q9BX63 作为底物

Site PTM Type Enzyme
Y22 Phosphorylation
K39 Ubiquitination
S77 Phosphorylation
Y110 Phosphorylation
S112 Phosphorylation
T113 Phosphorylation
S116 Phosphorylation
T124 Phosphorylation
S128 Phosphorylation
K137 Ubiquitination
S139 Phosphorylation
K142 Ubiquitination
K158 Ubiquitination
K186 Acetylation
K191 Acetylation
S197 Phosphorylation
S204 Phosphorylation
S206 Phosphorylation
K222 Ubiquitination
S226 Phosphorylation
S229 Phosphorylation
S230 Phosphorylation
T232 Phosphorylation
K254 Ubiquitination
T274 Phosphorylation
S278 Phosphorylation
K297 Ubiquitination
K345 Ubiquitination
K348 Ubiquitination
Y461 Phosphorylation
K503 Ubiquitination
S505 Phosphorylation
Y508 Phosphorylation
K510 Ubiquitination
T584 Phosphorylation
K656 Ubiquitination
Y742 Phosphorylation
K747 Ubiquitination
K764 Ubiquitination
K797 Ubiquitination
K862 Ubiquitination
S895 Phosphorylation
K897 Ubiquitination
Y916 Phosphorylation
T918 Phosphorylation
Y921 Phosphorylation
S927 Phosphorylation
S930 Phosphorylation
T953 Phosphorylation
K954 Ubiquitination
S956 Phosphorylation
K982 Ubiquitination
S988 Phosphorylation
S990 Phosphorylation
S1001 Phosphorylation
S1004 Phosphorylation
S1007 Phosphorylation
T1020 Phosphorylation
S1031 Phosphorylation
S1032 Phosphorylation
T1045 Phosphorylation
T1050 Phosphorylation
S1063 Phosphorylation
T1133 Phosphorylation
T1142 Phosphorylation
S1162 Phosphorylation
K1168 Acetylation
K1168 Ubiquitination
K1214 Ubiquitination
S1237 Phosphorylation
S1239 Phosphorylation
K1249 Acetylation

研究背景

功能:

DNA-dependent ATPase and 5' to 3' DNA helicase required for the maintenance of chromosomal stability. Acts late in the Fanconi anemia pathway, after FANCD2 ubiquitination. Involved in the repair of DNA double-strand breaks by homologous recombination in a manner that depends on its association with BRCA1.

翻译修饰:

Phosphorylated. Phosphorylation is necessary for interaction with BRCA1, and is cell-cycle regulated.

Acetylation at Lys-1249 facilitates DNA end processing required for repair and checkpoint signaling.

细胞定位:

Nucleus. Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Ubiquitously expressed, with highest levels in testis.

亚基结构:

Binds directly to the BRCT domains of BRCA1. Interacts with the CIA complex components CIAO1, CIAO2B and MMS19.

蛋白家族:

4Fe-4S iron-sulfur-binding is required for helicase activity.

Belongs to the DEAD box helicase family. DEAH subfamily.

研究领域

· Genetic Information Processing > Replication and repair > Homologous recombination.

· Genetic Information Processing > Replication and repair > Fanconi anemia pathway.

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