| 产品: | 磷酸化 RanBP3 (Ser126) 抗体 |
| 货号: | AF4495 |
| 描述: | Rabbit polyclonal antibody to Phospho-RanBP3 (Ser126) |
| 应用: | WB |
| 反应: | Human |
| 预测: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 70kDa; 60kD(Calculated). |
| 蛋白号: | Q9H6Z4 |
| RRID: | AB_2844546 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF4495, RRID:AB_2844546.
展开/折叠
DKFZp586I1520; Ran binding protein 3; Ran-binding protein 3; RANB3_HUMAN; RanBP3;
抗原和靶标
- Q9H6Z4 RANB3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MADLANEEKPAIAPPVFVFQKDKGQKSPAEQKNLSDSGEEPRGEAEAPHHGTGHPESAGEHALEPPAPAGASASTPPPPAPEAQLPPFPRELAGRSAGGSSPEGGEDSDREDGNYCPPVKRERTSSLTQFPPSQSEERSSGFRLKPPTLIHGQAPSAGLPSQKPKEQQRSVLRPAVLQAPQPKALSQTVPSSGTNGVSLPADCTGAVPAASPDTAAWRSPSEAADEVCALEEKEPQKNESSNASEEEACEKKDPATQQAFVFGQNLRDRVKLINESVDEADMENAGHPSADTPTATNYFLQYISSSLENSTNSADASSNKFVFGQNMSERVLSPPKLNEVSSDANRENAAAESGSESSSQEATPEKESLAESAAAYTKATARKCLLEKVEVITGEEAESNVLQMQCKLFVFDKTSQSWVERGRGLLRLNDMASTDDGTLQSRLVMRTQGSLRLILNTKLWAQMQIDKASEKSIRITAMDTEDQGVKVFLISASSKDTGQLYAALHHRILALRSRVEQEQEAKMPAPEPGAAPSNEEDDSDDDDVLAPSGATAAGAGDEGDGQTTGST
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9H6Z4 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| K21 | Acetylation | Uniprot | |
| S27 | Phosphorylation | Uniprot | |
| S72 | Phosphorylation | Uniprot | |
| S74 | Phosphorylation | Uniprot | |
| T75 | Phosphorylation | Uniprot | |
| S96 | Phosphorylation | Uniprot | |
| S100 | Phosphorylation | Uniprot | |
| S101 | Phosphorylation | Uniprot | |
| S108 | Phosphorylation | Uniprot | |
| Y115 | Phosphorylation | Uniprot | |
| C116 | S-Nitrosylation | Uniprot | |
| T124 | Phosphorylation | Uniprot | |
| S125 | Phosphorylation | Uniprot | |
| S126 | Phosphorylation | P51812 (RPS6KA3) , P31749 (AKT1) , Q15418 (RPS6KA1) | Uniprot |
| T128 | Phosphorylation | Uniprot | |
| S133 | Phosphorylation | Uniprot | |
| K145 | Ubiquitination | Uniprot | |
| K163 | Acetylation | Uniprot | |
| S170 | Phosphorylation | Uniprot | |
| S186 | Phosphorylation | Uniprot | |
| T188 | Phosphorylation | Uniprot | |
| S198 | Phosphorylation | Uniprot | |
| C203 | S-Nitrosylation | Uniprot | |
| S211 | Phosphorylation | Uniprot | |
| T214 | Phosphorylation | Uniprot | |
| S219 | Phosphorylation | Uniprot | |
| S221 | Phosphorylation | Uniprot | |
| K233 | Ubiquitination | Uniprot | |
| S240 | Phosphorylation | Uniprot | |
| S241 | Phosphorylation | Uniprot | |
| S244 | Phosphorylation | Uniprot | |
| T256 | Phosphorylation | Uniprot | |
| S276 | Phosphorylation | Uniprot | |
| T292 | Phosphorylation | Uniprot | |
| Y298 | Phosphorylation | Uniprot | |
| S328 | Phosphorylation | Uniprot | |
| S333 | Phosphorylation | Uniprot | |
| S353 | Phosphorylation | Uniprot | |
| S355 | Phosphorylation | Uniprot | |
| S357 | Phosphorylation | Uniprot | |
| S358 | Phosphorylation | Uniprot | |
| S359 | Phosphorylation | Uniprot | |
| T363 | Phosphorylation | Uniprot | |
| S372 | Phosphorylation | Uniprot | |
| K378 | Ubiquitination | Uniprot | |
| K413 | Ubiquitination | Uniprot | |
| T457 | Phosphorylation | Uniprot | |
| S493 | Phosphorylation | Uniprot | |
| Y501 | Phosphorylation | Uniprot | |
| S533 | Phosphorylation | Uniprot | |
| S539 | Phosphorylation | Uniprot |
研究背景
Acts as a cofactor for XPO1/CRM1-mediated nuclear export, perhaps as export complex scaffolding protein. Bound to XPO1/CRM1, stabilizes the XPO1/CRM1-cargo interaction. In the absence of Ran-bound GTP prevents binding of XPO1/CRM1 to the nuclear pore complex. Binds to CHC1/RCC1 and increases the guanine nucleotide exchange activity of CHC1/RCC1. Recruits XPO1/CRM1 to CHC1/RCC1 in a Ran-dependent manner. Negative regulator of TGF-beta signaling through interaction with the R-SMAD proteins, SMAD2 and SMAD3, and mediating their nuclear export.
Cytoplasm. Nucleus.
Widely expressed with high levels in testis and heart.
Interacts with CHC1 in a Ran-stimulated manner. Interacts with XPO1. Interacts (via its C-terminal R domain) with SMAD2 (dephosphorylated form via its MH1 and MH2 domains); the interaction results in the nuclear export of SMAD2 and termination of the TGF-beta signaling. Interacts (via its C-terminal R domain) with SMAD3 (dephosphorylated form via its MH1 domain); the interaction results in the nuclear export of SMAD3 and termination of the TGF-beta signaling.
研究领域
· Human Diseases > Infectious diseases: Viral > HTLV-I infection.
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