产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF4497, RRID:AB_2844548.
展开/折叠
2810024C04Rik; DNA repair protein rad18; E3 ubiquitin-protein ligase RAD18; EC 6.3.2.-; hHR 18; hHR18; hRAD 18; hRAD18; MGC156682; Postreplication repair E3 ubiquitin-protein ligase RAD18; Postreplication repair protein hRAD18p; Postreplication repair protein RAD18; RAD 18; RAD18; RAD18 homolog (S. cerevisiae); RAD18 homolog; RAD18 S. cerevisiae homolog; RAD18 S. cerevisiae homolog of; RAD18 transcript variant; RAD18_HUMAN; Rad18sc; Radiation sensitivity protein 18; RING finger protein 73; RNF 73; RNF73; Structural maintenance of chromosomes protein 6; YCR066W; YCR66W;
抗原和靶标
- Q9NS91 RAD18_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDSLAESRWPPGLAVMKTIDDLLRCGICFEYFNIAMIIPQCSHNYCSLCIRKFLSYKTQCPTCCVTVTEPDLKNNRILDELVKSLNFARNHLLQFALESPAKSPASSSSKNLAVKVYTPVASRQSLKQGSRLMDNFLIREMSGSTSELLIKENKSKFSPQKEASPAAKTKETRSVEEIAPDPSEAKRPEPPSTSTLKQVTKVDCPVCGVNIPESHINKHLDSCLSREEKKESLRSSVHKRKPLPKTVYNLLSDRDLKKKLKEHGLSIQGNKQQLIKRHQEFVHMYNAQCDALHPKSAAEIVREIENIEKTRMRLEASKLNESVMVFTKDQTEKEIDEIHSKYRKKHKSEFQLLVDQARKGYKKIAGMSQKTVTITKEDESTEKLSSVCMGQEDNMTSVTNHFSQSKLDSPEELEPDREEDSSSCIDIQEVLSSSESDSCNSSSSDIIRDLLEEEEAWEASHKNDLQDTEISPRQNRRTRAAESAEIEPRNKRNRN
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9NS91 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| K17 | Ubiquitination | Uniprot | |
| K52 | Ubiquitination | Uniprot | |
| S55 | Phosphorylation | Uniprot | |
| Y56 | Phosphorylation | Uniprot | |
| K57 | Ubiquitination | Uniprot | |
| T66 | Phosphorylation | Uniprot | |
| K73 | Ubiquitination | Uniprot | |
| K83 | Ubiquitination | Uniprot | |
| S99 | Phosphorylation | P24941 (CDK2) | Uniprot |
| K102 | Sumoylation | Uniprot | |
| K102 | Ubiquitination | Uniprot | |
| S103 | Phosphorylation | Uniprot | |
| S106 | Phosphorylation | Uniprot | |
| S107 | Phosphorylation | Uniprot | |
| S108 | Phosphorylation | Uniprot | |
| S109 | Phosphorylation | Uniprot | |
| K110 | Sumoylation | Uniprot | |
| K110 | Ubiquitination | Uniprot | |
| K115 | Sumoylation | Uniprot | |
| K115 | Ubiquitination | Uniprot | |
| T118 | Phosphorylation | Uniprot | |
| S122 | Phosphorylation | Uniprot | |
| S125 | Phosphorylation | Uniprot | |
| K127 | Sumoylation | Uniprot | |
| K127 | Ubiquitination | Uniprot | |
| S142 | Phosphorylation | Uniprot | |
| S144 | Phosphorylation | Uniprot | |
| K151 | Sumoylation | Uniprot | |
| K151 | Ubiquitination | Uniprot | |
| K154 | Ubiquitination | Uniprot | |
| S155 | Phosphorylation | Uniprot | |
| K156 | Ubiquitination | Uniprot | |
| S158 | Phosphorylation | Uniprot | |
| K161 | Ubiquitination | Uniprot | |
| S164 | Phosphorylation | Uniprot | |
| K168 | Ubiquitination | Uniprot | |
| K170 | Ubiquitination | Uniprot | |
| S174 | Phosphorylation | Uniprot | |
| K186 | Sumoylation | Uniprot | |
| K186 | Ubiquitination | Uniprot | |
| K197 | Sumoylation | Uniprot | |
| K197 | Ubiquitination | Uniprot | |
| K201 | Sumoylation | Uniprot | |
| K201 | Ubiquitination | Uniprot | |
| K218 | Ubiquitination | Uniprot | |
| K229 | Sumoylation | Uniprot | |
| K245 | Ubiquitination | Uniprot | |
| R254 | Methylation | Uniprot | |
| K257 | Sumoylation | Uniprot | |
| K257 | Ubiquitination | Uniprot | |
| K259 | Ubiquitination | Uniprot | |
| K261 | Sumoylation | Uniprot | |
| K261 | Ubiquitination | Uniprot | |
| K271 | Ubiquitination | Uniprot | |
| K276 | Ubiquitination | Uniprot | |
| K309 | Ubiquitination | Uniprot | |
| K318 | Sumoylation | Uniprot | |
| K318 | Ubiquitination | Uniprot | |
| S322 | Phosphorylation | Uniprot | |
| T327 | Phosphorylation | Uniprot | |
| K328 | Sumoylation | Uniprot | |
| K328 | Ubiquitination | Uniprot | |
| K333 | Ubiquitination | Uniprot | |
| K341 | Ubiquitination | Uniprot | |
| K347 | Sumoylation | Uniprot | |
| K347 | Ubiquitination | Uniprot | |
| K363 | Ubiquitination | Uniprot | |
| S368 | Phosphorylation | Uniprot | |
| K370 | Acetylation | Uniprot | |
| K370 | Sumoylation | Uniprot | |
| K370 | Ubiquitination | Uniprot | |
| K376 | Sumoylation | Uniprot | |
| K376 | Ubiquitination | Uniprot | |
| K383 | Ubiquitination | Uniprot | |
| S385 | Phosphorylation | Uniprot | |
| S386 | Phosphorylation | Uniprot | |
| S403 | Phosphorylation | Q13315 (ATM) | Uniprot |
| S409 | Phosphorylation | Uniprot | |
| S434 | Phosphorylation | O00311 (CDC7) | Uniprot |
| K462 | Ubiquitination | Uniprot | |
| T468 | Phosphorylation | Uniprot | |
| S471 | Phosphorylation | Uniprot | |
| S483 | Phosphorylation | Uniprot |
研究背景
E3 ubiquitin-protein ligase involved in postreplication repair of UV-damaged DNA. Postreplication repair functions in gap-filling of a daughter strand on replication of damaged DNA. Associates to the E2 ubiquitin conjugating enzyme UBE2B to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. Has ssDNA binding activity.
Nucleus. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome.
Note: Associates with chromatin (PubMed:25931565). Colocalizes with SLF1 in the nucleus and to centrosomes (PubMed:15632077). Relocalizes with SLF1 to nuclear foci in response to DNA damage (PubMed:22036607). Accumulates with the SLF1-SLF2 and SMC5-SMC6 complexes at replication-coupled DNA interstrand repair and DNA double-strand breaks (DSBs) sites on chromatin in a ubiquitin-dependent manner (PubMed:25931565).
Homodimer. Interacts with UBE2A and UBE2B, one homodimer binding one molecule of UBE2B. Interacts with SHPRH. Interacts with HLTF. Interacts with SPRTN; leading to enhance chromatin association of RAD18 and RAD18-mediated PCNA ubiquitination and translesion DNA synthesis. Interacts (via C-terminus and phosphorylated form) with SLF1 (via BRCT domains); this interaction is required for efficient repair of UV-induced DNA damage. Interacts with SLF2. Interacts with SMC5; this interaction is increased in a SLF1 or SLF2-dependent manner.
Belongs to the RAD18 family.
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