Phospho-VAV2 (Tyr142) Antibody - #AF4446

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产品: 磷酸化 VAV2 (Tyr142) 抗体
货号: AF4446
描述: Rabbit polyclonal antibody to Phospho-VAV2 (Tyr142)
应用: WB
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Horse, Chicken, Xenopus
分子量: 101kDa; 101kD(Calculated).
蛋白号: P52735
RRID: AB_2844631

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 50ul RMB¥ 1500 现货
 100ul RMB¥ 2800 现货
 200ul RMB¥ 3800 现货

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MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(92%), Zebrafish(92%), Horse(100%), Chicken(100%), Xenopus(92%)
克隆:
Polyclonal
特异性:
Phospho-VAV2 (Tyr142) Antibody detects endogenous levels of VAV2 only when phosphorylated at Tyr142.
RRID:
AB_2844631
引用格式: Affinity Biosciences Cat# AF4446, RRID:AB_2844631.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Guanine nucleotide exchange factor VAV2; Oncogene VAV2; Protein vav 2; VAV 2; Vav 2 oncogene; VAV-2; VAV2; VAV2 oncogene; VAV2_HUMAN;

抗原和靶标

免疫原:
Uniprot:

P52735(VAV2_HUMAN) >>Visit HPA database.

基因/基因ID:
VAV2(7410)
表达:
P52735 VAV2_HUMAN:

Widely expressed.

序列:
MEQWRQCGRWLIDCKVLPPNHRVVWPSAVVFDLAQALRDGVLLCQLLHNLSPGSIDLKDINFRPQMSQFLCLKNIRTFLKVCHDKFGLRNSELFDPFDLFDVRDFGKVISAVSRLSLHSIAQNKGIRPFPSEETTENDDDVYRSLEELADEHDLGEDIYDCVPCEDGGDDIYEDIIKVEVQQPMIRYMQKMGMTEDDKRNCCLLEIQETEAKYYRTLEDIEKNYMSPLRLVLSPADMAAVFINLEDLIKVHHSFLRAIDVSVMVGGSTLAKVFLDFKERLLIYGEYCSHMEHAQNTLNQLLASREDFRQKVEECTLKVQDGKFKLQDLLVVPMQRVLKYHLLLKELLSHSAERPERQQLKEALEAMQDLAMYINEVKRDKETLRKISEFQSSIENLQVKLEEFGRPKIDGELKVRSIVNHTKQDRYLFLFDKVVIVCKRKGYSYELKEIIELLFHKMTDDPMNNKDVKKSHGKMWSYGFYLIHLQGKQGFQFFCKTEDMKRKWMEQFEMAMSNIKPDKANANHHSFQMYTFDKTTNCKACKMFLRGTFYQGYMCTKCGVGAHKECLEVIPPCKFTSPADLDASGAGPGPKMVAMQNYHGNPAPPGKPVLTFQTGDVLELLRGDPESPWWEGRLVQTRKSGYFPSSSVKPCPVDGRPPISRPPSREIDYTAYPWFAGNMERQQTDNLLKSHASGTYLIRERPAEAERFAISIKFNDEVKHIKVVEKDNWIHITEAKKFDSLLELVEYYQCHSLKESFKQLDTTLKYPYKSRERSASRASSRSPASCASYNFSFLSPQGLSFASQGPSAPFWSVFTPRVIGTAVARYNFAARDMRELSLREGDVVRIYSRIGGDQGWWKGETNGRIGWFPSTYVEEEGIQ

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Horse
100
Chicken
100
Pig
92
Xenopus
92
Zebrafish
92
Bovine
57
Sheep
57
Dog
57
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P52735 作为底物

Site PTM Type Enzyme
S91 Phosphorylation
K124 Ubiquitination
Y142 Phosphorylation P06239 (LCK) , P00533 (EGFR)
Y159 Phosphorylation P06239 (LCK) , P00533 (EGFR)
Y172 Phosphorylation P06239 (LCK) , P00533 (EGFR)
K212 Ubiquitination
K385 Ubiquitination
K399 Ubiquitination
K407 Ubiquitination
K413 Ubiquitination
Y426 Phosphorylation
K515 Acetylation
K573 Ubiquitination
T575 Phosphorylation
S576 Phosphorylation
S583 Phosphorylation
S626 Phosphorylation
S639 Phosphorylation
S663 Phosphorylation
S692 Phosphorylation
K718 Ubiquitination
K757 Ubiquitination
S778 Phosphorylation
S779 Phosphorylation
S781 Phosphorylation
S836 Phosphorylation

研究背景

功能:

Guanine nucleotide exchange factor for the Rho family of Ras-related GTPases. Plays an important role in angiogenesis. Its recruitment by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly (By similarity).

翻译修饰:

Phosphorylated on tyrosine residues in response to FGR activation.

组织特异性:

Widely expressed.

亚基结构:

Interacts (via SH2 domains) with the phosphorylated form of EPHA2. Interacts with SSX2IP (By similarity). Interacts with NEK3 and PRLR and this interaction is prolactin-dependent.

研究领域

· Cellular Processes > Cellular community - eukaryotes > Focal adhesion.   (View pathway)

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.   (View pathway)

· Environmental Information Processing > Signal transduction > Rap1 signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > cAMP signaling pathway.   (View pathway)

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Organismal Systems > Immune system > Chemokine signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Natural killer cell mediated cytotoxicity.   (View pathway)

· Organismal Systems > Immune system > T cell receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > B cell receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Fc epsilon RI signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Fc gamma R-mediated phagocytosis.   (View pathway)

· Organismal Systems > Immune system > Leukocyte transendothelial migration.   (View pathway)

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