产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF4449, RRID:AB_2844633.
展开/折叠
Blepharophimosis; Blepharophimosis epicanthus inversus and ptosis 1; Blepharophimosis epicanthus inversus and ptosis; BPES 1; BPES; BPES1; Epicanthus inversus and ptosis 1; Forkhead box L2; Forkhead box protein L2; Forkhead transcription factor FOXL2; FOX L2; FOXL 2; FOXL2; FOXL2_HUMAN; PFRK; PINTO; PITUITARY FORKHEAD FACTOR; POF 3; POF3;
抗原和靶标
In addition to its expression in the developing eyelid, it is transcribed very early in somatic cells of the developing gonad (before sex determination) and its expression persists in the follicular cells of the adult ovary.
- P58012 FOXL2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MMASYPEPEDAAGALLAPETGRTVKEPEGPPPSPGKGGGGGGGTAPEKPDPAQKPPYSYVALIAMAIRESAEKRLTLSGIYQYIIAKFPFYEKNKKGWQNSIRHNLSLNECFIKVPREGGGERKGNYWTLDPACEDMFEKGNYRRRRRMKRPFRPPPAHFQPGKGLFGAGGAAGGCGVAGAGADGYGYLAPPKYLQSGFLNNSWPLPQPPSPMPYASCQMAAAAAAAAAAAAAAGPGSPGAAAVVKGLAGPAASYGPYTRVQSMALPPGVVNSYNGLGGPPAAPPPPPHPHPHPHAHHLHAAAAPPPAPPHHGAAAPPPGQLSPASPATAAPPAPAPTSAPGLQFACARQPELAMMHCSYWDHDSKTGALHSRLDL
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P58012 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S4 | Phosphorylation | Uniprot | |
| Y5 | Phosphorylation | Uniprot | |
| K25 | Sumoylation | Uniprot | |
| S33 | Phosphorylation | P49841 (GSK3B) | Uniprot |
| Y81 | Phosphorylation | Uniprot | |
| Y83 | Phosphorylation | Uniprot | |
| Y91 | Phosphorylation | Uniprot | |
| K96 | Acetylation | Uniprot | |
| K96 | Ubiquitination | Uniprot | |
| S101 | Phosphorylation | Uniprot | |
| S107 | Phosphorylation | Uniprot | |
| K114 | Sumoylation | Uniprot | |
| Y127 | Phosphorylation | Uniprot | |
| K140 | Acetylation | Uniprot | |
| Y186 | Phosphorylation | Uniprot | |
| S211 | Phosphorylation | Uniprot | |
| Y215 | Phosphorylation | Uniprot | |
| S238 | Phosphorylation | Uniprot | |
| Y258 | Phosphorylation | Uniprot | |
| S263 | Phosphorylation | Q13153 (PAK1) | Uniprot |
| S323 | Phosphorylation | Uniprot | |
| S326 | Phosphorylation | Uniprot | |
| T329 | Phosphorylation | Uniprot | |
| K366 | Acetylation | Uniprot |
研究背景
Transcriptional regulator. Critical factor essential for ovary differentiation and maintenance, and repression of the genetic program for somatic testis determination. Prevents trans-differentiation of ovary to testis through transcriptional repression of the Sertoli cell-promoting gene SOX9 (By similarity). Has apoptotic activity in ovarian cells. Suppresses ESR1-mediated transcription of PTGS2/COX2 stimulated by tamoxifen (By similarity). Is a regulator of CYP19 expression (By similarity). Participates in SMAD3-dependent transcription of FST via the intronic SMAD-binding element (By similarity). Is a transcriptional repressor of STAR. Activates SIRT1 transcription under cellular stress conditions. Activates transcription of OSR2.
Sumoylated with SUMO1; sumoylation is required for transcriptional repression activity.
Nucleus.
In addition to its expression in the developing eyelid, it is transcribed very early in somatic cells of the developing gonad (before sex determination) and its expression persists in the follicular cells of the adult ovary.
Interacts with ESR1 (By similarity). Interacts with SMAD3 (By similarity). Interacts with DDX20. Interacts with UBE2I/UBC9.
限制条款
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