| 产品: | 磷酸化 RapGEF1 (Thr1071) 抗体 |
| 货号: | AF4469 |
| 描述: | Rabbit polyclonal antibody to Phospho-RapGEF1 (Thr1071) |
| 应用: | WB IHC |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 121kDa; 121kD(Calculated). |
| 蛋白号: | Q13905 |
| RRID: | AB_2844637 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF4469, RRID:AB_2844637.
展开/折叠
C3G; C3G protein; CRK SH3 binding GNRP; CRK SH3-binding GNRP; DKFZp781P1719; GRF 2; GRF2; Guanine nucleotide releasing factor 2 (specific for crk proto oncogene); Guanine nucleotide releasing factor 2; Guanine nucleotide-releasing factor 2; OTTHUMP00000064558; Protein C3G; Rap guanine nucleotide exchange factor (GEF) 1; Rap guanine nucleotide exchange factor 1; RAPGEF 1; RAPGEF1; RPGF1_HUMAN;
抗原和靶标
Ubiquitously expressed in adult and fetus. Expression is high in adult skeletal muscle and placenta and in fetal brain and heart. Low levels of expression in adult and fetal liver.
- Q13905 RPGF1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDTDSQRSHLSSFTMKLMDKFHSPKIKRTPSKKGKPAEVSVKIPEKPVNKEATDRFLPEGYPLPLDLEQQAVEFMSTSAVASRSQRQKNLSWLEEKEKEVVSALRYFKTIVDKMAIDKKVLEMLPGSASKVLEAILPLVQNDPRIQHSSALSSCYSRVYQSLANLIRWSDQVMLEGVNSEDKEMVTTVKGVIKAVLDGVKELVRLTIEKQGRPSPTSPVKPSSPASKPDGPAELPLTDREVEILNKTTGMSQSTELLPDATDEEVAPPKPPLPGIRVVDNSPPPALPPKKRQSAPSPTRVAVVAPMSRATSGSSLPVGINRQDFDVDCYAQRRLSGGSHSYGGESPRLSPCSSIGKLSKSDEQLSSLDRDSGQCSRNTSCETLDHYDPDYEFLQQDLSNADQIPQQTAWNLSPLPESLGESGSPFLGPPFQLPLGGHPQPDGPLAPGQQTDTPPALPEKKRRSAASQTADGSGCRVSYERHPSQYDNISGEDLQSTAPIPSVPYAPFAAILPFQHGGSSAPVEFVGDFTAPESTGDPEKPPPLPEKKNKHMLAYMQLLEDYSEPQPSMFYQTPQNEHIYQQKNKLLMEVYGFSDSFSGVDSVQELAPPPALPPKQRQLEPPAGKDGHPRDPSAVSGVPGKDSRDGSERAPKSPDALESAQSEEEVDELSLIDHNEIMSRLTLKQEGDDGPDVRGGSGDILLVHATETDRKDLVLYCEAFLTTYRTFISPEELIKKLQYRYEKFSPFADTFKKRVSKNTFFVLVRVVDELCLVELTEEILKLLMELVFRLVCNGELSLARVLRKNILDKVDQKKLLRCATSSQPLAARGVAARPGTLHDFHSHEIAEQLTLLDAELFYKIEIPEVLLWAKEQNEEKSPNLTQFTEHFNNMSYWVRSIIMLQEKAQDRERLLLKFIKIMKHLRKLNNFNSYLAILSALDSAPIRRLEWQKQTSEGLAEYCTLIDSSSSFRAYRAALSEVEPPCIPYLGLILQDLTFVHLGNPDYIDGKVNFSKRWQQFNILDSMRCFQQAHYDMRRNDDIINFFNDFSDHLAEEALWELSLKIKPRNITRRKTDREEKT
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q13905 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S12 | Phosphorylation | Uniprot | |
| S23 | Phosphorylation | Uniprot | |
| Y61 | Phosphorylation | Uniprot | |
| S76 | Phosphorylation | Uniprot | |
| T109 | Phosphorylation | Uniprot | |
| K200 | Ubiquitination | Uniprot | |
| S214 | Phosphorylation | Uniprot | |
| S217 | Phosphorylation | Uniprot | |
| S222 | Phosphorylation | Uniprot | |
| S223 | Phosphorylation | Uniprot | |
| S253 | Phosphorylation | Uniprot | |
| T261 | Phosphorylation | Uniprot | |
| S281 | Phosphorylation | Uniprot | |
| S293 | Phosphorylation | Uniprot | |
| S296 | Phosphorylation | Uniprot | |
| S307 | Phosphorylation | Uniprot | |
| T310 | Phosphorylation | Uniprot | |
| S311 | Phosphorylation | Uniprot | |
| S313 | Phosphorylation | Uniprot | |
| S314 | Phosphorylation | Uniprot | |
| Y329 | Phosphorylation | Uniprot | |
| S335 | Phosphorylation | Uniprot | |
| S338 | Phosphorylation | Uniprot | |
| Y341 | Phosphorylation | Uniprot | |
| S345 | Phosphorylation | Uniprot | |
| S349 | Phosphorylation | Uniprot | |
| S352 | Phosphorylation | Uniprot | |
| S353 | Phosphorylation | Uniprot | |
| S358 | Phosphorylation | Uniprot | |
| S360 | Phosphorylation | Uniprot | |
| S366 | Phosphorylation | Uniprot | |
| S371 | Phosphorylation | Uniprot | |
| Y390 | Phosphorylation | Uniprot | |
| S423 | Phosphorylation | Uniprot | |
| S463 | Phosphorylation | Uniprot | |
| S466 | Phosphorylation | Uniprot | |
| Y504 | Phosphorylation | P00519 (ABL1) , P12931 (SRC) , P08631 (HCK) | Uniprot |
| Y561 | Phosphorylation | Uniprot | |
| Y570 | Phosphorylation | Uniprot | |
| T572 | Phosphorylation | Uniprot | |
| Y579 | Phosphorylation | Uniprot | |
| S652 | Phosphorylation | Uniprot | |
| K683 | Ubiquitination | Uniprot | |
| Y715 | Phosphorylation | Uniprot | |
| Y723 | Phosphorylation | Uniprot | |
| K808 | Ubiquitination | Uniprot | |
| T819 | Phosphorylation | Uniprot | |
| S820 | Phosphorylation | Uniprot | |
| K869 | Ubiquitination | Uniprot | |
| T950 | Phosphorylation | Uniprot | |
| S951 | Phosphorylation | Uniprot | |
| S965 | Phosphorylation | Uniprot | |
| S966 | Phosphorylation | Uniprot | |
| T1067 | Phosphorylation | Uniprot | |
| T1071 | Phosphorylation | Uniprot |
研究背景
Guanine nucleotide-releasing protein that binds to SH3 domain of CRK and GRB2/ASH. Transduces signals from CRK to activate RAS. Plays a role in the establishment of basal endothelial barrier function. Plays a role in nerve growth factor (NGF)-induced sustained activation of Rap1 and neurite outgrowth.
Phosphorylation at Tyr-504 enhances activity as Rap guanine nucleotide exchange factor.
Early endosome.
Ubiquitously expressed in adult and fetus. Expression is high in adult skeletal muscle and placenta and in fetal brain and heart. Low levels of expression in adult and fetal liver.
Interacts with CRK via its SH3-binding sites.
研究领域
· Cellular Processes > Cellular community - eukaryotes > Focal adhesion. (View pathway)
· Environmental Information Processing > Signal transduction > Rap1 signaling pathway. (View pathway)
· Human Diseases > Cancers: Specific types > Renal cell carcinoma. (View pathway)
· Organismal Systems > Nervous system > Neurotrophin signaling pathway. (View pathway)
· Organismal Systems > Endocrine system > Insulin signaling pathway. (View pathway)
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