| 产品: | 磷酸化 PEA15 (Ser116) 抗体 |
| 货号: | AF4474 |
| 描述: | Rabbit polyclonal antibody to Phospho-PEA15 (Ser116) |
| 应用: | WB |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus |
| 分子量: | 15kDa; 15kD(Calculated). |
| 蛋白号: | Q15121 |
| RRID: | AB_2844639 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF4474, RRID:AB_2844639.
展开/折叠
15 kDa phosphoprotein enriched in astrocytes; Astrocytic phosphoprotein PEA 15; Astrocytic phosphoprotein PEA-15; Astrocytic phosphoprotein PEA15; HMAT 1; HMAT1; Homolog of mouse MAT 1 oncogene; Homolog of mouse MAT1 oncogene; HUMMAT 1H; HUMMAT1H; MAT 1; MAT 1H; MAT1; MAT1H; PEA 15; Pea15; PEA15 protein; PEA15_HUMAN; PED; Phosphoprotein enriched in astrocytes 15; Phosphoprotein enriched in astrocytes 15kD; Phosphoprotein enriched in diabetes;
抗原和靶标
Ubiquitously expressed. Most abundant in tissues such as heart, brain, muscle and adipose tissue which utilize glucose as an energy source. Lower expression in glucose-producing tissues. Higher levels of expression are found in tissues from individuals with type 2 diabetes than in controls.
- Q15121 PEA15_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAEYGTLLQDLTNNITLEDLEQLKSACKEDIPSEKSEEITTGSAWFSFLESHNKLDKDNLSYIEHIFEISRRPDLLTMVVDYRTRVLKISEEDELDTKLTRIPSAKKYKDIIRQPSEEEIIKLAPPPKKA
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q15121 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y4 | Phosphorylation | Uniprot | |
| T6 | Phosphorylation | Uniprot | |
| K24 | Ubiquitination | Uniprot | |
| S25 | Phosphorylation | Uniprot | |
| C27 | S-Nitrosylation | Uniprot | |
| K28 | Ubiquitination | Uniprot | |
| S43 | Phosphorylation | Uniprot | |
| K54 | Ubiquitination | Uniprot | |
| S61 | Phosphorylation | Uniprot | |
| S90 | Phosphorylation | Uniprot | |
| T97 | Phosphorylation | Uniprot | |
| K98 | Ubiquitination | Uniprot | |
| T100 | Phosphorylation | Uniprot | |
| S104 | Phosphorylation | P17252 (PRKCA) | Uniprot |
| Y108 | Phosphorylation | Uniprot | |
| K109 | Methylation | Uniprot | |
| S116 | Phosphorylation | P31749 (AKT1) , Q9UQM7 (CAMK2A) , P54646 (PRKAA2) | Uniprot |
研究背景
Blocks Ras-mediated inhibition of integrin activation and modulates the ERK MAP kinase cascade. Inhibits RPS6KA3 activities by retaining it in the cytoplasm (By similarity). Inhibits both TNFRSF6- and TNFRSF1A-mediated CASP8 activity and apoptosis. Regulates glucose transport by controlling both the content of SLC2A1 glucose transporters on the plasma membrane and the insulin-dependent trafficking of SLC2A4 from the cell interior to the surface.
Phosphorylated by protein kinase C and calcium-calmodulin-dependent protein kinase. These phosphorylation events are modulated by neurotransmitters or hormones.
Cytoplasm.
Note: Associated with microtubules.
Ubiquitously expressed. Most abundant in tissues such as heart, brain, muscle and adipose tissue which utilize glucose as an energy source. Lower expression in glucose-producing tissues. Higher levels of expression are found in tissues from individuals with type 2 diabetes than in controls.
Binds RPS6KA3, MAPK3 and MAPK1. Transient interaction with PLD1 and PLD2 (By similarity). Interacts with CASP8 and FADD.
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