Phospho-LIMK2 (Ser283) Antibody - #AF3346
(1)
(18)
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF3346, RRID:AB_2834761.
展开/折叠
LIM containing protein kinase 2b; LIM domain kinase 2; LIM domain kinase 2b; LIM kinase 2b; LIM motif containing protein kinase 2b; LIMK 2b; LIMK-2; Limk2; LIMK2 b; LIMK2_HUMAN; LIMK2b;
抗原和靶标
Highest expression in the placenta; moderate level in liver, lung, kidney, and pancreas. LIMK2a is found to be more abundant then LIMK2b in liver, colon, stomach, and spleen, while in brain, kidney, and placenta LIMK2b is the dominant form. In adult lung, both LIMK2a and LIMK2b is nearly equally observed.
- P53671 LIMK2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSALAGEDVWRCPGCGDHIAPSQIWYRTVNETWHGSCFRCSECQDSLTNWYYEKDGKLYCPKDYWGKFGEFCHGCSLLMTGPFMVAGEFKYHPECFACMSCKVIIEDGDAYALVQHATLYCGKCHNEVVLAPMFERLSTESVQEQLPYSVTLISMPATTEGRRGFSVSVESACSNYATTVQVKEVNRMHISPNNRNAIHPGDRILEINGTPVRTLRVEEVEDAISQTSQTLQLLIEHDPVSQRLDQLRLEARLAPHMQNAGHPHALSTLDTKENLEGTLRRRSLRRSNSISKSPGPSSPKEPLLFSRDISRSESLRCSSSYSQQIFRPCDLIHGEVLGKGFFGQAIKVTHKATGKVMVMKELIRCDEETQKTFLTEVKVMRSLDHPNVLKFIGVLYKDKKLNLLTEYIEGGTLKDFLRSMDPFPWQQKVRFAKGIASGMAYLHSMCIIHRDLNSHNCLIKLDKTVVVADFGLSRLIVEERKRAPMEKATTKKRTLRKNDRKKRYTVVGNPYWMAPEMLNGKSYDETVDIFSFGIVLCEIIGQVYADPDCLPRTLDFGLNVKLFWEKFVPTDCPPAFFPLAAICCRLEPESRPAFSKLEDSFEALSLYLGELGIPLPAELEELDHTVSMQYGLTRDSPP
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P53671 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K57 | Ubiquitination | Uniprot | |
| K62 | Ubiquitination | Uniprot | |
| S149 | Phosphorylation | Uniprot | |
| T151 | Phosphorylation | Uniprot | |
| S166 | Phosphorylation | Uniprot | |
| S168 | Phosphorylation | Uniprot | |
| T179 | Phosphorylation | Uniprot | |
| S191 | Phosphorylation | Uniprot | |
| T210 | Phosphorylation | Uniprot | |
| K272 | Ubiquitination | Uniprot | |
| T278 | Phosphorylation | Uniprot | |
| S283 | Phosphorylation | O14965 (AURKA) , Q05655 (PRKCD) | Uniprot |
| S287 | Phosphorylation | Uniprot | |
| S289 | Phosphorylation | Uniprot | |
| S291 | Phosphorylation | Uniprot | |
| K292 | Ubiquitination | Uniprot | |
| S293 | Phosphorylation | Uniprot | |
| S297 | Phosphorylation | Uniprot | |
| S298 | Phosphorylation | Uniprot | |
| K300 | Ubiquitination | Uniprot | |
| S310 | Phosphorylation | Uniprot | |
| S318 | Phosphorylation | Uniprot | |
| K347 | Ubiquitination | Uniprot | |
| K355 | Acetylation | Uniprot | |
| K355 | Ubiquitination | Uniprot | |
| K360 | Ubiquitination | Uniprot | |
| K371 | Ubiquitination | Uniprot | |
| K378 | Ubiquitination | Uniprot | |
| K414 | Ubiquitination | Uniprot | |
| K428 | Ubiquitination | Uniprot | |
| K487 | Ubiquitination | Uniprot | |
| T494 | Phosphorylation | O14965 (AURKA) | Uniprot |
| T505 | Phosphorylation | Q5VT25 (CDC42BPA) , O14965 (AURKA) , Q13464 (ROCK1) , O75116 (ROCK2) | Uniprot |
研究背景
Serine/threonine-protein kinase that plays an essential role in the regulation of actin filament dynamics. Acts downstream of several Rho family GTPase signal transduction pathways. Involved in astral microtubule organization and mitotic spindle orientation during early stage of mitosis by mediating phosphorylation of TPPP. Displays serine/threonine-specific phosphorylation of myelin basic protein and histone (MBP) in vitro.
Phosphorylated on serine and/or threonine residues by ROCK1.
Cytoplasm. Nucleus. Cytoplasm>Cytoskeleton>Spindle.
Cytoplasm. Nucleus.
Note: Isoform LIMK2a is distributed in the cytoplasm and the nucleus.
Cytoplasm. Nucleus.
Note: Isoform LIMK2b occurs mainly in the cytoplasm and is scarcely translocated to the nucleus.
Highest expression in the placenta; moderate level in liver, lung, kidney, and pancreas. LIMK2a is found to be more abundant then LIMK2b in liver, colon, stomach, and spleen, while in brain, kidney, and placenta LIMK2b is the dominant form. In adult lung, both LIMK2a and LIMK2b is nearly equally observed.
Binds ROCK1 and MARF1 (Ref.9,. Interacts with NISCH (By similarity).
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
研究领域
· Cellular Processes > Cell motility > Regulation of actin cytoskeleton. (View pathway)
· Organismal Systems > Development > Axon guidance. (View pathway)
· Organismal Systems > Immune system > Fc gamma R-mediated phagocytosis. (View pathway)
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