Phospho-Ataxin 1 (Ser775) Antibody - #AF3347
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF3347, RRID:AB_2834762.
展开/折叠
alternative ataxin1; Ataxin-1; ATX1; ATX1_HUMAN; Atxn1; D6S504E; OTTHUMP00000016065; SCA1; Spinocerebellar ataxia type 1 protein;
抗原和靶标
- P54253 ATX1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MKSNQERSNECLPPKKREIPATSRSSEEKAPTLPSDNHRVEGTAWLPGNPGGRGHGGGRHGPAGTSVELGLQQGIGLHKALSTGLDYSPPSAPRSVPVATTLPAAYATPQPGTPVSPVQYAHLPHTFQFIGSSQYSGTYASFIPSQLIPPTANPVTSAVASAAGATTPSQRSQLEAYSTLLANMGSLSQTPGHKAEQQQQQQQQQQQQHQHQQQQQQQQQQQQQQHLSRAPGLITPGSPPPAQQNQYVHISSSPQNTGRTASPPAIPVHLHPHQTMIPHTLTLGPPSQVVMQYADSGSHFVPREATKKAESSRLQQAIQAKEVLNGEMEKSRRYGAPSSADLGLGKAGGKSVPHPYESRHVVVHPSPSDYSSRDPSGVRASVMVLPNSNTPAADLEVQQATHREASPSTLNDKSGLHLGKPGHRSYALSPHTVIQTTHSASEPLPVGLPATAFYAGTQPPVIGYLSGQQQAITYAGSLPQHLVIPGTQPLLIPVGSTDMEASGAAPAIVTSSPQFAAVPHTFVTTALPKSENFNPEALVTQAAYPAMVQAQIHLPVVQSVASPAAAPPTLPPYFMKGSIIQLANGELKKVEDLKTEDFIQSAEISNDLKIDSSTVERIEDSHSPGVAVIQFAVGEHRAQVSVEVLVEYPFFVFGQGWSSCCPERTSQLFDLPCSKLSVGDVCISLTLKNLKNGSVKKGQPVDPASVLLKHSKADGLAGSRHRYAEQENGINQGSAQMLSENGELKFPEKMGLPAAPFLTKIEPSKPAATRKRRWSAPESRKLEKSEDEPPLTLPKPSLIPQEVKICIEGRSNVGK
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P54253 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K16 | Sumoylation | Uniprot | |
| R53 | Methylation | Uniprot | |
| S82 | Phosphorylation | Uniprot | |
| S88 | Phosphorylation | Uniprot | |
| S91 | Phosphorylation | Uniprot | |
| K194 | Sumoylation | Uniprot | |
| T235 | Phosphorylation | Uniprot | |
| S238 | Phosphorylation | Uniprot | |
| Y247 | Phosphorylation | Uniprot | |
| S253 | Phosphorylation | Uniprot | |
| T257 | Phosphorylation | Uniprot | |
| S312 | Phosphorylation | Uniprot | |
| S366 | Phosphorylation | Uniprot | |
| S406 | Phosphorylation | Uniprot | |
| S408 | Phosphorylation | Uniprot | |
| K609 | Sumoylation | Uniprot | |
| K696 | Sumoylation | Uniprot | |
| S711 | Phosphorylation | Uniprot | |
| S739 | Phosphorylation | Uniprot | |
| K745 | Sumoylation | Uniprot | |
| T769 | Phosphorylation | Uniprot | |
| S775 | Phosphorylation | P31749 (AKT1) , O75582 (RPS6KA5) , P17612 (PRKACA) | Uniprot |
| S811 | Phosphorylation | Uniprot |
研究背景
Chromatin-binding factor that repress Notch signaling in the absence of Notch intracellular domain by acting as a CBF1 corepressor. Binds to the HEY promoter and might assist, along with NCOR2, RBPJ-mediated repression. Binds RNA in vitro. May be involved in RNA metabolism. In concert with CIC and ATXN1L, involved in brain development (By similarity).
Ubiquitinated by UBE3A, leading to its degradation by the proteasome. The presence of expanded poly-Gln repeats in spinocerebellar ataxia 1 (SCA1) patients impairs ubiquitination and degradation, leading to accumulation of ATXN1 in neurons and subsequent toxicity.
Phosphorylation at Ser-775 increases the pathogenicity of proteins with an expanded polyglutamine tract.
Sumoylation is dependent on nuclear localization and phosphorylation at Ser-775. It is reduced in the presence of an expanded polyglutamine tract.
Cytoplasm. Nucleus.
Note: Colocalizes with USP7 in the nucleus.
Widely expressed throughout the body.
Homooligomer. Interacts with CIC (By similarity). Interacts with ANP32A, PQBP1, UBQLN4, ATXN1L and USP7. Directly interacts with RBPJ; this interaction is disrupted in the presence of Notch intracellular domain. Competes with ATXN1L for RBPJ-binding. Found in a complex with CIC and ATXN1L (By similarity).
The AXH domain is required for interaction with CIC.
Belongs to the ATXN1 family.
限制条款
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