Phospho-4E-BP1 (Ser65/Thr70) Antibody - #AF2308
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF2308, RRID:AB_2845322.
展开/折叠
4E-BP1; 4EBP1; 4EBP1_HUMAN; BP 1; eIF4E binding protein 1; eIF4E-binding protein 1; Eif4ebp1; Eukaryotic translation initiation factor 4E-binding protein 1; PHAS-I; PHASI; Phosphorylated heat- and acid-stable protein regulated by insulin 1;
抗原和靶标
- Q13541 4EBP1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSGGSSCSQTPSRAIPATRRVVLGDGVQLPPGDYSTTPGGTLFSTTPGGTRIIYDRKFLMECRNSPVTKTPPRDLPTIPGVTSPSSDEPPMEASQSHLRNSPEDKRAGGEESQFEMDI
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q13541 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Acetylation | Uniprot | |
| S2 | Phosphorylation | Uniprot | |
| S5 | Phosphorylation | Uniprot | |
| S6 | Phosphorylation | Uniprot | |
| S8 | Phosphorylation | Q13315 (ATM) | Uniprot |
| T10 | Phosphorylation | Uniprot | |
| S12 | Phosphorylation | Uniprot | |
| Y34 | Phosphorylation | Uniprot | |
| S35 | Phosphorylation | Uniprot | |
| T36 | Phosphorylation | P51449 (RORC) , O75582 (RPS6KA5) , P31751 (AKT2) , P42345 (MTOR) | Uniprot |
| T37 | Phosphorylation | P49841 (GSK3B) , Q16539 (MAPK14) , P31749 (AKT1) , P42345 (MTOR) , P06493 (CDK1) , P28482 (MAPK1) , Q5S007 (LRRK2) | Uniprot |
| T41 | Phosphorylation | P42345 (MTOR) , P49674 (CSNK1E) | Uniprot |
| S44 | Phosphorylation | P42345 (MTOR) | Uniprot |
| T45 | Phosphorylation | O75582 (RPS6KA5) , P28482 (MAPK1) , P42345 (MTOR) , P31751 (AKT2) , P51449 (RORC) | Uniprot |
| T46 | Phosphorylation | P06493 (CDK1) , Q5S007 (LRRK2) , Q16539 (MAPK14) , P42345 (MTOR) , P31749 (AKT1) , P28482 (MAPK1) , P49841 (GSK3B) | Uniprot |
| T50 | Phosphorylation | P49674 (CSNK1E) | Uniprot |
| Y54 | Phosphorylation | Uniprot | |
| K57 | Ubiquitination | Uniprot | |
| C62 | S-Nitrosylation | Uniprot | |
| R63 | Methylation | Uniprot | |
| S65 | Phosphorylation | P27361 (MAPK3) , P42345 (MTOR) , P28482 (MAPK1) , O75582 (RPS6KA5) , Q8TAI7 (RHEBL1) , P31749 (AKT1) , Q92630 (DYRK2) , Q9P1W9 (PIM2) , P06493 (CDK1) , Q16539 (MAPK14) | Uniprot |
| T68 | Phosphorylation | Uniprot | |
| K69 | Ubiquitination | Uniprot | |
| T70 | Phosphorylation | P28482 (MAPK1) , P06493 (CDK1) , Q16539 (MAPK14) , P42345 (MTOR) , P41279 (MAP3K8) | Uniprot |
| T77 | Phosphorylation | Uniprot | |
| T82 | Phosphorylation | Uniprot | |
| S83 | Phosphorylation | P42345 (MTOR) , P06493 (CDK1) , P28482 (MAPK1) | Uniprot |
| S85 | Phosphorylation | Uniprot | |
| S86 | Phosphorylation | Uniprot | |
| S94 | Phosphorylation | Q13315 (ATM) , Q13535 (ATR) | Uniprot |
| S96 | Phosphorylation | Uniprot | |
| S101 | Phosphorylation | Q92630 (DYRK2) , P42345 (MTOR) | Uniprot |
| S112 | Phosphorylation | P19784 (CSNK2A2) , P68400 (CSNK2A1) , Q13315 (ATM) | Uniprot |
研究背景
Repressor of translation initiation that regulates EIF4E activity by preventing its assembly into the eIF4F complex: hypophosphorylated form competes with EIF4G1/EIF4G3 and strongly binds to EIF4E, leading to repress translation. In contrast, hyperphosphorylated form dissociates from EIF4E, allowing interaction between EIF4G1/EIF4G3 and EIF4E, leading to initiation of translation. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.
Phosphorylated on serine and threonine residues in response to insulin, EGF and PDGF. Phosphorylation at Thr-37, Thr-46, Ser-65 and Thr-70, corresponding to the hyperphosphorylated form, is regulated by mTORC1 and abolishes binding to EIF4E.
Ubiquitinated: when eIF4E levels are low, hypophosphorylated form is ubiquitinated by the BCR(KLHL25) complex, leading to its degradation and serving as a homeostatic mechanism to maintain translation and prevent eIF4E inhibition when eIF4E levels are low. Not ubiquitinated when hyperphosphorylated (at Thr-37, Thr-46, Ser-65 and Thr-70) or associated with eIF4E.
Hypophosphorylated EIF4EBP1 competes with EIF4G1/EIF4G3 to interact with EIF4E; insulin stimulated MAP-kinase (MAPK1 and MAPK3) or mTORC1 phosphorylation of EIF4EBP1 causes dissociation of the complex allowing EIF4G1/EIF4G3 to bind and consequent initiation of translation. Interacts (via TOS motif) with RPTOR; promoting phosphorylation by mTORC1.
The TOS motif mediates interaction with RPTOR, leading to promote phosphorylation by mTORC1 complex.
Belongs to the eIF4E-binding protein family.
研究领域
· Cellular Processes > Cell growth and death > Cellular senescence. (View pathway)
· Environmental Information Processing > Signal transduction > ErbB signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > HIF-1 signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > mTOR signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > AMPK signaling pathway. (View pathway)
· Genetic Information Processing > Translation > RNA transport.
· Human Diseases > Drug resistance: Antineoplastic > EGFR tyrosine kinase inhibitor resistance.
· Human Diseases > Infectious diseases: Viral > Human papillomavirus infection.
· Human Diseases > Cancers: Specific types > Acute myeloid leukemia. (View pathway)
· Human Diseases > Cancers: Overview > Choline metabolism in cancer. (View pathway)
· Organismal Systems > Aging > Longevity regulating pathway. (View pathway)
· Organismal Systems > Endocrine system > Insulin signaling pathway. (View pathway)
限制条款
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