| 产品: | 磷酸化 CDC25B (Ser151) 抗体 |
| 货号: | AF2314 |
| 描述: | Rabbit polyclonal antibody to Phospho-CDC25B (Ser151) |
| 应用: | WB IHC |
| 反应: | Human, Mouse |
| 预测: | Pig, Bovine, Horse, Sheep, Rabbit, Dog |
| 分子量: | 60kDa; 65kD(Calculated). |
| 蛋白号: | P30305 |
| RRID: | AB_2845328 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF2314, RRID:AB_2845328.
展开/折叠
AI604853; Cdc 25B; Cdc25b; CDC25HU2; Cdc25m2; Cell division cycle 25 homolog B; Cell division cycle 25B; Cell division cycle 25B isoform 1; Cell division cycle 25B isoform 2; Cell division cycle 25B isoform 3; Cell division cycle 25B isoform 4; Cell division cycle 25B isoform 5; Dual specificity phosphatase Cdc25B; EC 3.1.3.48; M phase inducer phosphatase 2; M-phase inducer phosphatase 2; MPIP2_HUMAN;
抗原和靶标
- P30305 MPIP2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEVPQPEPAPGSALSPAGVCGGAQRPGHLPGLLLGSHGLLGSPVRAAASSPVTTLTQTMHDLAGLGSETPKSQVGTLLFRSRSRLTHLSLSRRASESSLSSESSESSDAGLCMDSPSPMDPHMAEQTFEQAIQAASRIIRNEQFAIRRFQSMPVRLLGHSPVLRNITNSQAPDGRRKSEAGSGAASSSGEDKENDGFVFKMPWKPTHPSSTHALAEWASRREAFAQRPSSAPDLMCLSPDRKMEVEELSPLALGRFSLTPAEGDTEEDDGFVDILESDLKDDDAVPPGMESLISAPLVKTLEKEEEKDLVMYSKCQRLFRSPSMPCSVIRPILKRLERPQDRDTPVQNKRRRSVTPPEEQQEAEEPKARVLRSKSLCHDEIENLLDSDHRELIGDYSKAFLLQTVDGKHQDLKYISPETMVALLTGKFSNIVDKFVIVDCRYPYEYEGGHIKTAVNLPLERDAESFLLKSPIAPCSLDKRVILIFHCEFSSERGPRMCRFIRERDRAVNDYPSLYYPEMYILKGGYKEFFPQHPNFCEPQDYRPMNHEAFKDELKTFRLKTRSWAGERSRRELCSRLQDQ
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P30305 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S15 | Phosphorylation | Uniprot | |
| S42 | Phosphorylation | Uniprot | |
| S50 | Phosphorylation | P53350 (PLK1) | Uniprot |
| T53 | Phosphorylation | Uniprot | |
| T54 | Phosphorylation | Uniprot | |
| T56 | Phosphorylation | Uniprot | |
| T58 | Phosphorylation | P53350 (PLK1) | Uniprot |
| T69 | Phosphorylation | Uniprot | |
| K71 | Ubiquitination | Uniprot | |
| T86 | Phosphorylation | Uniprot | |
| S89 | Phosphorylation | Uniprot | |
| S91 | Phosphorylation | Uniprot | |
| S97 | Phosphorylation | Uniprot | |
| S101 | Phosphorylation | Uniprot | |
| S115 | Phosphorylation | P45983 (MAPK8) | Uniprot |
| S117 | Phosphorylation | P45983 (MAPK8) | Uniprot |
| T127 | Phosphorylation | P53350 (PLK1) | Uniprot |
| S151 | Phosphorylation | P17612 (PRKACA) | Uniprot |
| S160 | Phosphorylation | P06493 (CDK1) | Uniprot |
| T167 | Phosphorylation | P53350 (PLK1) | Uniprot |
| S169 | Phosphorylation | Q14680 (MELK) , P49137 (MAPKAPK2) | Uniprot |
| K177 | Ubiquitination | Uniprot | |
| S178 | Phosphorylation | Uniprot | |
| S182 | Phosphorylation | Uniprot | |
| S186 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| S187 | Phosphorylation | P68400 (CSNK2A1) | Uniprot |
| S188 | Phosphorylation | Uniprot | |
| K192 | Ubiquitination | Uniprot | |
| K200 | Ubiquitination | Uniprot | |
| K204 | Ubiquitination | Uniprot | |
| S209 | Phosphorylation | P53350 (PLK1) | Uniprot |
| S219 | Phosphorylation | Q14680 (MELK) | Uniprot |
| S229 | Phosphorylation | Uniprot | |
| S230 | Phosphorylation | P17612 (PRKACA) , O14757 (CHEK1) | Uniprot |
| S238 | Phosphorylation | Uniprot | |
| K242 | Sumoylation | Uniprot | |
| K242 | Ubiquitination | Uniprot | |
| S249 | Phosphorylation | Q15759 (MAPK11) | Uniprot |
| T265 | Phosphorylation | P53350 (PLK1) | Uniprot |
| S291 | Phosphorylation | P53350 (PLK1) | Uniprot |
| K299 | Ubiquitination | Uniprot | |
| K303 | Ubiquitination | Uniprot | |
| K307 | Ubiquitination | Uniprot | |
| Y312 | Phosphorylation | Uniprot | |
| S321 | Phosphorylation | P06493 (CDK1) | Uniprot |
| S323 | Phosphorylation | Q16539 (MAPK14) , P17612 (PRKACA) , P49137 (MAPKAPK2) , Q14680 (MELK) | Uniprot |
| S327 | Phosphorylation | Uniprot | |
| K334 | Ubiquitination | Uniprot | |
| T344 | Phosphorylation | Uniprot | |
| K349 | Ubiquitination | Uniprot | |
| S353 | Phosphorylation | P53350 (PLK1) , P49137 (MAPKAPK2) , Q15418 (RPS6KA1) , O14965 (AURKA) , P31749 (AKT1) | Uniprot |
| T355 | Phosphorylation | Q15418 (RPS6KA1) | Uniprot |
| K367 | Sumoylation | Uniprot | |
| K367 | Ubiquitination | Uniprot | |
| S373 | Phosphorylation | Uniprot | |
| K374 | Ubiquitination | Uniprot | |
| S375 | Phosphorylation | P49137 (MAPKAPK2) , Q8TDC3-2 (BRSK1) , Q16539 (MAPK14) , P53350 (PLK1) | Uniprot |
| S387 | Phosphorylation | Uniprot | |
| S397 | Phosphorylation | P53350 (PLK1) | Uniprot |
| K398 | Ubiquitination | Uniprot | |
| T404 | Phosphorylation | P53350 (PLK1) | Uniprot |
| K408 | Ubiquitination | Uniprot | |
| K413 | Ubiquitination | Uniprot | |
| K427 | Ubiquitination | Uniprot | |
| K434 | Ubiquitination | Uniprot | |
| K452 | Ubiquitination | Uniprot | |
| S465 | Phosphorylation | P53350 (PLK1) | Uniprot |
| K469 | Ubiquitination | Uniprot | |
| S470 | Phosphorylation | Uniprot | |
| K479 | Ubiquitination | Uniprot | |
| S513 | Phosphorylation | P53350 (PLK1) | Uniprot |
| K523 | Ubiquitination | Uniprot | |
| K527 | Ubiquitination | Uniprot | |
| K551 | Ubiquitination | Uniprot | |
| K555 | Ubiquitination | Uniprot | |
| T556 | Phosphorylation | Uniprot | |
| S563 | Phosphorylation | O14757 (CHEK1) | Uniprot |
研究背景
Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Directly dephosphorylates CDK1 and stimulates its kinase activity. The three isoforms seem to have a different level of activity.
Phosphorylated by BRSK1 in vitro. Phosphorylated by CHEK1, which inhibits the activity of this protein. Phosphorylation at Ser-353 by AURKA might locally participate in the control of the onset of mitosis. Phosphorylation by MELK at Ser-169 promotes localization to the centrosome and the spindle poles during mitosis. Phosphorylation at Ser-323 and Ser-375 by MAPK14 is required for binding to 14-3-3 proteins.
Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Cytoplasm>Cytoskeleton>Spindle pole.
Interacts with MAPK14 and 14-3-3 proteins.
Belongs to the MPI phosphatase family.
研究领域
· Cellular Processes > Cell growth and death > Cell cycle. (View pathway)
· Environmental Information Processing > Signal transduction > MAPK signaling pathway. (View pathway)
· Human Diseases > Cancers: Overview > MicroRNAs in cancer.
· Organismal Systems > Endocrine system > Progesterone-mediated oocyte maturation.
限制条款
产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。
产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。
Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。产品仅供科学研究使用。不用于诊断和治疗。
产品未经授权不得转售。
Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.