Phospho-AS160 (Ser318) Antibody - #AF2317

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产品: 磷酸化 AS160 (Ser318) 抗体
货号: AF2317
描述: Rabbit polyclonal antibody to Phospho-AS160 (Ser318)
应用: WB IHC
反应: Human, Mouse, Rat
预测: Bovine, Horse, Sheep, Rabbit, Dog
分子量: 160kDa; 147kD(Calculated).
蛋白号: O60343
RRID: AB_2845331

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
克隆:
Polyclonal
特异性:
Phospho-AS160 (Ser318) Antibody detects endogenous levels of AS160 only when phosphorylated at Ser318.
RRID:
AB_2845331
引用格式: Affinity Biosciences Cat# AF2317, RRID:AB_2845331.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Acrg embryonic lethality (mouse) minimal region ortholog; Acrg embryonic lethality minimal region ortholog; Acrg embryonic lethality mouse minimal region ortholog; Akt substrate of 160 kDa; AS 160; AS160; BUB2; CDC16; KIAA0603; NIDDM5; TBC (Tre 2 BUB2 CDC16) domain containing protein; TBC Tre 2 BUB2 CDC16 domain containing protein; TBC1 D4; TBC1 domain family member 4; Tbc1d4; TBCD4_HUMAN; Tre-2;

抗原和靶标

免疫原:
Uniprot:

O60343(TBCD4_HUMAN) >>Visit HPA database.

基因/基因ID:
TBC1D4(9882)
表达:
O60343 TBCD4_HUMAN:

Widely expressed. Isoform 2 is the highest overexpressed in most tissues. Isoform 1 is highly expressed in skeletal muscle and heart, but was not detectable in the liver nor in adipose tissue. Isoform 2 is strongly expressed in adrenal and thyroid gland, and also in lung, kidney, colon, brain and adipose tissue. Isoform 2 is moderately expressed in skeletal muscle. Expressed in pancreatic Langerhans islets, including beta cells (at protein level). Expression is decreased by twofold in pancreatic islets in type 2 diabetes patients compared to control subjects. Up-regulated in T-cells from patients with atopic dermatitis.

序列:
MEPPSCIQDEPFPHPLEPEPGVSAQPGPGKPSDKRFRLWYVGGSCLDHRTTLPMLPWLMAEIRRRSQKPEAGGCGAPAAREVILVLSAPFLRCVPAPGAGASGGTSPSATQPNPAVFIFEHKAQHISRFIHNSHDLTYFAYLIKAQPDDPESQMACHVFRATDPSQVPDVISSIRQLSKAAMKEDAKPSKDNEDAFYNSQKFEVLYCGKVTVTHKKAPSSLIDDCMEKFSLHEQQRLKIQGEQRGPDPGEDLADLEVVVPGSPGDCLPEEADGTDTHLGLPAGASQPALTSSRVCFPERILEDSGFDEQQEFRSRCSSVTGVQRRVHEGSQKSQPRRRHASAPSHVQPSDSEKNRTMLFQVGRFEINLISPDTKSVVLEKNFKDISSCSQGIKHVDHFGFICRESPEPGLSQYICYVFQCASESLVDEVMLTLKQAFSTAAALQSAKTQIKLCEACPMHSLHKLCERIEGLYPPRAKLVIQRHLSSLTDNEQADIFERVQKMKPVSDQEENELVILHLRQLCEAKQKTHVHIGEGPSTISNSTIPENATSSGRFKLDILKNKAKRSLTSSLENIFSRGANRMRGRLGSVDSFERSNSLASEKDYSPGDSPPGTPPASPPSSAWQTFPEEDSDSPQFRRRAHTFSHPPSSTKRKLNLQDGRAQGVRSPLLRQSSSEQCSNLSSVRRMYKESNSSSSLPSLHTSFSAPSFTAPSFLKSFYQNSGRLSPQYENEIRQDTASESSDGEGRKRTSSTCSNESLSVGGTSVTPRRISWRQRIFLRVASPMNKSPSAMQQQDGLDRNELLPLSPLSPTMEEEPLVVFLSGEDDPEKIEERKKSKELRSLWRKAIHQQILLLRMEKENQKLEASRDELQSRKVKLDYEEVGACQKEVLITWDKKLLNCRAKIRCDMEDIHTLLKEGVPKSRRGEIWQFLALQYRLRHRLPNKQQPPDISYKELLKQLTAQQHAILVDLGRTFPTHPYFSVQLGPGQLSLFNLLKAYSLLDKEVGYCQGISFVAGVLLLHMSEEQAFEMLKFLMYDLGFRKQYRPDMMSLQIQMYQLSRLLHDYHRDLYNHLEENEISPSLYAAPWFLTLFASQFSLGFVARVFDIIFLQGTEVIFKVALSLLSSQETLIMECESFENIVEFLKNTLPDMNTSEMEKIITQVFEMDISKQLHAYEVEYHVLQDELQESSYSCEDSETLEKLERANSQLKRQNMDLLEKLQVAHTKIQALESNLENLLTRETKMKSLIRTLEQEKMAYQKTVEQLRKLLPADALVNCDLLLRDLNCNPNNKAKIGNKP

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Chicken
75
Xenopus
67
Pig
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - O60343 作为底物

Site PTM Type Enzyme
M1 Acetylation
S44 Phosphorylation
S66 Phosphorylation
K68 Ubiquitination
S102 Phosphorylation
K228 Ubiquitination
K238 Ubiquitination
S262 Phosphorylation
T274 Phosphorylation
S285 Phosphorylation
S304 Phosphorylation
S314 Phosphorylation
S317 Phosphorylation
S318 Phosphorylation O00141 (SGK1) , P31749 (AKT1)
T320 Phosphorylation
S330 Phosphorylation
S341 Phosphorylation Q15418 (RPS6KA1)
S344 Phosphorylation
S349 Phosphorylation
S351 Phosphorylation
T356 Phosphorylation
S370 Phosphorylation
K383 Ubiquitination
K477 Acetylation
S485 Phosphorylation O14757 (CHEK1)
S486 Phosphorylation
T488 Phosphorylation
S551 Phosphorylation
K555 Ubiquitination
K560 Ubiquitination
S566 Phosphorylation
T568 Phosphorylation O00141 (SGK1)
S569 Phosphorylation
S570 Phosphorylation
S576 Phosphorylation
S588 Phosphorylation O14757 (CHEK1) , P31749 (AKT1) , O00141 (SGK1)
S591 Phosphorylation
S595 Phosphorylation
S597 Phosphorylation
S600 Phosphorylation
S609 Phosphorylation
T613 Phosphorylation
S617 Phosphorylation
S621 Phosphorylation
S633 Phosphorylation
T642 Phosphorylation O00141 (SGK1) , P31749 (AKT1)
S644 Phosphorylation
S648 Phosphorylation
S649 Phosphorylation
S666 Phosphorylation
S672 Phosphorylation
S673 Phosphorylation
S678 Phosphorylation
T701 Phosphorylation
S704 Phosphorylation
S707 Phosphorylation
T749 Phosphorylation
S750 Phosphorylation
S751 Phosphorylation O00141 (SGK1) , Q15418 (RPS6KA1)
T752 Phosphorylation
S754 Phosphorylation
S757 Phosphorylation
S759 Phosphorylation
T763 Phosphorylation
S764 Phosphorylation
T766 Phosphorylation
S782 Phosphorylation
S787 Phosphorylation
S789 Phosphorylation
S806 Phosphorylation
S809 Phosphorylation
Y1191 Phosphorylation
S1207 Phosphorylation
S1246 Phosphorylation
K1255 Ubiquitination
K1267 Ubiquitination

研究背景

功能:

May act as a GTPase-activating protein for RAB2A, RAB8A, RAB10 and RAB14. Isoform 2 promotes insulin-induced glucose transporter SLC2A4/GLUT4 translocation at the plasma membrane, thus increasing glucose uptake.

翻译修饰:

Phosphorylated by AKT1; insulin-induced. Also phosphorylated by AMPK in response to insulin. Insulin-stimulated phosphorylation is required for SLC2A4/GLUT4 translocation. Has no effect on SLC2A4/GLUT4 internalization. Physiological hyperinsulinemia increases phosphorylation in skeletal muscle. Insulin-stimulated phosphorylation is reduced by 39% in type 2 diabetic patients.

细胞定位:

Cytoplasm.
Note: Isoform 2 shows a cytoplasmic perinuclear localization in a myoblastic cell line in resting and insulin-stimulated cells.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Widely expressed. Isoform 2 is the highest overexpressed in most tissues. Isoform 1 is highly expressed in skeletal muscle and heart, but was not detectable in the liver nor in adipose tissue. Isoform 2 is strongly expressed in adrenal and thyroid gland, and also in lung, kidney, colon, brain and adipose tissue. Isoform 2 is moderately expressed in skeletal muscle. Expressed in pancreatic Langerhans islets, including beta cells (at protein level). Expression is decreased by twofold in pancreatic islets in type 2 diabetes patients compared to control subjects. Up-regulated in T-cells from patients with atopic dermatitis.

研究领域

· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.

· Organismal Systems > Endocrine system > Thyroid hormone signaling pathway.   (View pathway)

文献引用

1). Akt Phosphorylation Influences Persistent Chlamydial Infection and Chlamydia-Induced Golgi Fragmentation Without Involving Rab14. Frontiers in Cellular and Infection Microbiology (PubMed: 34169005) [IF=5.7]
2). Central GLP-1 contributes to improved cognitive function and brain glucose uptake after duodenum-jejunum bypass on obese and diabetic rats. American Journal of Physiology-Endocrinology and Metabolism (PubMed: 34370593) [IF=5.1]

Application: WB    Species: Rat    Sample: brain

Figure 9. The effect of glucagon-like peptide-1 (GLP-1) receptor (Glp-1r) knockdown (KD) on brain glucose uptake and validation of Glp-1r KD. A and B: representative rat brain positron emission tomography (PET) images and comparisons on the glucose uptake of the whole brain on each hemicerebrum among subgroups. C: immunoblotting of brain GLP-1R in different regions. D: immunoblotting of glucose uptake-related proteins. Multiple comparisons in two-way ANOVA were used, and data are expressed as means ± SD. ****P < 0.0001 (n = 4 for animals in each group). AS160, Akt substrate of 160 kDa; DJB, duodenum-jejunum bypass; GLUT, glucose transporter; SUV, standardized uptake value.
3). Interleukin-4 Restores Insulin Sensitivity in Insulin-Resistant Osteoblasts by Increasing the Expression of Insulin Receptor Substrate 1. Biochemistry (Moscow) (PubMed: 32564738) [IF=2.8]

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