产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF2318, RRID:AB_2845332.
展开/折叠
Acrg embryonic lethality (mouse) minimal region ortholog; Acrg embryonic lethality minimal region ortholog; Acrg embryonic lethality mouse minimal region ortholog; Akt substrate of 160 kDa; AS 160; AS160; BUB2; CDC16; KIAA0603; NIDDM5; TBC (Tre 2 BUB2 CDC16) domain containing protein; TBC Tre 2 BUB2 CDC16 domain containing protein; TBC1 D4; TBC1 domain family member 4; Tbc1d4; TBCD4_HUMAN; Tre-2;
抗原和靶标
Widely expressed. Isoform 2 is the highest overexpressed in most tissues. Isoform 1 is highly expressed in skeletal muscle and heart, but was not detectable in the liver nor in adipose tissue. Isoform 2 is strongly expressed in adrenal and thyroid gland, and also in lung, kidney, colon, brain and adipose tissue. Isoform 2 is moderately expressed in skeletal muscle. Expressed in pancreatic Langerhans islets, including beta cells (at protein level). Expression is decreased by twofold in pancreatic islets in type 2 diabetes patients compared to control subjects. Up-regulated in T-cells from patients with atopic dermatitis.
- O60343 TBCD4_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEPPSCIQDEPFPHPLEPEPGVSAQPGPGKPSDKRFRLWYVGGSCLDHRTTLPMLPWLMAEIRRRSQKPEAGGCGAPAAREVILVLSAPFLRCVPAPGAGASGGTSPSATQPNPAVFIFEHKAQHISRFIHNSHDLTYFAYLIKAQPDDPESQMACHVFRATDPSQVPDVISSIRQLSKAAMKEDAKPSKDNEDAFYNSQKFEVLYCGKVTVTHKKAPSSLIDDCMEKFSLHEQQRLKIQGEQRGPDPGEDLADLEVVVPGSPGDCLPEEADGTDTHLGLPAGASQPALTSSRVCFPERILEDSGFDEQQEFRSRCSSVTGVQRRVHEGSQKSQPRRRHASAPSHVQPSDSEKNRTMLFQVGRFEINLISPDTKSVVLEKNFKDISSCSQGIKHVDHFGFICRESPEPGLSQYICYVFQCASESLVDEVMLTLKQAFSTAAALQSAKTQIKLCEACPMHSLHKLCERIEGLYPPRAKLVIQRHLSSLTDNEQADIFERVQKMKPVSDQEENELVILHLRQLCEAKQKTHVHIGEGPSTISNSTIPENATSSGRFKLDILKNKAKRSLTSSLENIFSRGANRMRGRLGSVDSFERSNSLASEKDYSPGDSPPGTPPASPPSSAWQTFPEEDSDSPQFRRRAHTFSHPPSSTKRKLNLQDGRAQGVRSPLLRQSSSEQCSNLSSVRRMYKESNSSSSLPSLHTSFSAPSFTAPSFLKSFYQNSGRLSPQYENEIRQDTASESSDGEGRKRTSSTCSNESLSVGGTSVTPRRISWRQRIFLRVASPMNKSPSAMQQQDGLDRNELLPLSPLSPTMEEEPLVVFLSGEDDPEKIEERKKSKELRSLWRKAIHQQILLLRMEKENQKLEASRDELQSRKVKLDYEEVGACQKEVLITWDKKLLNCRAKIRCDMEDIHTLLKEGVPKSRRGEIWQFLALQYRLRHRLPNKQQPPDISYKELLKQLTAQQHAILVDLGRTFPTHPYFSVQLGPGQLSLFNLLKAYSLLDKEVGYCQGISFVAGVLLLHMSEEQAFEMLKFLMYDLGFRKQYRPDMMSLQIQMYQLSRLLHDYHRDLYNHLEENEISPSLYAAPWFLTLFASQFSLGFVARVFDIIFLQGTEVIFKVALSLLSSQETLIMECESFENIVEFLKNTLPDMNTSEMEKIITQVFEMDISKQLHAYEVEYHVLQDELQESSYSCEDSETLEKLERANSQLKRQNMDLLEKLQVAHTKIQALESNLENLLTRETKMKSLIRTLEQEKMAYQKTVEQLRKLLPADALVNCDLLLRDLNCNPNNKAKIGNKP
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - O60343 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| S44 | Phosphorylation | Uniprot | |
| S66 | Phosphorylation | Uniprot | |
| K68 | Ubiquitination | Uniprot | |
| S102 | Phosphorylation | Uniprot | |
| K228 | Ubiquitination | Uniprot | |
| K238 | Ubiquitination | Uniprot | |
| S262 | Phosphorylation | Uniprot | |
| T274 | Phosphorylation | Uniprot | |
| S285 | Phosphorylation | Uniprot | |
| S304 | Phosphorylation | Uniprot | |
| S314 | Phosphorylation | Uniprot | |
| S317 | Phosphorylation | Uniprot | |
| S318 | Phosphorylation | O00141 (SGK1) , P31749 (AKT1) | Uniprot |
| T320 | Phosphorylation | Uniprot | |
| S330 | Phosphorylation | Uniprot | |
| S341 | Phosphorylation | Q15418 (RPS6KA1) | Uniprot |
| S344 | Phosphorylation | Uniprot | |
| S349 | Phosphorylation | Uniprot | |
| S351 | Phosphorylation | Uniprot | |
| T356 | Phosphorylation | Uniprot | |
| S370 | Phosphorylation | Uniprot | |
| K383 | Ubiquitination | Uniprot | |
| K477 | Acetylation | Uniprot | |
| S485 | Phosphorylation | O14757 (CHEK1) | Uniprot |
| S486 | Phosphorylation | Uniprot | |
| T488 | Phosphorylation | Uniprot | |
| S551 | Phosphorylation | Uniprot | |
| K555 | Ubiquitination | Uniprot | |
| K560 | Ubiquitination | Uniprot | |
| S566 | Phosphorylation | Uniprot | |
| T568 | Phosphorylation | O00141 (SGK1) | Uniprot |
| S569 | Phosphorylation | Uniprot | |
| S570 | Phosphorylation | Uniprot | |
| S576 | Phosphorylation | Uniprot | |
| S588 | Phosphorylation | O14757 (CHEK1) , P31749 (AKT1) , O00141 (SGK1) | Uniprot |
| S591 | Phosphorylation | Uniprot | |
| S595 | Phosphorylation | Uniprot | |
| S597 | Phosphorylation | Uniprot | |
| S600 | Phosphorylation | Uniprot | |
| S609 | Phosphorylation | Uniprot | |
| T613 | Phosphorylation | Uniprot | |
| S617 | Phosphorylation | Uniprot | |
| S621 | Phosphorylation | Uniprot | |
| S633 | Phosphorylation | Uniprot | |
| T642 | Phosphorylation | O00141 (SGK1) , P31749 (AKT1) | Uniprot |
| S644 | Phosphorylation | Uniprot | |
| S648 | Phosphorylation | Uniprot | |
| S649 | Phosphorylation | Uniprot | |
| S666 | Phosphorylation | Uniprot | |
| S672 | Phosphorylation | Uniprot | |
| S673 | Phosphorylation | Uniprot | |
| S678 | Phosphorylation | Uniprot | |
| T701 | Phosphorylation | Uniprot | |
| S704 | Phosphorylation | Uniprot | |
| S707 | Phosphorylation | Uniprot | |
| T749 | Phosphorylation | Uniprot | |
| S750 | Phosphorylation | Uniprot | |
| S751 | Phosphorylation | O00141 (SGK1) , Q15418 (RPS6KA1) | Uniprot |
| T752 | Phosphorylation | Uniprot | |
| S754 | Phosphorylation | Uniprot | |
| S757 | Phosphorylation | Uniprot | |
| S759 | Phosphorylation | Uniprot | |
| T763 | Phosphorylation | Uniprot | |
| S764 | Phosphorylation | Uniprot | |
| T766 | Phosphorylation | Uniprot | |
| S782 | Phosphorylation | Uniprot | |
| S787 | Phosphorylation | Uniprot | |
| S789 | Phosphorylation | Uniprot | |
| S806 | Phosphorylation | Uniprot | |
| S809 | Phosphorylation | Uniprot | |
| Y1191 | Phosphorylation | Uniprot | |
| S1207 | Phosphorylation | Uniprot | |
| S1246 | Phosphorylation | Uniprot | |
| K1255 | Ubiquitination | Uniprot | |
| K1267 | Ubiquitination | Uniprot |
研究背景
May act as a GTPase-activating protein for RAB2A, RAB8A, RAB10 and RAB14. Isoform 2 promotes insulin-induced glucose transporter SLC2A4/GLUT4 translocation at the plasma membrane, thus increasing glucose uptake.
Phosphorylated by AKT1; insulin-induced. Also phosphorylated by AMPK in response to insulin. Insulin-stimulated phosphorylation is required for SLC2A4/GLUT4 translocation. Has no effect on SLC2A4/GLUT4 internalization. Physiological hyperinsulinemia increases phosphorylation in skeletal muscle. Insulin-stimulated phosphorylation is reduced by 39% in type 2 diabetic patients.
Cytoplasm.
Note: Isoform 2 shows a cytoplasmic perinuclear localization in a myoblastic cell line in resting and insulin-stimulated cells.
Widely expressed. Isoform 2 is the highest overexpressed in most tissues. Isoform 1 is highly expressed in skeletal muscle and heart, but was not detectable in the liver nor in adipose tissue. Isoform 2 is strongly expressed in adrenal and thyroid gland, and also in lung, kidney, colon, brain and adipose tissue. Isoform 2 is moderately expressed in skeletal muscle. Expressed in pancreatic Langerhans islets, including beta cells (at protein level). Expression is decreased by twofold in pancreatic islets in type 2 diabetes patients compared to control subjects. Up-regulated in T-cells from patients with atopic dermatitis.
研究领域
· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.
· Organismal Systems > Endocrine system > Thyroid hormone signaling pathway. (View pathway)
限制条款
产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。
产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。
Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。产品仅供科学研究使用。不用于诊断和治疗。
产品未经授权不得转售。
Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.