Phospho-Dab1 (Tyr220) Antibody - #AF2335

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产品: 磷酸化 Dab1 (Tyr220) 抗体
货号: AF2335
描述: Rabbit polyclonal antibody to Phospho-Dab1 (Tyr220)
应用: WB IHC
反应: Human, Mouse, Rat
预测: Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
分子量: 110 kDa; 64kD(Calculated).
蛋白号: O75553
RRID: AB_2845349

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 100ul RMB¥ 2800 现货
 200ul RMB¥ 3800 现货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Bovine(100%), Horse(92%), Sheep(92%), Rabbit(100%), Dog(100%), Xenopus(82%)
克隆:
Polyclonal
特异性:
Phospho-Dab1 (Tyr220) Antibody detects endogenous levels of Dab1 only when phosphorylated at Tyr220.
RRID:
AB_2845349
引用格式: Affinity Biosciences Cat# AF2335, RRID:AB_2845349.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Dab 1; Dab reelin signal transducer 1; Dab, reelin signal transducer, homolog 1 (Drosophila); Dab1; DAB1_HUMAN; Disabled homolog 1; Disabled homolog 1 Drosophila; Scm; Scr; Scrambler; Yot; Yotari;

抗原和靶标

免疫原:
Uniprot:

O75553(DAB1_HUMAN) >>Visit HPA database.

基因/基因ID:
DAB1(1600)
表达:
O75553 DAB1_HUMAN:

Mainly expressed in brain.

序列:
MSTETELQVAVKTSAKKDSRKKGQDRSEATLIKRFKGEGVRYKAKLIGIDEVSAARGDKLCQDSMMKLKGVVAGARSKGEHKQKIFLTISFGGIKIFDEKTGALQHHHAVHEISYIAKDITDHRAFGYVCGKEGNHRFVAIKTAQAAEPVILDLRDLFQLIYELKQREELEKKAQKDKQCEQAVYQTILEEDVEDPVYQYIVFEAGHEPIRDPETEENIYQVPTSQKKEGVYDVPKSQPVSNGYSFEDFEERFAAATPNRNLPTDFDEIFEATKAVTQLELFGDMSTPPDITSPPTPATPGDAFIPSSSQTLPASADVFSSVPFGTAAVPSGYVAMGAVLPSFWGQQPLVQQQMVMGAQPPVAQVMPGAQPIAWGQPGLFPATQQPWPTVAGQFPPAAFMPTQTVMPLPAAMFQGPLTPLATVPGTSDSTRSSPQTDKPRQKMGKETFKDFQMAQPPPVPSRKPDQPSLTCTSEAFSSYFNKVGVAQDTDDCDDFDISQLNLTPVTSTTPSTNSPPTPAPRQSSPSKSSASHASDPTTDDIFEEGFESPSKSEEQEAPDGSQASSNSDPFGEPSGEPSGDNISPQAGS

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Bovine
100
Dog
100
Rabbit
100
Horse
92
Sheep
92
Xenopus
82
Pig
73
Chicken
73
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - O75553 作为底物

Site PTM Type Enzyme
K43 Ubiquitination
K45 Ubiquitination
K84 Sumoylation
Y198 Phosphorylation
Y220 Phosphorylation
Y232 Phosphorylation
S433 Phosphorylation
T506 Phosphorylation
S524 Phosphorylation P24941 (CDK2)
S526 Phosphorylation
S548 Phosphorylation

研究背景

功能:

Adapter molecule functioning in neural development. May regulate SIAH1 activity.

翻译修饰:

Phosphorylated on Tyr-198 and Tyr-220 upon reelin induction in embryonic neurons. Also phosphorylated on Ser-524 independently of reelin signaling.

组织特异性:

Mainly expressed in brain.

亚基结构:

Associates with the SH2 domains of SRC, FYN and ABL (By similarity). Interacts (phosphorylated on tyrosine residues) with CRK and CRKL (via respective SH2 domain) (By similarity). Interacts with SIAH1, LRP8 and VLDLR (By similarity). Interacts with LRP1. Interacts with APLP1 (via NPXY motif) (By similarity). Interacts with DAB2IP (By similarity).

蛋白家族:

The PID domain specifically binds to the Asn-Pro-Xaa-Tyr(P) motif found in many tyrosine-phosphorylated proteins.

文献引用

1). Rnf-213 Knockout Induces Pericyte Reduction and Blood-Brain Barrier Impairment in Mouse. Molecular Neurobiology (PubMed: 37438553) [IF=5.1]

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