产品: 磷酸化 Doublecortin (Ser297) 抗体
货号: AF2338
描述: Rabbit polyclonal antibody to Phospho-Doublecortin (Ser297)
应用: WB IHC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
分子量: 45kDa; 41kD(Calculated).
蛋白号: O43602
RRID: AB_2845352

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   规格 价格 库存
 100ul RMB¥ 2800 现货
 200ul RMB¥ 3800 现货

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产品描述

来源:
Rabbit
应用:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(92%), Bovine(83%), Horse(92%), Sheep(83%), Rabbit(92%), Dog(92%), Chicken(83%)
克隆:
Polyclonal
特异性:
Phospho-Doublecortin (Ser297) Antibody detects endogenous levels of Doublecortin only when phosphorylated at Ser297.
RRID:
AB_2845352
引用格式: Affinity Biosciences Cat# AF2338, RRID:AB_2845352.
偶联:
Unconjugated.
纯化:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

DBCN; Dbct; DC; DCX; DCX_HUMAN; Doublecortex; Doublin; FLJ51296; Lis X; Lis-X; Lissencephalin X; Lissencephalin-X; Lissencephaly X linked; Lissencephaly X linked doublecortin; LISX; Neuronal migration protein doublecortin; OTTHUMP00000023859; OTTHUMP00000023860; OTTHUMP00000216315; OTTHUMP00000216316; SCLH; XLIS;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
表达:
O43602 DCX_HUMAN:

Highly expressed in neuronal cells of fetal brain (in the majority of cells of the cortical plate, intermediate zone and ventricular zone), but not expressed in other fetal tissues. In the adult, highly expressed in the brain frontal lobe, but very low expression in other regions of brain, and not detected in heart, placenta, lung, liver, skeletal muscles, kidney and pancreas.

序列:
MELDFGHFDERDKTSRNMRGSRMNGLPSPTHSAHCSFYRTRTLQALSNEKKAKKVRFYRNGDRYFKGIVYAVSSDRFRSFDALLADLTRSLSDNINLPQGVRYIYTIDGSRKIGSMDELEEGESYVCSSDNFFKKVEYTKNVNPNWSVNVKTSANMKAPQSLASSNSAQARENKDFVRPKLVTIIRSGVKPRKAVRVLLNKKTAHSFEQVLTDITEAIKLETGVVKKLYTLDGKQVTCLHDFFGDDDVFIACGPEKFRYAQDDFSLDENECRVMKGNPSATAGPKASPTPQKTSAKSPGPMRRSKSPADSGNDQDANGTSSSQLSTPKSKQSPISTPTSPGSLRKHKDLYLPLSLDDSDSLGDSM

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Pig
92
Horse
92
Dog
92
Rabbit
92
Bovine
83
Sheep
83
Chicken
83
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - O43602 作为底物

Site PTM Type Enzyme
S28 Phosphorylation
T30 Phosphorylation
S47 Phosphorylation
K50 Acetylation
K51 Acetylation
Y64 Phosphorylation
S74 Phosphorylation
Y103 Phosphorylation
Y105 Phosphorylation
S128 Phosphorylation P17612 (PRKACA)
T203 Phosphorylation
S206 Phosphorylation
T222 Phosphorylation
S287 Phosphorylation
S297 Phosphorylation
S306 Phosphorylation
S320 Phosphorylation
S325 Phosphorylation
S329 Phosphorylation
S332 Phosphorylation P49841 (GSK3B)
S335 Phosphorylation
S339 Phosphorylation
K345 Acetylation
K347 Acetylation

研究背景

功能:

Microtubule-associated protein required for initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. May act by competing with the putative neuronal protein kinase DCLK1 in binding to a target protein. May in that way participate in a signaling pathway that is crucial for neuronal interaction before and during migration, possibly as part of a calcium ion-dependent signal transduction pathway. May be part with PAFAH1B1/LIS-1 of overlapping, but distinct, signaling pathways that promote neuronal migration.

翻译修饰:

Phosphorylation by MARK1, MARK2 and PKA regulates its ability to bind microtubules (By similarity). Phosphorylation at Ser-265 and Ser-297 seems to occur only in neonatal brain, the levels falling precipitously by postnatal day 21 (By similarity).

Ubiquitinated by MDM2, leading to its degradation by the proteasome. Ubiquitinated by MDM2 and subsequent degradation leads to reduce the dendritic spine density of olfactory bulb granule cells.

细胞定位:

Cytoplasm. Cell projection>Neuron projection.
Note: Localizes at neurite tips.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Highly expressed in neuronal cells of fetal brain (in the majority of cells of the cortical plate, intermediate zone and ventricular zone), but not expressed in other fetal tissues. In the adult, highly expressed in the brain frontal lobe, but very low expression in other regions of brain, and not detected in heart, placenta, lung, liver, skeletal muscles, kidney and pancreas.

亚基结构:

Interacts with tubulin. Interacts with USP9X.

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