| 产品: | 磷酸化 eIF4G1 (Ser1147) 抗体 |
| 货号: | AF2341 |
| 描述: | Rabbit polyclonal antibody to Phospho-eIF4G1 (Ser1147) |
| 应用: | WB IHC |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Bovine, Horse, Sheep, Rabbit, Dog |
| 分子量: | 220kDa; 175kD(Calculated). |
| 蛋白号: | Q04637 |
| RRID: | AB_2845355 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF2341, RRID:AB_2845355.
展开/折叠
DKFZp686A1451; eIF 4 gamma 1; eIF 4G 1; eIF 4G1; eIF-4-gamma 1; eIF-4G 1; eIF-4G1; EIF4 gamma; EIF4F; EIF4G; EIF4G1; EIF4GI; Eukaryotic translation initiation factor 4 gamma 1; IF4G1_HUMAN; p220;
抗原和靶标
- Q04637 IF4G1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MNKAPQSTGPPPAPSPGLPQPAFPPGQTAPVVFSTPQATQMNTPSQPRQHFYPSRAQPPSSAASRVQSAAPARPGPAAHVYPAGSQVMMIPSQISYPASQGAYYIPGQGRSTYVVPTQQYPVQPGAPGFYPGASPTEFGTYAGAYYPAQGVQQFPTGVAPTPVLMNQPPQIAPKRERKTIRIRDPNQGGKDITEEIMSGARTASTPTPPQTGGGLEPQANGETPQVAVIVRPDDRSQGAIIADRPGLPGPEHSPSESQPSSPSPTPSPSPVLEPGSEPNLAVLSIPGDTMTTIQMSVEESTPISRETGEPYRLSPEPTPLAEPILEVEVTLSKPVPESEFSSSPLQAPTPLASHTVEIHEPNGMVPSEDLEPEVESSPELAPPPACPSESPVPIAPTAQPEELLNGAPSPPAVDLSPVSEPEEQAKEVTASMAPPTIPSATPATAPSATSPAQEEEMEEEEEEEEGEAGEAGEAESEKGGEELLPPESTPIPANLSQNLEAAAATQVAVSVPKRRRKIKELNKKEAVGDLLDAFKEANPAVPEVENQPPAGSNPGPESEGSGVPPRPEEADETWDSKEDKIHNAENIQPGEQKYEYKSDQWKPLNLEEKKRYDREFLLGFQFIFASMQKPEGLPHISDVVLDKANKTPLRPLDPTRLQGINCGPDFTPSFANLGRTTLSTRGPPRGGPGGELPRGPAGLGPRRSQQGPRKEPRKIIATVLMTEDIKLNKAEKAWKPSSKRTAADKDRGEEDADGSKTQDLFRRVRSILNKLTPQMFQQLMKQVTQLAIDTEERLKGVIDLIFEKAISEPNFSVAYANMCRCLMALKVPTTEKPTVTVNFRKLLLNRCQKEFEKDKDDDEVFEKKQKEMDEAATAEERGRLKEELEEARDIARRRSLGNIKFIGELFKLKMLTEAIMHDCVVKLLKNHDEESLECLCRLLTTIGKDLDFEKAKPRMDQYFNQMEKIIKEKKTSSRIRFMLQDVLDLRGSNWVPRRGDQGPKTIDQIHKEAEMEEHREHIKVQQLMAKGSDKRRGGPPGPPISRGLPLVDDGGWNTVPISKGSRPIDTSRLTKITKPGSIDSNNQLFAPGGRLSWGKGSSGGSGAKPSDAASEAARPATSTLNRFSALQQAVPTESTDNRRVVQRSSLSRERGEKAGDRGDRLERSERGGDRGDRLDRARTPATKRSFSKEVEERSRERPSQPEGLRKAASLTEDRDRGRDAVKREAALPPVSPLKAALSEEELEKKSKAIIEEYLHLNDMKEAVQCVQELASPSLLFIFVRHGVESTLERSAIAREHMGQLLHQLLCAGHLSTAQYYQGLYEILELAEDMEIDIPHVWLYLAELVTPILQEGGVPMGELFREITKPLRPLGKAASLLLEILGLLCKSMGPKKVGTLWREAGLSWKEFLPEGQDIGAFVAEQKVEYTLGEESEAPGQRALPSEELNRQLEKLLKEGSSNQRVFDWIEANLSEQQIVSNTLVRALMTAVCYSAIIFETPLRVDVAVLKARAKLLQKYLCDEQKELQALYALQALVVTLEQPPNLLRMFFDALYDEDVVKEDAFYSWESSKDPAEQQGKGVALKSVTAFFKWLREAEEESDHN
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q04637 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S15 | Phosphorylation | Uniprot | |
| T43 | Phosphorylation | Uniprot | |
| R48 | Methylation | Uniprot | |
| Y52 | Phosphorylation | Uniprot | |
| R55 | Methylation | Uniprot | |
| S61 | O-Glycosylation | Uniprot | |
| R65 | Methylation | Uniprot | |
| R73 | Methylation | Uniprot | |
| Y103 | Phosphorylation | Uniprot | |
| R110 | Methylation | Uniprot | |
| T179 | Phosphorylation | Uniprot | |
| K190 | Acetylation | Uniprot | |
| T202 | Phosphorylation | Uniprot | |
| S204 | Phosphorylation | Uniprot | |
| T205 | Phosphorylation | Uniprot | |
| T207 | Phosphorylation | Uniprot | |
| T211 | Phosphorylation | Uniprot | |
| T223 | Phosphorylation | Uniprot | |
| S276 | Phosphorylation | Uniprot | |
| T301 | Phosphorylation | Uniprot | |
| S304 | Phosphorylation | Uniprot | |
| Y311 | Phosphorylation | Uniprot | |
| S314 | Phosphorylation | Uniprot | |
| T318 | Phosphorylation | Uniprot | |
| S343 | Phosphorylation | Uniprot | |
| T349 | Phosphorylation | Uniprot | |
| S353 | Phosphorylation | Uniprot | |
| S450 | Phosphorylation | Uniprot | |
| S496 | Phosphorylation | Uniprot | |
| K519 | Sumoylation | Uniprot | |
| K524 | Ubiquitination | Uniprot | |
| K580 | Ubiquitination | Uniprot | |
| K593 | Ubiquitination | Uniprot | |
| Y594 | Phosphorylation | Uniprot | |
| K597 | Ubiquitination | Uniprot | |
| S598 | Phosphorylation | Uniprot | |
| K602 | Ubiquitination | Uniprot | |
| K609 | Ubiquitination | Uniprot | |
| K646 | Ubiquitination | Uniprot | |
| T647 | Phosphorylation | P24941 (CDK2) | Uniprot |
| T667 | Phosphorylation | Uniprot | |
| S669 | Phosphorylation | Uniprot | |
| R675 | Methylation | Uniprot | |
| T676 | Phosphorylation | Uniprot | |
| R681 | Methylation | Uniprot | |
| R685 | Methylation | Uniprot | |
| R694 | Methylation | Uniprot | |
| R702 | Methylation | Uniprot | |
| S704 | Phosphorylation | Uniprot | |
| K732 | Ubiquitination | Uniprot | |
| K735 | Ubiquitination | Uniprot | |
| K756 | Ubiquitination | Uniprot | |
| K770 | Ubiquitination | Uniprot | |
| K781 | Ubiquitination | Uniprot | |
| K795 | Ubiquitination | Uniprot | |
| K832 | Ubiquitination | Uniprot | |
| K863 | Ubiquitination | Uniprot | |
| R877 | Methylation | Uniprot | |
| K881 | Ubiquitination | Uniprot | |
| S895 | Phosphorylation | Q13177 (PAK2) | Uniprot |
| K900 | Ubiquitination | Uniprot | |
| K907 | Ubiquitination | Uniprot | |
| K925 | Ubiquitination | Uniprot | |
| K944 | Ubiquitination | Uniprot | |
| K950 | Acetylation | Uniprot | |
| K950 | Ubiquitination | Uniprot | |
| R954 | Methylation | Uniprot | |
| K964 | Acetylation | Uniprot | |
| K964 | Ubiquitination | Uniprot | |
| K967 | Ubiquitination | Uniprot | |
| K1019 | Ubiquitination | Uniprot | |
| K1026 | Methylation | Uniprot | |
| K1026 | Ubiquitination | Uniprot | |
| S1028 | Phosphorylation | Uniprot | |
| R1031 | Methylation | Uniprot | |
| R1032 | Methylation | Uniprot | |
| R1042 | Methylation | Uniprot | |
| T1054 | Phosphorylation | Uniprot | |
| K1059 | Methylation | Uniprot | |
| K1059 | Ubiquitination | Uniprot | |
| T1066 | Phosphorylation | Uniprot | |
| K1071 | Ubiquitination | Uniprot | |
| T1073 | Phosphorylation | Uniprot | |
| K1074 | Ubiquitination | Uniprot | |
| S1077 | Phosphorylation | Uniprot | |
| S1080 | Phosphorylation | Uniprot | |
| S1092 | Phosphorylation | Uniprot | |
| K1095 | Acetylation | Uniprot | |
| K1095 | Methylation | Uniprot | |
| S1097 | Phosphorylation | Uniprot | |
| S1098 | Phosphorylation | Uniprot | |
| S1101 | Phosphorylation | Uniprot | |
| K1104 | Methylation | Uniprot | |
| S1106 | Phosphorylation | Uniprot | |
| S1108 | Phosphorylation | Uniprot | |
| S1110 | Phosphorylation | Uniprot | |
| T1117 | Phosphorylation | Uniprot | |
| T1119 | Phosphorylation | Uniprot | |
| R1138 | Methylation | Uniprot | |
| S1144 | Phosphorylation | Uniprot | |
| S1145 | Phosphorylation | Uniprot | |
| S1147 | Phosphorylation | P41279 (MAP3K8) | Uniprot |
| S1148 | Phosphorylation | Uniprot | |
| R1166 | Methylation | Uniprot | |
| T1179 | Phosphorylation | Uniprot | |
| S1185 | Phosphorylation | P17252 (PRKCA) | Uniprot |
| S1187 | Phosphorylation | Uniprot | |
| K1188 | Acetylation | Uniprot | |
| K1188 | Ubiquitination | Uniprot | |
| S1192 | Phosphorylation | Uniprot | |
| S1194 | Phosphorylation | Uniprot | |
| S1199 | Phosphorylation | Uniprot | |
| S1209 | Phosphorylation | Uniprot | |
| T1211 | Phosphorylation | Uniprot | |
| R1216 | Methylation | Uniprot | |
| S1231 | Phosphorylation | P06493 (CDK1) | Uniprot |
| K1234 | Ubiquitination | Uniprot | |
| S1238 | Phosphorylation | Uniprot | |
| K1244 | Acetylation | Uniprot | |
| K1244 | Ubiquitination | Uniprot | |
| Y1253 | Phosphorylation | Uniprot | |
| C1265 | S-Nitrosylation | Uniprot | |
| K1404 | Ubiquitination | Uniprot | |
| K1421 | Ubiquitination | Uniprot | |
| Y1424 | Phosphorylation | Uniprot | |
| T1425 | Phosphorylation | Uniprot | |
| S1430 | Phosphorylation | Uniprot | |
| R1436 | Methylation | Uniprot | |
| K1452 | Ubiquitination | Uniprot | |
| K1513 | Ubiquitination | Uniprot | |
| Y1526 | Phosphorylation | Uniprot | |
| T1534 | Phosphorylation | Uniprot | |
| Y1550 | Phosphorylation | Uniprot | |
| K1567 | Ubiquitination | Uniprot | |
| K1580 | Ubiquitination | Uniprot | |
| T1583 | Phosphorylation | Uniprot | |
| S1596 | Phosphorylation | Uniprot |
研究背景
Component of the protein complex eIF4F, which is involved in the recognition of the mRNA cap, ATP-dependent unwinding of 5'-terminal secondary structure and recruitment of mRNA to the ribosome.
Phosphorylated at multiple sites in vivo. Phosphorylation at Ser-1185 by PRKCA induces binding to MKNK1.
Following infection by certain enteroviruses, rhinoviruses and aphthoviruses, EIF4G1 is cleaved by the viral protease 2A, or the leader protease in the case of aphthoviruses. This shuts down the capped cellular mRNA transcription.
eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E (cap-binding) and EIF4G1/EIF4G3. Interacts with eIF3, mutually exclusive with EIF4A1 or EIFA2, EIF4E and through its N-terminus with PAPBC1. Interacts through its C-terminus with the serine/threonine kinases MKNK1, and with MKNK2. Appears to act as a scaffold protein, holding these enzymes in place to phosphorylate EIF4E. Non-phosphorylated EIF4EBP1 competes with EIF4G1/EIF4G3 to interact with EIF4E; insulin stimulated MAP-kinase (MAPK1 and MAPK3) phosphorylation of EIF4EBP1 causes dissociation of the complex allowing EIF4G1/EIF4G3 to bind and consequent initiation of translation. EIF4G1/EIF4G3 interacts with PABPC1 to bring about circularization of the mRNA. Rapamycin can attenuate insulin stimulation mediated by FKBPs. Interacts with EIF4E3. Interacts with CIRBP and MIF4GD. Interacts with RBM4. Interacts with HNRNPD/AUF1; the interaction requires RNA.
(Microbial infection) Interacts with rotavirus A NSP3; in this interaction, NSP3 takes the place of PABPC1 thereby inducing shutoff of host protein synthesis.
(Microbial infection) Interacts with human adenovirus 5 protein 100K; this interaction promotes translational shunt in presence of polysomes containing viral tripartite leader mRNAs.
Belongs to the eukaryotic initiation factor 4G family.
研究领域
· Genetic Information Processing > Translation > RNA transport.
· Human Diseases > Cardiovascular diseases > Viral myocarditis.
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