Phospho-EIF3B (Ser154) Antibody - #AF2352
| 产品: | 磷酸化 EIF3B (Ser154) 抗体 |
| 货号: | AF2352 |
| 描述: | Rabbit polyclonal antibody to Phospho-EIF3B (Ser154) |
| 应用: | WB IHC |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 116 kDa; 92kD(Calculated). |
| 蛋白号: | P55884 |
| RRID: | AB_2845366 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF2352, RRID:AB_2845366.
展开/折叠
eIF-3-eta; eIF3 eta; eIF3 p110; eIF3 p116; EIF3-ETA; EIF3-P110; EIF3-P116; eIF3b; EIF3B_HUMAN; EIF3S9; EIF3S9, formerly; Eukaryotic translation initiation factor 3, subunit B; Eukaryotic translation initiation factor 3 subunit 9; eukaryotic translation initiation factor 3 subunit 9 eta; Eukaryotic translation initiation factor 3 subunit B; eukaryotic translation initiation factor 3, subunit 9 (eta, 116kD); eukaryotic translation initiation factor 3, subunit 9 eta, 116kDa; eukaryotic translation initiation factor 3, subunit 9, formerly; hPrt1; Protein synthesis defective at 36 degrees celsius 1, S. cerevisiae, homolog of; PRT1; Prt1 homolog;
抗原和靶标
- P55884 EIF3B_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MQDAENVAVPEAAEERAEPGQQQPAAEPPPAEGLLRPAGPGAPEAAGTEASSEEVGIAEAGPESEVRTEPAAEAEAASGPSESPSPPAAEELPGSHAEPPVPAQGEAPGEQARDERSDSRAQAVSEDAGGNEGRAAEAEPRALENGDADEPSFSDPEDFVDDVSEEELLGDVLKDRPQEADGIDSVIVVDNVPQVGPDRLEKLKNVIHKIFSKFGKITNDFYPEEDGKTKGYIFLEYASPAHAVDAVKNADGYKLDKQHTFRVNLFTDFDKYMTISDEWDIPEKQPFKDLGNLRYWLEEAECRDQYSVIFESGDRTSIFWNDVKDPVSIEERARWTETYVRWSPKGTYLATFHQRGIALWGGEKFKQIQRFSHQGVQLIDFSPCERYLVTFSPLMDTQDDPQAIIIWDILTGHKKRGFHCESSAHWPIFKWSHDGKFFARMTLDTLSIYETPSMGLLDKKSLKISGIKDFSWSPGGNIIAFWVPEDKDIPARVTLMQLPTRQEIRVRNLFNVVDCKLHWQKNGDYLCVKVDRTPKGTQGVVTNFEIFRMREKQVPVDVVEMKETIIAFAWEPNGSKFAVLHGEAPRISVSFYHVKNNGKIELIKMFDKQQANTIFWSPQGQFVVLAGLRSMNGALAFVDTSDCTVMNIAEHYMASDVEWDPTGRYVVTSVSWWSHKVDNAYWLWTFQGRLLQKNNKDRFCQLLWRPRPPTLLSQEQIKQIKKDLKKYSKIFEQKDRLSQSKASKELVERRRTMMEDFRKYRKMAQELYMEQKNERLELRGGVDTDELDSNVDDWEEETIEFFVTEEIIPLGNQE
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P55884 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| T68 | Phosphorylation | Uniprot | |
| S78 | Phosphorylation | Uniprot | |
| S81 | Phosphorylation | Uniprot | |
| S83 | Phosphorylation | Uniprot | |
| S85 | Phosphorylation | Uniprot | |
| S95 | Phosphorylation | Uniprot | |
| S117 | Phosphorylation | Uniprot | |
| S119 | Phosphorylation | Uniprot | |
| S125 | Phosphorylation | Uniprot | |
| S152 | Phosphorylation | Uniprot | |
| S154 | Phosphorylation | Uniprot | |
| S164 | Phosphorylation | Uniprot | |
| K202 | Ubiquitination | Uniprot | |
| K204 | Ubiquitination | Uniprot | |
| K209 | Acetylation | Uniprot | |
| K209 | Ubiquitination | Uniprot | |
| K213 | Acetylation | Uniprot | |
| K213 | Ubiquitination | Uniprot | |
| K216 | Ubiquitination | Uniprot | |
| T218 | Phosphorylation | Uniprot | |
| K228 | Ubiquitination | Uniprot | |
| T229 | Phosphorylation | Uniprot | |
| K230 | Sumoylation | Uniprot | |
| K230 | Ubiquitination | Uniprot | |
| Y232 | Phosphorylation | Uniprot | |
| Y237 | Phosphorylation | Uniprot | |
| S239 | Phosphorylation | Uniprot | |
| K248 | Ubiquitination | Uniprot | |
| Y253 | Phosphorylation | Uniprot | |
| K254 | Ubiquitination | Uniprot | |
| K257 | Ubiquitination | Uniprot | |
| T267 | Phosphorylation | Uniprot | |
| Y272 | Phosphorylation | Uniprot | |
| K284 | Ubiquitination | Uniprot | |
| K288 | Acetylation | Uniprot | |
| K288 | Ubiquitination | Uniprot | |
| S312 | Phosphorylation | Uniprot | |
| K324 | Ubiquitination | Uniprot | |
| Y339 | Phosphorylation | Uniprot | |
| K345 | Ubiquitination | Uniprot | |
| K364 | Acetylation | Uniprot | |
| K364 | Ubiquitination | Uniprot | |
| T390 | Phosphorylation | Uniprot | |
| S392 | Phosphorylation | Uniprot | |
| T397 | Phosphorylation | Uniprot | |
| R416 | Methylation | Uniprot | |
| K436 | Acetylation | Uniprot | |
| S447 | Phosphorylation | Uniprot | |
| Y449 | Phosphorylation | Uniprot | |
| K459 | Ubiquitination | Uniprot | |
| K460 | Ubiquitination | Uniprot | |
| S461 | Phosphorylation | Uniprot | |
| K463 | Ubiquitination | Uniprot | |
| S465 | Phosphorylation | Uniprot | |
| S473 | Phosphorylation | Uniprot | |
| K487 | Ubiquitination | Uniprot | |
| T500 | Phosphorylation | Uniprot | |
| K516 | Ubiquitination | Uniprot | |
| K521 | Ubiquitination | Uniprot | |
| Y525 | Phosphorylation | Uniprot | |
| K529 | Ubiquitination | Uniprot | |
| K535 | Ubiquitination | Uniprot | |
| T537 | Phosphorylation | Uniprot | |
| K552 | Ubiquitination | Uniprot | |
| S575 | Phosphorylation | Uniprot | |
| K599 | Ubiquitination | Uniprot | |
| K604 | Ubiquitination | Uniprot | |
| K608 | Ubiquitination | Uniprot | |
| K693 | Ubiquitination | Uniprot | |
| T710 | Phosphorylation | Uniprot | |
| S713 | Phosphorylation | Uniprot | |
| K718 | Ubiquitination | Uniprot | |
| K726 | Ubiquitination | Uniprot | |
| S728 | Phosphorylation | Uniprot | |
| K729 | Acetylation | Uniprot | |
| K729 | Ubiquitination | Uniprot | |
| K734 | Ubiquitination | Uniprot | |
| S738 | Phosphorylation | Uniprot | |
| K741 | Ubiquitination | Uniprot | |
| K744 | Acetylation | Uniprot | |
| K744 | Ubiquitination | Uniprot | |
| T752 | Phosphorylation | Uniprot | |
| R758 | Methylation | Uniprot | |
| K762 | Ubiquitination | Uniprot | |
| Y768 | Phosphorylation | Uniprot | |
| K772 | Ubiquitination | Uniprot |
研究背景
RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression.
(Microbial infection) In case of FCV infection, plays a role in the ribosomal termination-reinitiation event leading to the translation of VP2.
Phosphorylated. Phosphorylation is enhanced upon serum stimulation.
Cytoplasm.
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L and EIF3M. The eIF-3 complex appears to include 3 stable modules: module A is composed of EIF3A, EIF3B, EIF3G and EIF3I; module B is composed of EIF3F, EIF3H, and EIF3M; and module C is composed of EIF3C, EIF3D, EIF3E, EIF3K and EIF3L. EIF3C of module C binds EIF3B of module A and EIF3H of module B, thereby linking the three modules. EIF3J is a labile subunit that binds to the eIF-3 complex via EIF3B. The eIF-3 complex interacts with RPS6KB1 under conditions of nutrient depletion. Mitogenic stimulation leads to binding and activation of a complex composed of MTOR and RPTOR, leading to phosphorylation and release of RPS6KB1 and binding of EIF4B to eIF-3. Also interacts with UPF2 and HNRPD. Interacts with METTL3.
The RRM domain mediates interaction with EIF3J.
Belongs to the eIF-3 subunit B family.
研究领域
· Genetic Information Processing > Translation > RNA transport.
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