Phospho-Cytokeratin 20 (Ser13) Antibody - #AF2356
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF2356, RRID:AB_2845370.
展开/折叠
CD20; CK 20; CK-20; CK20; Cytokeratin-20; Cytokeratin20; K1C20_HUMAN; K20; KA20; Keratin 20; keratin 20, type I; keratin 21, rat, homolog of; Keratin; Keratin type I cytoskeletal 20; Keratin-20; Keratin20; KRT 20; KRT 21; KRT20; KRT21; MGC35423; OTTHUMP00000164518; Protein IT; type I cytoskeletal 20;
抗原和靶标
Expressed predominantly in the intestinal epithelium. Expressed in luminal cells of colonic mucosa. Also expressed in the Merkel cells of keratinized oral mucosa; specifically at the tips of some rete ridges of the gingival mucosa, in the basal layer of the palatal mucosa and in the taste buds of lingual mucosa.
- P35900 K1C20_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDFSRRSFHRSLSSSLQAPVVSTVGMQRLGTTPSVYGGAGGRGIRISNSRHTVNYGSDLTGGGDLFVGNEKMAMQNLNDRLASYLEKVRTLEQSNSKLEVQIKQWYETNAPRAGRDYSAYYRQIEELRSQIKDAQLQNARCVLQIDNAKLAAEDFRLKYETERGIRLTVEADLQGLNKVFDDLTLHKTDLEIQIEELNKDLALLKKEHQEEVDGLHKHLGNTVNVEVDAAPGLNLGVIMNEMRQKYEVMAQKNLQEAKEQFERQTAVLQQQVTVNTEELKGTEVQLTELRRTSQSLEIELQSHLSMKESLEHTLEETKARYSSQLANLQSLLSSLEAQLMQIRSNMERQNNEYHILLDIKTRLEQEIATYRRLLEGEDVKTTEYQLSTLEERDIKKTRKIKTVVQEVVDGKVVSSEVKEVEENI
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P35900 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S11 | Phosphorylation | Uniprot | |
| S13 | Phosphorylation | P49137 (MAPKAPK2) , P17252 (PRKCA) | Uniprot |
| S34 | Phosphorylation | Uniprot | |
| Y36 | Phosphorylation | Uniprot | |
| R42 | Methylation | Uniprot | |
| Y84 | Phosphorylation | Uniprot | |
| S94 | Phosphorylation | Uniprot | |
| S118 | Phosphorylation | Uniprot | |
| Y120 | Phosphorylation | Uniprot | |
| Y121 | Phosphorylation | Uniprot | |
| K149 | Ubiquitination | Uniprot | |
| T168 | Phosphorylation | Uniprot | |
| T369 | Phosphorylation | Uniprot | |
| Y370 | Phosphorylation | Uniprot | |
| Y384 | Phosphorylation | Uniprot | |
| S387 | Phosphorylation | Uniprot | |
| T402 | Phosphorylation | Uniprot |
研究背景
Plays a significant role in maintaining keratin filament organization in intestinal epithelia. When phosphorylated, plays a role in the secretion of mucin in the small intestine (By similarity).
Hyperphosphorylation at Ser-13 occurs during the early stages of apoptosis but becomes less prominent during the later stages. Phosphorylation at Ser-13 also increases in response to stress brought on by cell injury (By similarity).
Proteolytically cleaved by caspases during apoptosis. Cleavage occurs at Asp-228.
Cytoplasm.
Expressed predominantly in the intestinal epithelium. Expressed in luminal cells of colonic mucosa. Also expressed in the Merkel cells of keratinized oral mucosa; specifically at the tips of some rete ridges of the gingival mucosa, in the basal layer of the palatal mucosa and in the taste buds of lingual mucosa.
Heterotetramer of two type I and two type II keratins. Associates with KRT8.
Belongs to the intermediate filament family.
研究领域
· Organismal Systems > Endocrine system > Estrogen signaling pathway. (View pathway)
限制条款
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