Phospho-MCM3 (Ser112) Antibody - #AF2362
| 产品: | 磷酸化 MCM3 (Ser112) 抗体 |
| 货号: | AF2362 |
| 描述: | Rabbit polyclonal antibody to Phospho-MCM3 (Ser112) |
| 应用: | WB IHC |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 100kDa; 91kD(Calculated). |
| 蛋白号: | P25205 |
| RRID: | AB_2845376 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF2362, RRID:AB_2845376.
展开/折叠
Cervical cancer proto oncogene 5; DNA polymerase alpha holoenzyme associated P1; DNA polymerase alpha holoenzyme associated protein P1; DNA polymerase alpha holoenzyme-associated protein P1; DNA replication factor MCM3; DNA replication licensing factor mcm3; HCC 5; HCC5; hRlf beta subunit; Human cervical cancer proto oncogene 5; MCM 3; mcm3; MCM3 minichromosome maintenance deficient 3; MCM3_HUMAN; MGC1157; Minichromosome maintenance complex component 3; Minichromosome maintenance deficient 3; Minichromosome maintenance protein 3; P1 h; P1 MCM3; P1 Protein; P1-MCM3; P1.h; p102; P102 protein; Replication licensing factor beta subunit; RLF beta subunit; RLF subunit beta; RLFB;
抗原和靶标
- P25205 MCM3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAGTVVLDDVELREAQRDYLDFLDDEEDQGIYQSKVRELISDNQYRLIVNVNDLRRKNEKRANRLLNNAFEELVAFQRALKDFVASIDATYAKQYEEFYVGLEGSFGSKHVSPRTLTSCFLSCVVCVEGIVTKCSLVRPKVVRSVHYCPATKKTIERRYSDLTTLVAFPSSSVYPTKDEENNPLETEYGLSVYKDHQTITIQEMPEKAPAGQLPRSVDVILDDDLVDKAKPGDRVQVVGTYRCLPGKKGGYTSGTFRTVLIACNVKQMSKDAQPSFSAEDIAKIKKFSKTRSKDIFDQLAKSLAPSIHGHDYVKKAILCLLLGGVERDLENGSHIRGDINILLIGDPSVAKSQLLRYVLCTAPRAIPTTGRGSSGVGLTAAVTTDQETGERRLEAGAMVLADRGVVCIDEFDKMSDMDRTAIHEVMEQGRVTIAKAGIHARLNARCSVLAAANPVYGRYDQYKTPMENIGLQDSLLSRFDLLFIMLDQMDPEQDREISDHVLRMHRYRAPGEQDGDAMPLGSAVDILATDDPNFSQEDQQDTQIYEKHDNLLHGTKKKKEKMVSAAFMKKYIHVAKIIKPVLTQESATYIAEEYSRLRSQDSMSSDTARTSPVTARTLETLIRLATAHAKARMSKTVDLQDAEEAVELVQYAYFKKVLEKEKKRKKRSEDESETEDEEEKSQEDQEQKRKRRKTRQPDAKDGDSYDPYDFSDTEEEMPQVHTPKTADSQETKESQKVELSESRLKAFKVALLDVFREAHAQSIGMNRLTESINRDSEEPFSSVEIQAALSKMQDDNQVMVSEGIIFLI
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P25205 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| T4 | Phosphorylation | Uniprot | |
| Y19 | Phosphorylation | Uniprot | |
| Y32 | Phosphorylation | Uniprot | |
| S34 | Phosphorylation | Uniprot | |
| K35 | Ubiquitination | Uniprot | |
| Y45 | Phosphorylation | Uniprot | |
| R55 | Methylation | Uniprot | |
| K81 | Ubiquitination | Uniprot | |
| S112 | Phosphorylation | P06493 (CDK1) | Uniprot |
| K140 | Ubiquitination | Uniprot | |
| S144 | Phosphorylation | Uniprot | |
| C148 | S-Nitrosylation | Uniprot | |
| K152 | Acetylation | Uniprot | |
| K152 | Ubiquitination | Uniprot | |
| T154 | Phosphorylation | Uniprot | |
| Y159 | Phosphorylation | Uniprot | |
| S160 | Phosphorylation | P53355 (DAPK1) , O14757 (CHEK1) | Uniprot |
| T163 | Phosphorylation | Uniprot | |
| T164 | Phosphorylation | Uniprot | |
| S170 | Phosphorylation | Uniprot | |
| S171 | Phosphorylation | Uniprot | |
| T176 | Phosphorylation | Uniprot | |
| K177 | Ubiquitination | Uniprot | |
| T198 | Phosphorylation | Uniprot | |
| K207 | Ubiquitination | Uniprot | |
| K228 | Ubiquitination | Uniprot | |
| K230 | Ubiquitination | Uniprot | |
| R234 | Methylation | Uniprot | |
| T240 | Phosphorylation | Uniprot | |
| K248 | Sumoylation | Uniprot | |
| K248 | Ubiquitination | Uniprot | |
| T252 | Phosphorylation | Uniprot | |
| T255 | Phosphorylation | Uniprot | |
| T258 | Phosphorylation | Uniprot | |
| C263 | S-Nitrosylation | Uniprot | |
| K266 | Sumoylation | Uniprot | |
| K266 | Ubiquitination | Uniprot | |
| K270 | Ubiquitination | Uniprot | |
| S275 | Phosphorylation | Uniprot | |
| K283 | Ubiquitination | Uniprot | |
| S292 | Phosphorylation | Uniprot | |
| K293 | Ubiquitination | Uniprot | |
| K301 | Ubiquitination | Uniprot | |
| Y312 | Phosphorylation | Uniprot | |
| K314 | Ubiquitination | Uniprot | |
| K315 | Ubiquitination | Uniprot | |
| K351 | Ubiquitination | Uniprot | |
| R371 | Methylation | Uniprot | |
| T383 | Phosphorylation | Uniprot | |
| T384 | Phosphorylation | Uniprot | |
| K413 | Ubiquitination | Uniprot | |
| R419 | Methylation | Uniprot | |
| K435 | Ubiquitination | Uniprot | |
| K463 | Ubiquitination | Uniprot | |
| S474 | Phosphorylation | Uniprot | |
| S535 | Phosphorylation | Q13535 (ATR) , Q13315 (ATM) | Uniprot |
| Y545 | Phosphorylation | Uniprot | |
| T555 | Phosphorylation | Uniprot | |
| K556 | Acetylation | Uniprot | |
| K556 | Ubiquitination | Uniprot | |
| K559 | Acetylation | Uniprot | |
| K561 | Ubiquitination | Uniprot | |
| K570 | Ubiquitination | Uniprot | |
| K579 | Ubiquitination | Uniprot | |
| S599 | Phosphorylation | Uniprot | |
| S611 | Phosphorylation | Uniprot | |
| T617 | Phosphorylation | Uniprot | |
| K635 | Ubiquitination | Uniprot | |
| Y651 | Phosphorylation | Uniprot | |
| K655 | Ubiquitination | Uniprot | |
| S668 | Phosphorylation | Uniprot | |
| S672 | Phosphorylation | Uniprot | |
| T674 | Phosphorylation | Uniprot | |
| S681 | Phosphorylation | Uniprot | |
| K688 | Ubiquitination | Uniprot | |
| S704 | Phosphorylation | Uniprot | |
| Y705 | Phosphorylation | Uniprot | |
| Y708 | Phosphorylation | Uniprot | |
| S711 | Phosphorylation | Uniprot | |
| T713 | Phosphorylation | Uniprot | |
| T722 | Phosphorylation | P24941 (CDK2) | Uniprot |
| T725 | Phosphorylation | Uniprot | |
| S728 | Phosphorylation | Q13315 (ATM) | Uniprot |
| K732 | Sumoylation | Uniprot | |
| K732 | Ubiquitination | Uniprot | |
| S734 | Phosphorylation | Uniprot | |
| K736 | Sumoylation | Uniprot | |
| K736 | Ubiquitination | Uniprot | |
| S740 | Phosphorylation | Uniprot | |
| S742 | Phosphorylation | Uniprot | |
| R743 | Methylation | Uniprot | |
| K748 | Ubiquitination | Uniprot |
研究背景
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for DNA replication and cell proliferation.
Acetylated by MCM3AP.
O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.
Nucleus.
Component of the MCM2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order MCM2-MCM6-MCM4-MCM7-MCM3-MCM5 (Probable). Associated with the replication-specific DNA polymerase alpha. Interacts with MCMBP. Interacts with ANKRD17. Interacts with MCM3AP isoform MCM3AP; this interaction leads to MCM3 acetylation.
Belongs to the MCM family.
研究领域
· Cellular Processes > Cell growth and death > Cell cycle. (View pathway)
· Genetic Information Processing > Replication and repair > DNA replication.
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