| 产品: | 磷酸化 Myt1 (Ser83) 抗体 |
| 货号: | AF2369 |
| 描述: | Rabbit polyclonal antibody to Phospho-Myt1 (Ser83) |
| 应用: | WB IHC |
| 反应: | Human |
| 预测: | Pig, Bovine, Horse, Rabbit, Dog |
| 分子量: | 70kDa; 55kD(Calculated). |
| 蛋白号: | Q99640 |
| RRID: | AB_2845383 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF2369, RRID:AB_2845383.
展开/折叠
cdc2 inhibitory kinase; DKFZp547K1610; FLJ20093; Membrane associated tyrosine and threonine specific cdc2 inhibitory kinase; Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase; MYT1; Myt1 kinase; PKMYT 1; Pkmyt1; PMYT1_HUMAN; PPP1R126; Protein kinase membrane associated tyrosine/threonine 1; Protein kinase Myt1; Protein phosphatase 1 regulatory subunit 126;
抗原和靶标
- Q99640 PMYT1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MLERPPALAMPMPTEGTPPPLSGTPIPVPAYFRHAEPGFSLKRPRGLSRSLPPPPPAKGSIPISRLFPPRTPGWHQLQPRRVSFRGEASETLQSPGYDPSRPESFFQQSFQRLSRLGHGSYGEVFKVRSKEDGRLYAVKRSMSPFRGPKDRARKLAEVGSHEKVGQHPCCVRLEQAWEEGGILYLQTELCGPSLQQHCEAWGASLPEAQVWGYLRDTLLALAHLHSQGLVHLDVKPANIFLGPRGRCKLGDFGLLVELGTAGAGEVQEGDPRYMAPELLQGSYGTAADVFSLGLTILEVACNMELPHGGEGWQQLRQGYLPPEFTAGLSSELRSVLVMMLEPDPKLRATAEALLALPVLRQPRAWGVLWCMAAEALSRGWALWQALLALLCWLWHGLAHPASWLQPLGPPATPPGSPPCSLLLDSSLSSNWDDDSLGPSLSPEAVLARTVGSTSTPRSRCTPRDALDLSDINSEPPRGSFPSFEPRNLLSLFEDTLDPT
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q99640 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Phosphorylation | Uniprot | ||
| M1 | Acetylation | Uniprot | |
| T17 | Phosphorylation | Uniprot | |
| T24 | Phosphorylation | Uniprot | |
| S40 | Phosphorylation | Uniprot | |
| K42 | Methylation | Uniprot | |
| K42 | Ubiquitination | Uniprot | |
| S48 | Phosphorylation | Uniprot | |
| S50 | Phosphorylation | Uniprot | |
| K58 | Ubiquitination | Uniprot | |
| S60 | Phosphorylation | Uniprot | |
| S64 | Phosphorylation | Uniprot | |
| T71 | Phosphorylation | Uniprot | |
| S83 | Phosphorylation | P31749 (AKT1) , P45983 (MAPK8) | Uniprot |
| S89 | Phosphorylation | Uniprot | |
| S94 | Phosphorylation | Uniprot | |
| Y97 | Phosphorylation | Uniprot | |
| S109 | Phosphorylation | Uniprot | |
| S120 | Phosphorylation | Uniprot | |
| K126 | Methylation | Uniprot | |
| K126 | Ubiquitination | Uniprot | |
| K139 | Ubiquitination | Uniprot | |
| S141 | Phosphorylation | Uniprot | |
| S143 | Phosphorylation | Uniprot | |
| K149 | Ubiquitination | Uniprot | |
| K154 | Ubiquitination | Uniprot | |
| S160 | Phosphorylation | Uniprot | |
| K163 | Ubiquitination | Uniprot | |
| K345 | Ubiquitination | Uniprot | |
| T412 | Phosphorylation | Uniprot | |
| S416 | Phosphorylation | Uniprot | |
| S426 | Phosphorylation | P53350 (PLK1) | Uniprot |
| S441 | Phosphorylation | Uniprot | |
| T453 | Phosphorylation | Uniprot | |
| S454 | Phosphorylation | Uniprot | |
| T455 | Phosphorylation | Uniprot | |
| S458 | Phosphorylation | Uniprot | |
| T461 | Phosphorylation | Uniprot | |
| S469 | Phosphorylation | Uniprot | |
| S473 | Phosphorylation | Uniprot | |
| S479 | Phosphorylation | Uniprot | |
| S482 | Phosphorylation | Uniprot | |
| T495 | Phosphorylation | P53350 (PLK1) | Uniprot |
研究背景
Acts as a negative regulator of entry into mitosis (G2 to M transition) by phosphorylation of the CDK1 kinase specifically when CDK1 is complexed to cyclins. Mediates phosphorylation of CDK1 predominantly on 'Thr-14'. Also involved in Golgi fragmentation. May be involved in phosphorylation of CDK1 on 'Tyr-15' to a lesser degree, however tyrosine kinase activity is unclear and may be indirect. May be a downstream target of Notch signaling pathway during eye development.
Autophosphorylated. Phosphorylated by CDC2-CCNB1 complexes on undefined serine and threonine residues. The phosphorylation by CDC2-CCNB1 complexes may inhibit the catalytic activity.
Endoplasmic reticulum membrane>Peripheral membrane protein. Golgi apparatus membrane>Peripheral membrane protein.
Interacts with CDC2-CCNB1 complex. Can also interact with PIN1 when phosphorylated by CDC2-CCNB1.
The membrane-association motif is essential for the localization to membrane of Golgi stack. According to some authors, it is a transmembrane domain; the existence of a transmembrane region is however unproven.
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WEE1 subfamily.
研究领域
· Cellular Processes > Cell growth and death > Cell cycle. (View pathway)
· Cellular Processes > Cell growth and death > Oocyte meiosis. (View pathway)
· Organismal Systems > Endocrine system > Progesterone-mediated oocyte maturation.
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