| 产品: | 磷酸化 NEDD4L (Ser448) 抗体 |
| 货号: | AF2370 |
| 描述: | Rabbit polyclonal antibody to Phospho-NEDD4L (Ser448) |
| 应用: | WB IHC |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Zebrafish, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 110, 135kDa; 112kD(Calculated). |
| 蛋白号: | Q96PU5 |
| RRID: | AB_2845384 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF2370, RRID:AB_2845384.
展开/折叠
E3 ubiquitin protein ligase NEDD4 like protein; E3 ubiquitin-protein ligase NEDD4-like; KIAA0439; NED4L_HUMAN; NEDD4 2; NEDD4 2c; Nedd4-2; NEDD4-2a; NEDD4.2; NEDD4b; NEDD4L; NEDD4La; NEDD4Lb; NEDD4Lc; NEDD4Ld; NEDD4Le; NEDD4Lf; NEDD4Lg; NEDD4Lh; NEDL3; Neural precursor cell expressed developmentally down regulated 4 like E3 ubiquitin protein ligase; Neural precursor cell expressed, developmentally down regulated 4 like; RSP5; Ubiquitin protein ligase Rsp5;
抗原和靶标
Ubiquitously expressed, with highest levels in prostate, pancreas and kidney (PubMed:14615060, PubMed:15496141, PubMed:19664597). Expressed in melanocytes (PubMed:23999003).
- Q96PU5 NED4L_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MATGLGEPVYGLSEDEGESRILRVKVVSGIDLAKKDIFGASDPYVKLSLYVADENRELALVQTKTIKKTLNPKWNEEFYFRVNPSNHRLLFEVFDENRLTRDDFLGQVDVPLSHLPTEDPTMERPYTFKDFLLRPRSHKSRVKGFLRLKMAYMPKNGGQDEENSDQRDDMEHGWEVVDSNDSASQHQEELPPPPLPPGWEEKVDNLGRTYYVNHNNRTTQWHRPSLMDVSSESDNNIRQINQEAAHRRFRSRRHISEDLEPEPSEGGDVPEPWETISEEVNIAGDSLGLALPPPPASPGSRTSPQELSEELSRRLQITPDSNGEQFSSLIQREPSSRLRSCSVTDAVAEQGHLPPPSAPAGRARSSTVTGGEEPTPSVAYVHTTPGLPSGWEERKDAKGRTYYVNHNNRTTTWTRPIMQLAEDGASGSATNSNNHLIEPQIRRPRSLSSPTVTLSAPLEGAKDSPVRRAVKDTLSNPQSPQPSPYNSPKPQHKVTQSFLPPGWEMRIAPNGRPFFIDHNTKTTTWEDPRLKFPVHMRSKTSLNPNDLGPLPPGWEERIHLDGRTFYIDHNSKITQWEDPRLQNPAITGPAVPYSREFKQKYDYFRKKLKKPADIPNRFEMKLHRNNIFEESYRRIMSVKRPDVLKARLWIEFESEKGLDYGGVAREWFFLLSKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFTFIGRVAGLAVFHGKLLDGFFIRPFYKMMLGKQITLNDMESVDSEYYNSLKWILENDPTELDLMFCIDEENFGQTYQVDLKPNGSEIMVTNENKREYIDLVIQWRFVNRVQKQMNAFLEGFTELLPIDLIKIFDENELELLMCGLGDVDVNDWRQHSIYKNGYCPNHPVIQWFWKAVLLMDAEKRIRLLQFVTGTSRVPMNGFAELYGSNGPQLFTIEQWGSPEKLPRAHTCFNRLDLPPYETFEDLREKLLMAVENAQGFEGVD
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q96PU5 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| S13 | Phosphorylation | Uniprot | |
| K25 | Ubiquitination | Uniprot | |
| S28 | Phosphorylation | Uniprot | |
| K129 | Ubiquitination | Uniprot | |
| K149 | Ubiquitination | Uniprot | |
| K155 | Ubiquitination | Uniprot | |
| S225 | Phosphorylation | Uniprot | |
| S297 | Phosphorylation | Uniprot | |
| S300 | Phosphorylation | Uniprot | |
| T302 | Phosphorylation | Uniprot | |
| S303 | Phosphorylation | Uniprot | |
| S312 | Phosphorylation | Uniprot | |
| T318 | Phosphorylation | Uniprot | |
| S321 | Phosphorylation | Uniprot | |
| S327 | Phosphorylation | Uniprot | |
| S328 | Phosphorylation | Uniprot | |
| S335 | Phosphorylation | Uniprot | |
| S336 | Phosphorylation | Uniprot | |
| S340 | Phosphorylation | Uniprot | |
| S342 | Phosphorylation | P17612 (PRKACA) , O00141 (SGK1) | Uniprot |
| T344 | Phosphorylation | Uniprot | |
| S357 | Phosphorylation | Uniprot | |
| S365 | Phosphorylation | Uniprot | |
| S366 | Phosphorylation | Uniprot | |
| T367 | Phosphorylation | P17612 (PRKACA) , O00141 (SGK1) | Uniprot |
| T369 | Phosphorylation | Uniprot | |
| T375 | Phosphorylation | Uniprot | |
| S377 | Phosphorylation | Uniprot | |
| Y380 | Phosphorylation | Uniprot | |
| T383 | Phosphorylation | Uniprot | |
| S428 | Phosphorylation | Uniprot | |
| S446 | Phosphorylation | Uniprot | |
| S448 | Phosphorylation | P17612 (PRKACA) , O00141 (SGK1) , Q96BR1 (SGK3) | Uniprot |
| S449 | Phosphorylation | Uniprot | |
| T451 | Phosphorylation | Uniprot | |
| T453 | Phosphorylation | Uniprot | |
| K462 | Ubiquitination | Uniprot | |
| S464 | Phosphorylation | Uniprot | |
| K471 | Ubiquitination | Uniprot | |
| T473 | Phosphorylation | Uniprot | |
| S475 | Phosphorylation | Uniprot | |
| S479 | Phosphorylation | Uniprot | |
| S483 | Phosphorylation | Uniprot | |
| Y485 | Phosphorylation | Uniprot | |
| S487 | Phosphorylation | Uniprot | |
| K489 | Ubiquitination | Uniprot | |
| K493 | Ubiquitination | Uniprot | |
| K521 | Ubiquitination | Uniprot | |
| K531 | Ubiquitination | Uniprot | |
| S538 | Phosphorylation | Uniprot | |
| K539 | Ubiquitination | Uniprot | |
| S571 | Phosphorylation | Uniprot | |
| K572 | Ubiquitination | Uniprot | |
| K639 | Ubiquitination | Uniprot | |
| K656 | Ubiquitination | Uniprot | |
| Y660 | Phosphorylation | Uniprot | |
| S795 | Phosphorylation | Uniprot | |
| T903 | Phosphorylation | Uniprot |
研究背景
E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Inhibits TGF-beta signaling by triggering SMAD2 and TGFBR1 ubiquitination and proteasome-dependent degradation. Promotes ubiquitination and internalization of various plasma membrane channels such as ENaC, SCN2A/Nav1.2, SCN3A/Nav1.3, SCN5A/Nav1.5, SCN9A/Nav1.7, SCN10A/Nav1.8, KCNA3/Kv1.3, KCNH2, EAAT1, KCNQ2/Kv7.2, KCNQ3/Kv7.3 or CLC5. Promotes ubiquitination and degradation of SGK1 and TNK2. Ubiquitinates BRAT1 and this ubiquitination is enhanced in the presence of NDFIP1. Plays a role in dendrite formation by melanocytes. Involved in the regulation of TOR signaling. Ubiquitinates and regulates protein levels of NTRK1 once this one is activated by NGF.
Phosphorylated by SGK1 or PKA; which impairs interaction with SCNN. Interaction with YWHAH inhibits dephosphorylation.
Auto-ubiquitinated. Deubiquitinated by USP36, no effect on NEDD4L protein levels. Both proteins interact and regulate each other's ubiquitination levels.
Cytoplasm. Golgi apparatus. Endosome>Multivesicular body.
Note: May be recruited to exosomes by NDFIP1.
Ubiquitously expressed, with highest levels in prostate, pancreas and kidney. Expressed in melanocytes.
Interacts with UBE2E3 (By similarity). Interacts with NDFIP1 and NDFIP2; this interaction activates the E3 ubiquitin-protein ligase. Interacts via its WW domains with SCNN1A, SCNN1B, SCNN1G, SCN1A, SCN2A, SCN3A, SCN5A, SCN8A, SCN9A, SCN10A and CLCN5. Interacts with SMAD2, SMAD3, SMAD6 and SMAD7. The phosphorylated form interacts with 14-3-3 proteins. Interacts with TNK2. Interacts with WNK1. Interacts with SGK1. Interacts (via C2 domain) with NPC2. Interacts with ARRDC4. Interacts with KCNQ1; promotes internalization of KCNQ1. Interacts (via domains WW1, 3 and 4) with USP36; the interaction inhibits ubiquitination of, at least, NTRK1, KCNQ2 and KCNQ3 by NEDD4L.
(Microbial infection) Interacts with Epstein-Barr virus LMP2A.
研究领域
· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)
· Cellular Processes > Cellular community - eukaryotes > Tight junction. (View pathway)
· Genetic Information Processing > Folding, sorting and degradation > Ubiquitin mediated proteolysis. (View pathway)
· Organismal Systems > Excretory system > Aldosterone-regulated sodium reabsorption.
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