Phospho-p40 phox (Thr154) Antibody - #AF2376
| 产品: | 磷酸化 p40 phox (Thr154) 抗体 |
| 货号: | AF2376 |
| 描述: | Rabbit polyclonal antibody to Phospho-p40 phox (Thr154) |
| 应用: | WB IHC |
| 反应: | Human, Mouse |
| 预测: | Pig, Zebrafish, Horse, Rabbit, Dog, Xenopus |
| 分子量: | 40kDa; 39kD(Calculated). |
| 蛋白号: | Q15080 |
| RRID: | AB_2845390 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF2376, RRID:AB_2845390.
展开/折叠
CGD3; MGC3810; NCF 4; NCF; NCF-4; Ncf4; NCF4_HUMAN; Neutrophil cytosol factor 4; Neutrophil cytosolic factor 4; Neutrophil NADPH oxidase factor 4; p40-phox; p40phox; SH3 and PX domain-containing protein 4; SH3PXD4;
抗原和靶标
- Q15080 NCF4_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAVAQQLRAESDFEQLPDDVAISANIADIEEKRGFTSHFVFVIEVKTKGGSKYLIYRRYRQFHALQSKLEERFGPDSKSSALACTLPTLPAKVYVGVKQEIAEMRIPALNAYMKSLLSLPVWVLMDEDVRIFFYQSPYDSEQVPQALRRLRPRTRKVKSVSPQGNSVDRMAAPRAEALFDFTGNSKLELNFKAGDVIFLLSRINKDWLEGTVRGATGIFPLSFVKILKDFPEEDDPTNWLRCYYYEDTISTIKDIAVEEDLSSTPLLKDLLELTRREFQREDIALNYRDAEGDLVRLLSDEDVALMVRQARGLPSQKRLFPWKLHITQKDNYRVYNTMP
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q15080 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S11 | Phosphorylation | Uniprot | |
| S23 | Phosphorylation | Uniprot | |
| T47 | Phosphorylation | Uniprot | |
| S51 | Phosphorylation | Uniprot | |
| Y53 | Phosphorylation | Uniprot | |
| T154 | Phosphorylation | Q05655 (PRKCD) , P17252 (PRKCA) | Uniprot |
| S161 | Phosphorylation | Uniprot | |
| S166 | Phosphorylation | Uniprot | |
| K228 | Ubiquitination | Uniprot | |
| Y243 | Phosphorylation | Uniprot | |
| Y245 | Phosphorylation | Uniprot | |
| S299 | Phosphorylation | Uniprot | |
| S315 | Phosphorylation | P17252 (PRKCA) | Uniprot |
研究背景
Component of the NADPH-oxidase, a multicomponent enzyme system responsible for the oxidative burst in which electrons are transported from NADPH to molecular oxygen, generating reactive oxidant intermediates. It may be important for the assembly and/or activation of the NADPH-oxidase complex.
Cytoplasm>Cytosol. Endosome membrane>Peripheral membrane protein>Cytoplasmic side. Membrane>Peripheral membrane protein.
Expression is restricted to hematopoietic cells.
Component of an NADPH oxidase complex composed of a heterodimer formed by the membrane proteins CYBA and CYBB and the cytosolic subunits NCF1, NCF2 and NCF4. NCF4 interacts primarily with NCF2 to form a complex with NCF1.
The PB1 domain mediates the association with NCF2/p67-PHOX.
The PX domain mediates interaction with membranes enriched in phosphatidylnositol 3-phosphate.
研究领域
· Cellular Processes > Transport and catabolism > Phagosome. (View pathway)
· Human Diseases > Infectious diseases: Parasitic > Leishmaniasis.
· Organismal Systems > Development > Osteoclast differentiation. (View pathway)
· Organismal Systems > Immune system > Leukocyte transendothelial migration. (View pathway)
限制条款
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