产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF2401, RRID:AB_2845415.
展开/折叠
Hsh 155; MDS; Pre mRNA processing 10; Pre mRNA splicing factor SF3b 155 kDa subunit; PRP 10; PRP10; PRPF 10; PRPF10; SAP 155; SAP155; SF3B 1; SF3b155; Spliceosome associated protein 155; Splicing factor 3b subunit 1 155kDa; Splicing factor 3B subunit 1;
抗原和靶标
- O75533 SF3B1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAKIAKTHEDIEAQIREIQGKKAALDEAQGVGLDSTGYYDQEIYGGSDSRFAGYVTSIAATELEDDDDDYSSSTSLLGQKKPGYHAPVALLNDIPQSTEQYDPFAEHRPPKIADREDEYKKHRRTMIISPERLDPFADGGKTPDPKMNARTYMDVMREQHLTKEEREIRQQLAEKAKAGELKVVNGAAASQPPSKRKRRWDQTADQTPGATPKKLSSWDQAETPGHTPSLRWDETPGRAKGSETPGATPGSKIWDPTPSHTPAGAATPGRGDTPGHATPGHGGATSSARKNRWDETPKTERDTPGHGSGWAETPRTDRGGDSIGETPTPGASKRKSRWDETPASQMGGSTPVLTPGKTPIGTPAMNMATPTPGHIMSMTPEQLQAWRWEREIDERNRPLSDEELDAMFPEGYKVLPPPAGYVPIRTPARKLTATPTPLGGMTGFHMQTEDRTMKSVNDQPSGNLPFLKPDDIQYFDKLLVDVDESTLSPEEQKERKIMKLLLKIKNGTPPMRKAALRQITDKAREFGAGPLFNQILPLLMSPTLEDQERHLLVKVIDRILYKLDDLVRPYVHKILVVIEPLLIDEDYYARVEGREIISNLAKAAGLATMISTMRPDIDNMDEYVRNTTARAFAVVASALGIPSLLPFLKAVCKSKKSWQARHTGIKIVQQIAILMGCAILPHLRSLVEIIEHGLVDEQQKVRTISALAIAALAEAATPYGIESFDSVLKPLWKGIRQHRGKGLAAFLKAIGYLIPLMDAEYANYYTREVMLILIREFQSPDEEMKKIVLKVVKQCCGTDGVEANYIKTEILPPFFKHFWQHRMALDRRNYRQLVDTTVELANKVGAAEIISRIVDDLKDEAEQYRKMVMETIEKIMGNLGAADIDHKLEEQLIDGILYAFQEQTTEDSVMLNGFGTVVNALGKRVKPYLPQICGTVLWRLNNKSAKVRQQAADLISRTAVVMKTCQEEKLMGHLGVVLYEYLGEEYPEVLGSILGALKAIVNVIGMHKMTPPIKDLLPRLTPILKNRHEKVQENCIDLVGRIADRGAEYVSAREWMRICFELLELLKAHKKAIRRATVNTFGYIAKAIGPHDVLATLLNNLKVQERQNRVCTTVAIAIVAETCSPFTVLPALMNEYRVPELNVQNGVLKSLSFLFEYIGEMGKDYIYAVTPLLEDALMDRDLVHRQTASAVVQHMSLGVYGFGCEDSLNHLLNYVWPNVFETSPHVIQAVMGALEGLRVAIGPCRMLQYCLQGLFHPARKVRDVYWKIYNSIYIGSQDALIAHYPRIYNDDKNTYIRYELDYIL
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - O75533 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K6 | Ubiquitination | Uniprot | |
| K21 | Ubiquitination | Uniprot | |
| K22 | Ubiquitination | Uniprot | |
| T36 | Phosphorylation | Uniprot | |
| Y38 | Phosphorylation | Uniprot | |
| Y39 | Phosphorylation | Uniprot | |
| Y44 | Phosphorylation | Uniprot | |
| T61 | Phosphorylation | Uniprot | |
| Y70 | Phosphorylation | Uniprot | |
| S72 | Phosphorylation | Uniprot | |
| S73 | Phosphorylation | Uniprot | |
| S75 | Phosphorylation | Uniprot | |
| K81 | Ubiquitination | Uniprot | |
| Y84 | Phosphorylation | Uniprot | |
| Y101 | Phosphorylation | Uniprot | |
| Y119 | Phosphorylation | Uniprot | |
| T125 | Phosphorylation | Uniprot | |
| S129 | Phosphorylation | Uniprot | |
| K141 | Acetylation | Uniprot | |
| K141 | Ubiquitination | Uniprot | |
| T142 | Phosphorylation | P24941 (CDK2) | Uniprot |
| K146 | Ubiquitination | Uniprot | |
| Y152 | Phosphorylation | Uniprot | |
| K175 | Ubiquitination | Uniprot | |
| K182 | Ubiquitination | Uniprot | |
| S190 | Phosphorylation | Uniprot | |
| S194 | Phosphorylation | Uniprot | |
| K195 | Acetylation | Uniprot | |
| K195 | Methylation | Uniprot | |
| K195 | Ubiquitination | Uniprot | |
| T203 | Phosphorylation | Uniprot | |
| T207 | Phosphorylation | Uniprot | |
| T211 | Phosphorylation | P24941 (CDK2) | Uniprot |
| K213 | Ubiquitination | Uniprot | |
| K214 | Sumoylation | Uniprot | |
| K214 | Ubiquitination | Uniprot | |
| S216 | Phosphorylation | Uniprot | |
| S217 | Phosphorylation | Uniprot | |
| T223 | Phosphorylation | Uniprot | |
| T227 | Phosphorylation | Uniprot | |
| S229 | Phosphorylation | Uniprot | |
| R231 | Methylation | Uniprot | |
| T235 | Phosphorylation | Uniprot | |
| K240 | Ubiquitination | Uniprot | |
| S242 | Phosphorylation | Uniprot | |
| T244 | Phosphorylation | P24941 (CDK2) | Uniprot |
| T248 | Phosphorylation | P24941 (CDK2) | Uniprot |
| S251 | Phosphorylation | Uniprot | |
| T257 | Phosphorylation | Uniprot | |
| S259 | Phosphorylation | P24941 (CDK2) | Uniprot |
| T261 | Phosphorylation | P24941 (CDK2) | Uniprot |
| T267 | Phosphorylation | P24941 (CDK2) | Uniprot |
| T273 | Phosphorylation | Q13627 (DYRK1A) | Uniprot |
| T278 | Phosphorylation | Uniprot | |
| T285 | Phosphorylation | Uniprot | |
| S286 | Phosphorylation | Uniprot | |
| S287 | Phosphorylation | Uniprot | |
| T296 | Phosphorylation | Uniprot | |
| T299 | Phosphorylation | Uniprot | |
| T303 | Phosphorylation | Uniprot | |
| S308 | Phosphorylation | Uniprot | |
| T313 | Phosphorylation | P24941 (CDK2) | Uniprot |
| T316 | Phosphorylation | Uniprot | |
| R318 | Methylation | Uniprot | |
| S322 | Phosphorylation | Uniprot | |
| T326 | Phosphorylation | Uniprot | |
| T328 | Phosphorylation | Uniprot | |
| S332 | Phosphorylation | Uniprot | |
| K333 | Acetylation | Uniprot | |
| K333 | Ubiquitination | Uniprot | |
| T341 | Phosphorylation | Uniprot | |
| S344 | Phosphorylation | Uniprot | |
| S349 | Phosphorylation | Uniprot | |
| T350 | Phosphorylation | Uniprot | |
| T354 | Phosphorylation | P24941 (CDK2) | Uniprot |
| T362 | Phosphorylation | Uniprot | |
| T369 | Phosphorylation | Uniprot | |
| T371 | Phosphorylation | Uniprot | |
| S377 | Phosphorylation | Uniprot | |
| T379 | Phosphorylation | Uniprot | |
| S400 | Phosphorylation | Uniprot | |
| K413 | Sumoylation | Uniprot | |
| K413 | Ubiquitination | Uniprot | |
| Y421 | Phosphorylation | Uniprot | |
| T426 | Phosphorylation | P24941 (CDK2) | Uniprot |
| T432 | Phosphorylation | P24941 (CDK2) | Uniprot |
| T434 | Phosphorylation | Q9Y463 (DYRK1B) , P24941 (CDK2) , Q13627 (DYRK1A) | Uniprot |
| T436 | Phosphorylation | P24941 (CDK2) | Uniprot |
| T442 | Phosphorylation | Uniprot | |
| K454 | Ubiquitination | Uniprot | |
| K468 | Sumoylation | Uniprot | |
| K468 | Ubiquitination | Uniprot | |
| Y474 | Phosphorylation | Uniprot | |
| K477 | Ubiquitination | Uniprot | |
| S488 | Phosphorylation | Uniprot | |
| K493 | Ubiquitination | Uniprot | |
| K499 | Sumoylation | Uniprot | |
| K499 | Ubiquitination | Uniprot | |
| T508 | Phosphorylation | Uniprot | |
| R517 | Methylation | Uniprot | |
| S541 | Phosphorylation | Uniprot | |
| K554 | Acetylation | Uniprot | |
| K554 | Ubiquitination | Uniprot | |
| R558 | Methylation | Uniprot | |
| K562 | Acetylation | Uniprot | |
| K700 | Ubiquitination | Uniprot | |
| K733 | Ubiquitination | Uniprot | |
| Y761 | Phosphorylation | Uniprot | |
| Y764 | Phosphorylation | Uniprot | |
| K785 | Ubiquitination | Uniprot | |
| K790 | Ubiquitination | Uniprot | |
| K793 | Ubiquitination | Uniprot | |
| T808 | Phosphorylation | Uniprot | |
| K816 | Ubiquitination | Uniprot | |
| S851 | Phosphorylation | Uniprot | |
| K858 | Ubiquitination | Uniprot | |
| K866 | Ubiquitination | Uniprot | |
| K874 | Ubiquitination | Uniprot | |
| K926 | Ubiquitination | Uniprot | |
| K963 | Ubiquitination | Uniprot | |
| K1008 | Ubiquitination | Uniprot | |
| K1014 | Ubiquitination | Uniprot | |
| T1021 | Phosphorylation | Uniprot | |
| K1025 | Ubiquitination | Uniprot | |
| K1030 | Ubiquitination | Uniprot | |
| R1045 | Methylation | Uniprot | |
| C1059 | S-Nitrosylation | Uniprot | |
| K1067 | Acetylation | Uniprot | |
| K1102 | Ubiquitination | Uniprot | |
| Y1136 | Phosphorylation | Uniprot | |
| T1170 | Phosphorylation | Uniprot | |
| R1180 | Methylation | Uniprot | |
| Y1288 | Phosphorylation | Uniprot | |
| K1292 | Ubiquitination | Uniprot | |
| Y1295 | Phosphorylation | Uniprot | |
| Y1302 | Phosphorylation | Uniprot |
研究背景
Involved in pre-mRNA splicing as a component of the splicing factor SF3B complex. SF3B complex is required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. Together with other U2 snRNP complex components may also play a role in the selective processing of microRNAs (miRNAs) from the long primary miRNA transcript, pri-miR-17-92 (By similarity). May also be involved in the assembly of the 'E' complex. Belongs also to the minor U12-dependent spliceosome, which is involved in the splicing of rare class of nuclear pre-mRNA intron.
Phosphorylated. Phosphorylation occurs concomitantly with the splicing catalytic steps. Phosphorylation on Thr-244, Thr-248 and Thr-313 by cyclin-dependent kinases promotes interaction with PPP1R8 during mitosis.
Citrullinated by PADI4.
Nucleus. Nucleus speckle.
Note: During mitosis, transiently dispersed from the nuclear speckles to the cytoplasm.
Component of the B-WICH complex, at least composed of SMARCA5/SNF2H, BAZ1B/WSTF, SF3B1, DEK, MYO1C, ERCC6, MYBBP1A and DDX21. Identified in the spliceosome C complex. Component of the U11/U12 snRNPs that are part of the U12-type spliceosome. Component of splicing factor SF3B complex which is composed of at least eight subunits; SF3B1, SF3B2, SF3B3, SF3B4, SF3B5, SF3B6, PHF5A and DDX42. SF3B associates with the splicing factor SF3A and a 12S RNA unit to form the U2 small nuclear ribonucleoproteins complex (U2 snRNP). Interacts directly with the splicing factor U2AF. Within the SF3B complex interacts directly (via HEAT domain) with SF3B3, SF3B5 and (via HEAT domain) with PHF5A. Interacts directly with SF3B6 Ref.36,. The SF3B complex composed of SF3B1, SF3B2, SF3B3, SF3B4, SF3B5, SF3B6 and PHF5A interacts with U2AF2. Phosphorylated form interacts with PPP1R8. Interacts with PQBP1. Interacts with RBM17. Interacts with RBM39. Interacts with SETX. Interacts with RBM15.
Belongs to the SF3B1 family.
研究领域
· Genetic Information Processing > Transcription > Spliceosome.
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