产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF2406, RRID:AB_2845420.
展开/折叠
70Z-SHP; EC 3.1.3.48; HCP; HCPH; Hematopoietic cell phosphatase; Hematopoietic cell protein tyrosine phosphatase; Hematopoietic cell protein-tyrosine phosphatase; HPTP1C; Protein tyrosine phosphatase 1C; Protein tyrosine phosphatase non receptor type 6; Protein tyrosine phosphatase SHP1; Protein-tyrosine phosphatase 1C; protein-tyrosine phosphatase SHP 1; Protein-tyrosine phosphatase SHP-1; PTN6_HUMAN; PTP 1C; PTP-1C; PTP1C; Ptpn6; SH PTP 1; SH PTP1; SH-PTP1; SHP 1; SHP 1L; SHP1; SHP1L; tyrosine protein phosphatase non receptor type 6; Tyrosine-protein phosphatase non-receptor type 6;
抗原和靶标
Isoform 1 is expressed in hematopoietic cells. Isoform 2 is expressed in non-hematopoietic cells.
- P29350 PTN6_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MVRWFHRDLSGLDAETLLKGRGVHGSFLARPSRKNQGDFSLSVRVGDQVTHIRIQNSGDFYDLYGGEKFATLTELVEYYTQQQGVLQDRDGTIIHLKYPLNCSDPTSERWYHGHMSGGQAETLLQAKGEPWTFLVRESLSQPGDFVLSVLSDQPKAGPGSPLRVTHIKVMCEGGRYTVGGLETFDSLTDLVEHFKKTGIEEASGAFVYLRQPYYATRVNAADIENRVLELNKKQESEDTAKAGFWEEFESLQKQEVKNLHQRLEGQRPENKGKNRYKNILPFDHSRVILQGRDSNIPGSDYINANYIKNQLLGPDENAKTYIASQGCLEATVNDFWQMAWQENSRVIVMTTREVEKGRNKCVPYWPEVGMQRAYGPYSVTNCGEHDTTEYKLRTLQVSPLDNGDLIREIWHYQYLSWPDHGVPSEPGGVLSFLDQINQRQESLPHAGPIIVHCSAGIGRTGTIIVIDMLMENISTKGLDCDIDIQKTIQMVRAQRSGMVQTEAQYKFIYVAIAQFIETTKKKLEVLQSQKGQESEYGNITYPPAMKNAHAKASRTSSKHKEDVYENLHTKNKREEKVKKQRSADKEKSKGSLKRK
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P29350 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S10 | Phosphorylation | Uniprot | |
| K19 | Ubiquitination | Uniprot | |
| S26 | Phosphorylation | Uniprot | |
| K34 | Ubiquitination | Uniprot | |
| S57 | Phosphorylation | Uniprot | |
| Y61 | Phosphorylation | Uniprot | |
| Y64 | Phosphorylation | Uniprot | |
| K97 | Ubiquitination | Uniprot | |
| Y98 | Phosphorylation | Uniprot | |
| Y111 | Phosphorylation | Uniprot | |
| S140 | Phosphorylation | Uniprot | |
| K155 | Ubiquitination | Uniprot | |
| K168 | Ubiquitination | Uniprot | |
| Y176 | Phosphorylation | Uniprot | |
| T177 | Phosphorylation | Uniprot | |
| S186 | Phosphorylation | Uniprot | |
| T188 | Phosphorylation | Uniprot | |
| K195 | Ubiquitination | Uniprot | |
| K196 | Ubiquitination | Uniprot | |
| Y208 | Phosphorylation | Uniprot | |
| Y213 | Phosphorylation | Uniprot | |
| Y214 | Phosphorylation | Uniprot | |
| K232 | Ubiquitination | Uniprot | |
| K241 | Ubiquitination | Uniprot | |
| S250 | Phosphorylation | Uniprot | |
| K253 | Ubiquitination | Uniprot | |
| K257 | Ubiquitination | Uniprot | |
| K271 | Ubiquitination | Uniprot | |
| Y276 | Phosphorylation | Uniprot | |
| K277 | Acetylation | Uniprot | |
| K277 | Ubiquitination | Uniprot | |
| S285 | Phosphorylation | Uniprot | |
| R286 | Methylation | Uniprot | |
| S299 | Phosphorylation | Uniprot | |
| Y301 | Phosphorylation | Uniprot | |
| Y306 | Phosphorylation | Uniprot | |
| K308 | Ubiquitination | Uniprot | |
| K360 | Ubiquitination | Uniprot | |
| Y364 | Phosphorylation | Uniprot | |
| Y374 | Phosphorylation | Uniprot | |
| Y377 | Phosphorylation | Uniprot | |
| S378 | Phosphorylation | Uniprot | |
| K391 | Ubiquitination | Uniprot | |
| T394 | Phosphorylation | Uniprot | |
| S398 | Phosphorylation | Uniprot | |
| C453 | S-Nitrosylation | Uniprot | |
| K486 | Ubiquitination | Uniprot | |
| S496 | Phosphorylation | Uniprot | |
| Y509 | Phosphorylation | Uniprot | |
| K521 | Ubiquitination | Uniprot | |
| K522 | Ubiquitination | Uniprot | |
| S528 | Phosphorylation | Uniprot | |
| K530 | Ubiquitination | Uniprot | |
| S534 | Phosphorylation | Uniprot | |
| Y536 | Phosphorylation | P06213 (INSR) , P00519 (ABL1) , P12931 (SRC) , A0A173G4P4 (Abl fusion) , P06239 (LCK) , P07948 (LYN) | Uniprot |
| T540 | Phosphorylation | Uniprot | |
| Y541 | Phosphorylation | Uniprot | |
| K546 | Ubiquitination | Uniprot | |
| S553 | Phosphorylation | Uniprot | |
| T555 | Phosphorylation | Uniprot | |
| S556 | Phosphorylation | Uniprot | |
| S557 | Phosphorylation | Uniprot | |
| K560 | Ubiquitination | Uniprot | |
| Y564 | Phosphorylation | A0A173G4P4 (Abl fusion) , P00519 (ABL1) , P07948 (LYN) , P06239 (LCK) , P12931 (SRC) | Uniprot |
| K570 | Ubiquitination | Uniprot | |
| S582 | Phosphorylation | Uniprot | |
| S588 | Phosphorylation | Uniprot | |
| S591 | Phosphorylation | P17252 (PRKCA) , P24941 (CDK2) | Uniprot |
研究背景
Modulates signaling by tyrosine phosphorylated cell surface receptors such as KIT and the EGF receptor/EGFR. The SH2 regions may interact with other cellular components to modulate its own phosphatase activity against interacting substrates. Together with MTUS1, induces UBE2V2 expression upon angiotensin II stimulation. Plays a key role in hematopoiesis.
Phosphorylated on tyrosine residues. Binding of KITLG/SCF to KIT increases tyrosine phosphorylation (By similarity). Phosphorylation at Tyr-564 enhances phosphatase activity.
Cytoplasm. Nucleus.
Note: In neurons, translocates into the nucleus after treatment with angiotensin II (By similarity). Shuttles between the cytoplasm and nucleus via its association with PDPK1.
Isoform 1 is expressed in hematopoietic cells. Isoform 2 is expressed in non-hematopoietic cells.
Monomer. Interacts with MTUS1 (By similarity). Interacts with MILR1 (tyrosine-phosphorylated) (By similarity). Interacts with KIT (By similarity). Interacts with SIRPA/PTPNS1. Interacts with LILRB1 and LILRB2. Interacts with FCRL2 and FCRL4. Interacts with FCRL3 and FCRL6 (tyrosine phosphorylated form). Interacts with CD84. Interacts with CD300LF. Interacts with CDK2. Interacts with KIR2DL1; the interaction is enhanced by ARRB2. Interacts (via SH2 1 domain) with ROS1; the interaction is direct and promotes ROS1 dephosphorylation. Interacts with EGFR; inhibits EGFR-dependent activation of MAPK/ERK. Interacts with LYN. Interacts with the tyrosine phosphorylated form of PDPK1. Interacts with CEACAM1 (via cytoplasmic domain); this interaction depends on the monomer/dimer equilibrium and is phosphorylation-dependent (By similarity). Interacts with MPIG6B (via ITIM motif). Interacts with moesin/MSN.
The N-terminal SH2 domain functions as an auto-inhibitory domain, blocking the catalytic domain in the ligand-free close conformation.
Belongs to the protein-tyrosine phosphatase family. Non-receptor class 2 subfamily.
研究领域
· Cellular Processes > Cellular community - eukaryotes > Adherens junction. (View pathway)
· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway. (View pathway)
· Human Diseases > Infectious diseases: Parasitic > Leishmaniasis.
· Human Diseases > Cancers: Overview > Proteoglycans in cancer.
· Organismal Systems > Immune system > Natural killer cell mediated cytotoxicity. (View pathway)
· Organismal Systems > Immune system > T cell receptor signaling pathway. (View pathway)
· Organismal Systems > Immune system > B cell receptor signaling pathway. (View pathway)
限制条款
产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。
产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。
Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。产品仅供科学研究使用。不用于诊断和治疗。
产品未经授权不得转售。
Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.