| 产品: | 磷酸化 Sin1 (Thr86) 抗体 |
| 货号: | AF2407 |
| 描述: | Rabbit polyclonal antibody to Phospho-Sin1 (Thr86) |
| 应用: | WB IHC |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 78, 74kDa; 59kD(Calculated). |
| 蛋白号: | Q9BPZ7 |
| RRID: | AB_2845421 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF2407, RRID:AB_2845421.
展开/折叠
MAPKAP 1; MAPKAP1; MEKK2 interacting protein 1; MGC2745; MIP 1; MIP1; Mitogen activated protein kinase associated protein 1; Mitogen-activated protein kinase 2-associated protein 1; mSIN1; OTTHUMP00000064207; Ras inhibitor MGC2745; SAPK interacting protein 1; SAPK-interacting protein 1; SIN 1; SIN1_HUMAN; SIN1b; SIN1g; Stress activated map kinase interacting protein 1; Stress activated protein kinase interacting 1; Stress-activated map kinase-interacting protein 1; Target of rapamycin complex 2 subunit MAPKAP1; TORC2 subunit MAPKAP1;
抗原和靶标
- Q9BPZ7 SIN1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAFLDNPTIILAHIRQSHVTSDDTGMCEMVLIDHDVDLEKIHPPSMPGDSGSEIQGSNGETQGYVYAQSVDITSSWDFGIRRRSNTAQRLERLRKERQNQIKCKNIQWKERNSKQSAQELKSLFEKKSLKEKPPISGKQSILSVRLEQCPLQLNNPFNEYSKFDGKGHVGTTATKKIDVYLPLHSSQDRLLPMTVVTMASARVQDLIGLICWQYTSEGREPKLNDNVSAYCLHIAEDDGEVDTDFPPLDSNEPIHKFGFSTLALVEKYSSPGLTSKESLFVRINAAHGFSLIQVDNTKVTMKEILLKAVKRRKGSQKVSGPQYRLEKQSEPNVAVDLDSTLESQSAWEFCLVRENSSRADGVFEEDSQIDIATVQDMLSSHHYKSFKVSMIHRLRFTTDVQLGISGDKVEIDPVTNQKASTKFWIKQKPISIDSDLLCACDLAEEKSPSHAIFKLTYLSNHDYKHLYFESDAATVNEIVLKVNYILESRASTARADYFAQKQRKLNRRTSFSFQKEKKSGQQ
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9BPZ7 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S84 | Phosphorylation | Uniprot | |
| T86 | Phosphorylation | P31749 (AKT1) | Uniprot |
| K121 | Ubiquitination | Uniprot | |
| S128 | Phosphorylation | Uniprot | |
| K162 | Ubiquitination | Uniprot | |
| K166 | Ubiquitination | Uniprot | |
| S185 | Phosphorylation | Uniprot | |
| S186 | Phosphorylation | Uniprot | |
| S260 | Phosphorylation | P42345 (MTOR) | Uniprot |
| S270 | Phosphorylation | Uniprot | |
| K276 | Ubiquitination | Uniprot | |
| K302 | Ubiquitination | Uniprot | |
| S315 | Phosphorylation | Uniprot | |
| S367 | Phosphorylation | Uniprot | |
| T398 | Phosphorylation | Uniprot | |
| K428 | Ubiquitination | Uniprot | |
| S447 | Phosphorylation | Uniprot | |
| S449 | Phosphorylation | Uniprot | |
| S459 | Phosphorylation | Uniprot | |
| Y497 | Phosphorylation | Uniprot | |
| K501 | Ubiquitination | Uniprot | |
| T509 | Phosphorylation | Uniprot | |
| S510 | Phosphorylation | Uniprot | |
| S512 | Phosphorylation | Uniprot |
研究背景
Subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. Within mTORC2, MAPKAP1 is required for complex formation and mTORC2 kinase activity. MAPKAP1 inhibits MAP3K2 by preventing its dimerization and autophosphorylation. Inhibits HRAS and KRAS signaling. Enhances osmotic stress-induced phosphorylation of ATF2 and ATF2-mediated transcription. Involved in ciliogenesis, regulates cilia length through its interaction with CCDC28B independently of mTORC2 complex.
Cell membrane>Peripheral membrane protein. Cytoplasmic vesicle. Nucleus.
Ubiquitously expressed, with highest levels in heart and skeletal muscle.
All isoforms except isoform 4 can be incorporated into the mammalian target of rapamycin complex 2 (mTORC2) which contains MTOR, MLST8, PRR5, RICTOR, MAPKAP1 and DEPTOR. Contrary to mTORC1, mTORC2 does not bind to and is not sensitive to FKBP12-rapamycin. Interacts with ATF2, MAP3K2 and MAPK8. Interacts with GTP-bound HRAS and KRAS. Interacts with IFNAR2 and SGK1. Isoform 2 interacts with NBN. Isoform 1 interacts with CCDC28B.
Belongs to the SIN1 family.
研究领域
· Environmental Information Processing > Signal transduction > mTOR signaling pathway. (View pathway)
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