Phospho-SRC3 (Thr24) Antibody - #AF2412
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF2412, RRID:AB_2845426.
展开/折叠
ACTR; AIB 1; AIB-1; AIB1; Amplified in breast cancer 1; Amplified in breast cancer 1 protein; bHLHe42; CAGH 16; CAGH16; CBP interacting protein; CBP-interacting protein; Class E basic helix-loop-helix protein 42; CTG 26; CTG26; KAT13B / AIB1; MGC141848; NCOA 3; NCoA-3; Ncoa3; NCOA3_HUMAN; Nuclear receptor coactivator 3; pCIP; RAC 3; RAC-3; RAC3; Receptor associated coactivator 3; Receptor-associated coactivator 3; SRC 3; SRC-3; SRC3; Steroid receptor coactivator protein 3; Thyroid hormone receptor activator molecule 1; TNRC14; TNRC16; TRAM 1; TRAM-1; TRAM1;
抗原和靶标
Widely expressed. High expression in heart, skeletal muscle, pancreas and placenta. Low expression in brain, and very low in lung, liver and kidney.
- Q9Y6Q9 NCOA3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSGLGENLDPLASDSRKRKLPCDTPGQGLTCSGEKRRREQESKYIEELAELISANLSDIDNFNVKPDKCAILKETVRQIRQIKEQGKTISNDDDVQKADVSSTGQGVIDKDSLGPLLLQALDGFLFVVNRDGNIVFVSENVTQYLQYKQEDLVNTSVYNILHEEDRKDFLKNLPKSTVNGVSWTNETQRQKSHTFNCRMLMKTPHDILEDINASPEMRQRYETMQCFALSQPRAMMEEGEDLQSCMICVARRITTGERTFPSNPESFITRHDLSGKVVNIDTNSLRSSMRPGFEDIIRRCIQRFFSLNDGQSWSQKRHYQEAYLNGHAETPVYRFSLADGTIVTAQTKSKLFRNPVTNDRHGFVSTHFLQREQNGYRPNPNPVGQGIRPPMAGCNSSVGGMSMSPNQGLQMPSSRAYGLADPSTTGQMSGARYGGSSNIASLTPGPGMQSPSSYQNNNYGLNMSSPPHGSPGLAPNQQNIMISPRNRGSPKIASHQFSPVAGVHSPMASSGNTGNHSFSSSSLSALQAISEGVGTSLLSTLSSPGPKLDNSPNMNITQPSKVSNQDSKSPLGFYCDQNPVESSMCQSNSRDHLSDKESKESSVEGAENQRGPLESKGHKKLLQLLTCSSDDRGHSSLTNSPLDSSCKESSVSVTSPSGVSSSTSGGVSSTSNMHGSLLQEKHRILHKLLQNGNSPAEVAKITAEATGKDTSSITSCGDGNVVKQEQLSPKKKENNALLRYLLDRDDPSDALSKELQPQVEGVDNKMSQCTSSTIPSSSQEKDPKIKTETSEEGSGDLDNLDAILGDLTSSDFYNNSISSNGSHLGTKQQVFQGTNSLGLKSSQSVQSIRPPYNRAVSLDSPVSVGSSPPVKNISAFPMLPKQPMLGGNPRMMDSQENYGSSMGGPNRNVTVTQTPSSGDWGLPNSKAGRMEPMNSNSMGRPGGDYNTSLPRPALGGSIPTLPLRSNSIPGARPVLQQQQQMLQMRPGEIPMGMGANPYGQAAASNQLGSWPDGMLSMEQVSHGTQNRPLLRNSLDDLVGPPSNLEGQSDERALLDQLHTLLSNTDATGLEEIDRALGIPELVNQGQALEPKQDAFQGQEAAVMMDQKAGLYGQTYPAQGPPMQGGFHLQGQSPSFNSMMNQMNQQGNFPLQGMHPRANIMRPRTNTPKQLRMQLQQRLQGQQFLNQSRQALELKMENPTAGGAAVMRPMMQPQVSSQQGFLNAQMVAQRSRELLSHHFRQQRVAMMMQQQQQQQQQQQQQQQQQQQQQQQQQQQQQTQAFSPPPNVTASPSMDGLLAGPTMPQAPPQQFPYQPNYGMGQQPDPAFGRVSSPPNAMMSSRMGPSQNPMMQHPQAASIYQSSEMKGWPSGNLARNSSFSQQQFAHQGNPAVYSMVHMNGSSGHMGQMNMNPMPMSGMPMGPDQKYC
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9Y6Q9 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Acetylation | Uniprot | |
| T24 | Phosphorylation | P45983 (MAPK8) , Q16539 (MAPK14) | Uniprot |
| T30 | Phosphorylation | Uniprot | |
| S32 | Phosphorylation | Uniprot | |
| K35 | Acetylation | Uniprot | |
| K65 | Ubiquitination | Uniprot | |
| K97 | Ubiquitination | Uniprot | |
| S101 | Phosphorylation | Uniprot | |
| S102 | Phosphorylation | P49674 (CSNK1E) | Uniprot |
| K167 | Ubiquitination | Uniprot | |
| T184 | Phosphorylation | Uniprot | |
| K191 | Ubiquitination | Uniprot | |
| S214 | Phosphorylation | Uniprot | |
| S244 | Phosphorylation | Uniprot | |
| R251 | Methylation | Uniprot | |
| S274 | Phosphorylation | Uniprot | |
| K276 | Acetylation | Uniprot | |
| T282 | Phosphorylation | Uniprot | |
| S284 | Phosphorylation | Uniprot | |
| S287 | Phosphorylation | Uniprot | |
| S288 | Phosphorylation | Uniprot | |
| S306 | Phosphorylation | Uniprot | |
| S505 | Phosphorylation | P45983 (MAPK8) , Q16539 (MAPK14) | Uniprot |
| S509 | Phosphorylation | Uniprot | |
| S543 | Phosphorylation | P45983 (MAPK8) , Q16539 (MAPK14) | Uniprot |
| S551 | Phosphorylation | Uniprot | |
| T557 | Phosphorylation | Uniprot | |
| S563 | Phosphorylation | Uniprot | |
| S569 | Phosphorylation | Uniprot | |
| Y574 | Phosphorylation | Uniprot | |
| S601 | Phosphorylation | P48730 (CSNK1D) | Uniprot |
| K616 | Acetylation | Uniprot | |
| K619 | Acetylation | Uniprot | |
| K620 | Acetylation | Uniprot | |
| K620 | Ubiquitination | Uniprot | |
| S636 | Phosphorylation | Uniprot | |
| S640 | Phosphorylation | Uniprot | |
| S664 | Phosphorylation | Uniprot | |
| K687 | Acetylation | Uniprot | |
| S694 | Phosphorylation | Uniprot | |
| T714 | Phosphorylation | Uniprot | |
| S715 | Phosphorylation | Uniprot | |
| K723 | Sumoylation | Uniprot | |
| S728 | Phosphorylation | P06493 (CDK1) | Uniprot |
| K753 | Ubiquitination | Uniprot | |
| K786 | Sumoylation | Uniprot | |
| S794 | Phosphorylation | Uniprot | |
| S836 | Phosphorylation | Uniprot | |
| K840 | Methylation | Uniprot | |
| R849 | Methylation | Uniprot | |
| S857 | Phosphorylation | O15111 (CHUK) , Q16659 (MAPK6) , P17612 (PRKACA) | Uniprot |
| S860 | Phosphorylation | Q16539 (MAPK14) , P45983 (MAPK8) | Uniprot |
| S863 | Phosphorylation | Uniprot | |
| S866 | Phosphorylation | Uniprot | |
| S867 | Phosphorylation | P06493 (CDK1) , Q16539 (MAPK14) , P45983 (MAPK8) | Uniprot |
| K926 | Ubiquitination | Uniprot | |
| R951 | Methylation | Uniprot | |
| S1033 | Phosphorylation | Q05513 (PRKCZ) | Uniprot |
| S1042 | Phosphorylation | Q05513 (PRKCZ) | Uniprot |
| S1048 | Phosphorylation | Q05513 (PRKCZ) | Uniprot |
| T1059 | Phosphorylation | Q05513 (PRKCZ) | Uniprot |
| S1062 | Phosphorylation | Q05513 (PRKCZ) | Uniprot |
| T1064 | Phosphorylation | Q05513 (PRKCZ) | Uniprot |
| T1067 | Phosphorylation | Q05513 (PRKCZ) | Uniprot |
| K1091 | Methylation | Uniprot | |
| T1114 | Phosphorylation | Q05513 (PRKCZ) | Uniprot |
| R1171 | Methylation | Uniprot | |
| R1177 | Methylation | Uniprot | |
| R1188 | Methylation | Uniprot | |
| K1194 | Sumoylation | Uniprot | |
| S1330 | Phosphorylation | Uniprot | |
| Y1357 | Phosphorylation | P00519 (ABL1) | Uniprot |
研究背景
Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Plays a central role in creating a multisubunit coactivator complex, which probably acts via remodeling of chromatin. Involved in the coactivation of different nuclear receptors, such as for steroids (GR and ER), retinoids (RARs and RXRs), thyroid hormone (TRs), vitamin D3 (VDR) and prostanoids (PPARs). Displays histone acetyltransferase activity. Also involved in the coactivation of the NF-kappa-B pathway via its interaction with the NFKB1 subunit.
Acetylated by CREBBP. Acetylation occurs in the RID domain, and disrupts the interaction with nuclear receptors and regulates its function.
Methylated by CARM1.
Phosphorylated by IKK complex. Regulated its function. Phosphorylation at Ser-601 by CK1 promotes coactivator function.
Cytoplasm. Nucleus.
Note: Mainly cytoplasmic and weakly nuclear. Upon TNF activation and subsequent phosphorylation, it translocates from the cytoplasm to the nucleus.
Widely expressed. High expression in heart, skeletal muscle, pancreas and placenta. Low expression in brain, and very low in lung, liver and kidney.
Interacts with CARM1 (By similarity). Present in a complex containing NCOA2, IKKA, IKKB, IKBKG and the histone acetyltransferase protein CREBBP. Interacts with CASP8AP2, NR3C1 and PCAF. Interacts with ATAD2 and this interaction is enhanced by estradiol. Found in a complex containing NCOA3, AR and MAK. Interacts with DDX5. Interacts with PSMB9. Interacts with NPAS2. Interacts with NR4A3 (By similarity). Interacts with ESRRB; mediates the interaction between ESRRB and RNA polymerase II complexes and allows NCOA3 corecruitment to ESRRB, KLF4, NANOG, and SOX2 enhancer regions to trigger ESRRB-dependent gene activation involved in self-renewal and pluripotency (By similarity).
Contains three Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs. Motifs 1 and 2 are essential for the association with nuclear receptors, and constitute the RID domain (Receptor-interacting domain).
Belongs to the SRC/p160 nuclear receptor coactivator family.
研究领域
· Human Diseases > Drug resistance: Antineoplastic > Endocrine resistance.
· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)
· Human Diseases > Cancers: Specific types > Breast cancer. (View pathway)
· Organismal Systems > Endocrine system > Estrogen signaling pathway. (View pathway)
· Organismal Systems > Endocrine system > Thyroid hormone signaling pathway. (View pathway)
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